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GS-900 Calibrated Densitometer

GS-900 Calibrated Densitometer

The GS-900 Calibrated Densitometer is a self-calibrating 21 CFR Part 11 gel imaging system for acquiring gel, blot, and film images. The GS-900 Densitometer is an integral part of the Biologics Analysis Workflow, a complete, integrated solution.

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Overview

What is the Biologics Analysis Workflow?

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The GS-900 Calibrated Densitometer is designed to reproducibly image gels, blots, and film and quantitate proteins across a wide dynamic range. The GS-900 Densitometry System uses transmissive and reflective imaging to scan chromogenic samples at the optimal detection wavelength. The three-color red, green, and blue CCD imaging technology enables accurate, reproducible quantitation with a wide variety of common stains.

To meet cGMP environment needs, the GS-900 Densitometer offers a security edition software module for 21 CFR 11 complliance and an Installation and Operations Qualification (IQ/OQ) Kit.

Applications and Uses

  • Imaging of diverse samples on 1-D and 2-D gels, colorimetric dot and slot blots, film-based chemiluminescent blots, and autoradiograms
  • Protein quantitation for Coomassie Blue- and silver-stained gels over a wide dynamic range of 0–3.4 OD
  • Scanning large gels — oversized 29 × 33 cm imaging area
  • Rapid image acquisition and analysis with intuitive, user-friendly Image Lab Software

Features and Benefits of the GS-900 Calibrated Densitometer

  • Automatic self-calibration before each run
    • The GS-900 Calibrated Densitometer uses built-in NIST-traceable step tablets for internal calibration to ensure the accuracy of each scan
    • Internal calibration can be validated with an external NIST-traceable target to confirm accuracy and reproducibility using the GS-900 Calibrated Densitometer Installation and Operations Qualification Kit (1707994)
    • One-touch operation button for further ease of image acquisition
  • Image Lab Software greatly simplifies image analysis
    • Automated analysis saves time and ensures consistency between runs
    • Includes detailed tutorials; no previous imaging experience required to produce optimum gel and blot images
    • Flexible analysis — simply modify user settings to match SOPs and fine-tune band detection, background level, and other settings
    • Easy reporting with the ability to create customized reports
  • Meets 21 CFR Part 11 regulatory requirements with an optional Image Lab Software Security Module (1709691)
  • 16-bit precision and 36.3 μm resolution for the analysis of very close bands on a gel
  • Sealed imaging area to accommodate wet samples of variable thickness

Related Products

The GS-900 Calibrated Densitometry System and Image Lab Software are part of the Biologics Analysis Workflow — an integrated suite of products designed and validated to assess the purity or identity of biological products in a cGMP environment.

The Biologics Analysis Workflow provides:

  • Increased electrophoresis throughput
  • Simplified, sensitive, and reproducible gel staining
  • Accurate and reproducible imaging
  • Easy data analysis and report generation

Specifications

Calibration frequency
Automatic calibration before every scan
21 CRF Part 11
Yes (optional software package)
Acquisition software
ImageLab™ software ≥5.0
Light source
LED
Wavelength
400–750 nm
Absorbance range
0.0 to 3.4 OD Transmissive
0.0 to 2.7 OD RD reflective
Transmissive scan area
29 × 33 cm
Reflective scan area
29 × 33 cm
Bit depth
16-bit grayscale
Minimum pixel size
36.3 µm (700 dpi)
Image Lab™ software OS compatibility
Windows XP, 7, and 10
Connection type
USB 2.0
Imager dimensions (W x L x H)
62.7 × 37.6 × 18.5 cm
Imager weight
16.1 kg
Applications
Densitometry
Calibrated colorimetric gel and blot quantitation

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(Discontinued) GS900 Calibrated Densitometer
1707989
Gel densitometry system, includes scanner and cables, optional PC-compatible Image Lab™ software, 21 CFR part 11 and installation Qualification/Operations Module.
(Discontinued) GS-900™ Calibrated Densitometer with Biologics Analysis Workflow
1707991

Biologics Analysis Workflow product suite, includes gel densitometry system, scanner, cables, PC-compatible Image Lab™ Software, optional 21 CFR Part 11 and Instrument Qualification/Operations Qualification module

(Discontinued) GS-900 Calibrated Densitometer Regulatory Tools Package
1707993

Pkg of 1, set of protocols for validating the calibration of GS-900 calibrated densitometer (#170-7991), Image Lab Security Edition with 21 CFR Part 11 Module

(Discontinued) GS-900™ Installation Qualification/Operations Qualification (IQ/OQ) Kit
1707994
Pkg of 1, set of protocols and one NIST-traceable external step table for validating the calibration of GS-900™ calibrated densitometer (#170-7991)
(Discontinued) Image Lab Software, Security Edition, 5 licenses
17006171

FDA CFR 21 Part 11 module for Image Lab Software. Includes software and 5 licenses.

(Discontinued) Image Lab Software, Security Edition, 10 licenses
17006172

FDA CFR 21 Part 11 module for Image Lab Software. Includes software and 10 licenses.

Kits & Reagents

(Discontinued) 10x Tris/Glycine/SDS
1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water

Precast Gels

(Discontinued) 4–15% Criterion™ TGX™ Precast Midi Protein Gel, 26 well, 15 µl
5671085

Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

(Discontinued) 4–15% Criterion™ TGX™ Precast Midi Protein Gel, 18 well, 30 µl
5671084

Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

(Discontinued) 4–15% Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 µl
5671083

Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

(Discontinued) 4–20% Criterion™ TGX™ Precast Midi Protein Gel, 26 well, 15 µl
5671095

Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

(Discontinued) 4–20% Criterion™ TGX™ Precast Midi Protein Gel, 18 well, 30 µl
5671094

Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

(Discontinued) 4–20% Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 µl
5671093

Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

(Discontinued) 12% Criterion™ TGX™ Precast Midi Protein Gel, 26 well, 15 µl
5671045
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
(Discontinued) 12% Criterion™ TGX™ Precast Midi Protein Gel, 18 well, 30 µl
5671044
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
(Discontinued) 12% Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 µl
5671043
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

MW Standards

(Discontinued) Precision Plus Protein™ All Blue Prestained Protein Standards
1610373
500 µl, mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 50 applications
(Discontinued) Precision Plus Protein™ All Blue Prestained Protein Standards, value pack
1610393
2.5 ml (5 x 500 µl), mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 250 applications
(Discontinued) Precision Plus Protein Dual Color Standards, 500 µl
1610374
500 μl, mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 50 applications
(Discontinued) Precision Plus Protein™ Dual Color Standards, 2.5 ml
1610394
2.5 ml (5 x 500 µl), mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 250 applications
(Discontinued) Precision Plus Protein™ Dual Xtra Prestained Protein Standards
1610377
500 µl, mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 50 applications
(Discontinued) Precision Plus Protein™ Dual Xtra Prestained Protein Standards, value pack
1610397
2.5 ml (5 x 500 µl), mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 250 applications
(Discontinued) Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standards
1610375

500 µl, mixture of ten multicolor recombinant proteins (10–250 kD), 50 applications

(Discontinued) Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standards, value pack
1610395

2.5 ml (5 x 500 µl), mixture of ten multicolor recombinant proteins (10–250 kD), 250 applications

(Discontinued) Precision Plus Protein™ Unstained Protein Standards, Strep-tagged recombinant, 1 ml
1610363
1 ml, unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications
(Discontinued) Precision Plus Protein™ Unstained Protein Standards, Strep-tagged, recombinant, 5 ml
1610396
5 ml (5 x 1 ml), unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 500 applications

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