Quick Start™ Bradford Protein Assay

Quick Start™ Bradford Protein Assay

The Quick Start Bradford assay kit is a simple one step procedure. with no need to dilute standards for determining the concentration of protein in a solution.

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Overview

The Quick Start™ Bradford protein assay is a simple and accurate procedure for determining the concentration of protein in solution. The assay supplies ready-to-use dye reagent at 1x concentration and two protein standards at seven prediluted concentrations (0.125, 0.25, 0.5, 0.75, 1.0, 1.5, and 2.0 mg/ml). Protein concentration is determined in one step, with no need to dilute standards. Quick Start Bradford protein assay kits offer either bovine serum albumin or bovine γ-globulin standard sets.

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Specifications

Standard-concentration assay
 
Sample volume
100 µl
Linear range
0.125-1.5 mg/ml
Low-concentration assay
 
Sample volume
1 ml
Linear range
1.25-25 µg/ml
Assay wavelength
595 nm
Minimum incubation time
5 min
Adapted from method of
Bradford

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(Discontinued) Quick Start™ Bradford Protein Assay Kit 1
5000201
Colorimetric assay kit, includes 1x dye reagent (1 L), BSA standard (5 x 2 mg/ml); 200 standard assays or 4,000 microplate assays
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(Discontinued) Quick Start™ Bradford Protein Assay Kit 2
5000202
Colorimetric assay kit, includes 1x dye reagent (1 L), BSA standard set (2 sets of 7 standards, 0.125–2.0 mg/ml, 2 ml)
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(Discontinued) Quick Start™ Bradford Protein Assay Kit 3
5000203
Colorimetric assay kit, includes 1x dye reagent (1 L), bovine γ-globulin standard (5 x 2 mg/ml)
The following replacement is recommended:
  • Quick Start™ Bradford Protein Assay Kit 1 ( 5000201 )
  • Quick Start™ Bradford Protein Assay Kit 2 ( 5000202 )
  • Quick Start™ Bradford Protein Assay Kit 4 ( 5000204 )
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(Discontinued) Quick Start™ Bradford Protein Assay Kit 4
5000204
Colorimetric assay kit, includes 1x dye reagent (1 L), bovine γ-globulin standard set (2 sets of 7 standards, 0.125–2.0 mg/ml, 2 ml)

Accessories

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(Discontinued) Quick Start Bovine γ-Globulin Standard Set
5000209
Pkg of 2, set of 7 protein concentrations standards, 0.125–2.0 mg/ml, for use with Quick Start Bradford protein assay kits
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(Discontinued) Quick Start Bovine Serum Albumin Standard
5000206
5 x 2 ml vials, 2 mg/ml, prediluted protein concentration standard for use with Quick Start Bradford protein assay kits
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(Discontinued) Quick Start Bovine Serum Albumin Standard Set
5000207
Pkg of 2, set of 7 protein concentrations standards, 0.125–2.0 mg/ml, for use with Quick Start Bradford protein assay kits

Kits & Reagents

Documents

Number Description Options
1123
Protein Assay Applications Bibliography
1069
Colorimetric Protein Assays, Rev D
2969
Quick Start Bradford Protein Assay Product Information Sheet, Rev A
4110065
Instruction Manual, Quick Start Bradford Protein Assay, Rev A
6835
Quick Start™ Bradford Protein Assay Quick Guide, Ver A
6852
Reagent Compatibility Chart for Bio-Rad Protein Assays Quick Guide,Ver A