Overview
Residual host cell DNA (HCD) from Chinese hamster ovary (CHO) cells must be monitored during biopharmaceutical manufacturing to support product quality and safety. The Vericheck QX700TM ddPCRTM CHO Residual DNA Quantification Kit is a ddPCR-based solution designed for highly sensitive, precise quantification of residual CHO DNA in complex bioprocess intermediates. Using direct sample input and partitioning-based detection, the kit helps minimize the effects of inhibitors, reduces workflow complexity, and delivers accurate quantification without relying on a standard curve.
Features & Benefits
- Direct sample input workflow: enables quantification of residual CHO host cell DNA from complex bioprocess intermediates without DNA extraction, helping simplify sample preparation and reduce turnaround time
- High sensitivity and precise quantification: delivers femtogram-level detection and quantification of residual CHO DNA without a standard curve, supporting confidence in low-level measurements across a broad dynamic range. Partitioning helps minimize the impact of inhibitors commonly found in process samples, improving measurement robustness
- Optimized for residual DNA workflows: assay and supermix are designed to support minimal method development and are free of detectable CHO and E. coli DNA contamination. The kit accurately quantifies even fragmented residual DNA targets, supporting analysis of process-relevant sample types. A high-throughput workflow supports processing of large sample sets in a single day on the QX700 ddPCR platform
Applications & Uses
- Quantification of residual CHO host cell DNA in bioproduction intermediates
- Monitoring residual DNA clearance during purification and downstream processing workflows
- Supporting process development, comparability studies, and product quality testing with precise HCD measurements
Additional Equipment
This digital PCR supermix requires the use of one of the following instruments:
- QX700TM E Droplet Digital PCR System*
- QX700TM S Droplet Digital PCR System*
- QX700TM HT Droplet Digital PCR System*
*For Research Use Only. Not for use in diagnostic procedures.
Required Consumables
| Component | Catalog Number |
|---|---|
| RDG16 Cartridges (Bio-Rad Laboratories, Inc.) | 12025252 |
| Staticide SW12 Anti-Static Wipes (ACL, Inc.) | |
| Kimtech Science Precision Wipes (Kimberly Clark) | 05511 |
Required Software
- QX700 ddPCR System Analysis Software
- QX700 ddPCR System Control Software
Specifications
Ordering
The Vericheck QX700 ddPCR CHO Residual DNA Quantification Kit enables rapid, sensitive absolute quantification of CHO host cell DNA using the QX700 Droplet DigitalTM PCR (ddPCR) System.*
*For Research Use Only. Not for use in diagnostic procedures.
Downloads
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QX700 ddPCR System Analysis SoftwareQX700-ddPCR-System-Analysis-Software
Flexible ddPCR analysis software — built to fit your workflow
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QX700 ddPCR System Control SoftwareQX700-ddPCR-System-Control-Software
Software for QX700 instrument control with intuitive plate setup
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QX700 Supermix Configurator SoftwareQX700-Supermix-Configurator-Software
QX700 Supermix Configurator V1.5