ProteOn™ Protocol Development Kits

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Overview

ProteOn protocol development kits provide the reagents and sensor chips needed to perform and analyze data from a complete experiment using the ProteOn XPR36 protein interaction array system.
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Description

Description

ProteOn protocol development kits provide the reagents and sensor chips needed to perform and analyze data from a complete experiment using the ProteOn XPR36 protein interaction array system. New users will gain familiarity with instrument setup and operation, experiment design, and data analysis. Experienced users will find the kits valuable for system benchmarking and protocol development.

ProteOn One-shot Kinetics Kit
The interaction between the cytokine IL-2 and an IL-2 antibody demonstrates a detailed kinetic analysis in a single injection cycle (One-shot Kinetics). The kit also demonstrates a useful method for controlling ligand immobilization levels during protocol development and optimization.

The ProteOn One-shot Kinetics kit includes a ProteOn IL-2/IL-2 antibody pair, with sufficient materials to immobilize IL-2 antibody in up to six channels and to analyze a complete concentration series of IL-2 across each channel.

ProteOn Multiple Protein Interaction Kit
The interaction between five mutant TEM1 β-lactamase proteins and wild-type β-lactamase inhibitor protein(BLIP) demonstrates the power of the ProteOn XPR36 system to produce a detailed kinetic analysis of multiple simultaneous interactions and to map protein interfaces.

The ProteOn multiple protein interaction kit includes a ProteOn TEM1/BLIP protein set, with sufficient materials to immobilize five TEM1 mutant proteins in five channels and to perform a detailed kinetic analysis of the interaction of each of the five mutant proteins with BLIP.

ProteOn Protein-Small Molecule Kit
The interaction between carbonic anhydrase II and 4-carboxy benzene sulfonamide (4-CBS) demonstrates the capability of the ProteOn XPR36 system to detect low molecular weight analytes. The kit also provides a benchmark for optimal system performance.

The ProteOn protein-small molecule kit includes ProteOn carbonic anhydrase II/CBS, with sufficient materials to immobilize carbonic anhydrase II in up to six channels and analyze a complete concentration series of CBS.

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1761010
ProteOn protocol development kit, includes IL-2/IL-2 antibody pair, GLC sensor chip, amine coupling kit, 50 ml Na acetate buffer, pH 4.5
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1761030
ProteOn protocol development kit, includes carbonic anhydrase II/CBS pair, GLM sensor chip, amine coupling kit, 50 ml Na acetate buffer, pH 5.0

Documents

Documents

Number Description Options
6295
ProteOn™ Sensor Chips, Tips and Techniques, Rev A
5679
Applications of the ProteOn GLH Sensor Chip: Interactions Between Proteins and Small Molecules, Rev A
5540
Screening, Ranking, and Epitope Mapping of Anti-Human IL-9 Supernatants, Rev A
5449
Applications of the ProteOn NLC Sensor Chip: Antibody-Antigen, DNA-Protein, and Protein-Protein Interactions, Rev B
5358
Mechanisms of Protein-Protein Binding: Double-Mutant Cycle Analysis Using the ProteOn XPR36 System, Rev A
5368
Anaylsis of Multiple Protein-Protein Interactions Using the ProteOn XPR36 Protein Interaction Array System, Rev B
5367
Rapid Optimization of Immobilization and Binding Conditions for Kinetic Analysis of Protein-Protein Interactions Using the ProteOn™ XPR36 Protein Interaction Array System, Rev A
5360
Rapid and Detailed Analysis of Multiple Antigen-Antibody Pairs Using the ProteOn XPR36 Protein Interaction Array System, Rev A
3172
Rapid and Efficient Determination of Kinetic Rate Constants Using the ProteOn XPR36 Protein Interaction Array System, Rev B
5412
Antibody Characterization and Development: ProteOn XPR36 Protein Interaction Array System Application Guide, Rev C
5413
ProteOn XPR36 Hardware Product Information Sheet, Rev B
5404
ProteOn™ Sensor Chips Product Information Sheet, Rev F
5390
ProteOn XPR36 Protein Interaction Array System Brochure, Rev D
5409
Protein Interaction Analysis Ordering Information Sheet, Rev E
5434A
ProteOn XPR36 Protein Interaction Array System Folder. (Contains bulletins 3172, 5358, 5360, 5367, 5368, 5390, 5404, 5409, 5410, 5413, 5538, and 5465 DVD, depending on availability)
6161
Highly Efficient Lipoparticle Capture and SPR Binding Kinetics of a Membrane Protein Using the ProteOn XPR36 Protein Interaction Array System, Rev A
6414
ProteOn™ XPR36 Experimental Design and Application Guide, Rev B