Profinia Protein Purification System Buffers and Reagents

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Overview

While designed specifically to work with the Profinia system, these reagents also have general applicability within all stages of a purification workflow, from sample preparation to protein detection.
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Description

Description

Individually packaged reagents are also available for use with the Profinia system. While designed specifically to work with the Profinia system, these reagents also have general applicability within all stages of a purification workflow, from sample preparation to protein detection.

Profinia Bottled Buffers
For added convenience, preformulated buffers are available for native IMAC, GST, and desalting purifications.

Profinia Control Lysate
The Profinia control lysate is a lyophilized, dual-tagged 51 kD target protein that is meant to eliminate concerns over variability in purification buffer solutions or in the purification matrix itself. The control lysate facilitates system setup and initial purification and is included in every Profinia native IMAC and GST starter kit.

Urea and Glutathione Reagents
Powdered urea and glutathione reagents are available for use with denaturing IMAC and GST applications, respectively. The urea packs are ideal for solubilizing and denaturing proteins in E. coli extracts. The glutathione pack is essential for eluting GST fusion proteins from immobilized glutathione resins.

His and GST Antibodies
Histidine (His)-tagged and GST-tagged monoclonal antibodies are used to detect target protein expression of overexpressed 6x His and GST fusion proteins. They are supplied at a concentration of 1 mg/ml in phosphate buffered saline, pH 7.4 with 0.05% NaN3.

Ordering

Ordering

items

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(Discontinued) His Antibody
6200203
Pkg of 1 vial, 1 mg/ml, monoclonal antibody against histidine (His)-tagged proteins, for use with the Profinia protein purification system
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(Discontinued) GST Antibody
6200204
Pkg of 1 vial, 1 mg/ml, monoclonal antibody against GST-tagged proteins, for use with the Profinia protein purification system
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6200233
Lyophilized, dual-tagged 51 kD protein for system setup and initial purification, for use with the Profinia protein purification system
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(Discontinued) Glutathione Pack
6200202
1.23 g, powdered reagent, for denaturing GST applications on the Profinia protein purification system

Cartridges

Cartridges

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7324610
Pkg of 5, 1 ml, prepacked Ni-charged column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324612
Pkg of 1, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324614
Pkg of 5, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324620
Pkg of 5, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324622
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324624
Pkg of 5, 5 ml, prepacked affinity columns, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324646
Pkg of 2, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems
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7324647
Pkg of 4, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems
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7324648
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems
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7324600
Pkg of 5, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324602
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7325304
Pkg of 5, 10 ml, prepacked gel filtration column, 80 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™, Profinia™ and other chromatography systems
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7325312
Pkg of 1, 50 ml, prepacked gel filtration column, 80 mm x 28.2 mm, max. pressure 40 psi, for use with BioLogic™, Profinia™ and other chromatography systems
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7325314
Pkg of 5, 50 ml, prepacked gel filtration column, 80 mm x 28.2 mm, max. pressure 40 psi, for use with BioLogic™, Profinia™ and other chromatography systems

Documents

Documents

Number Description Options
6001
Rapid Validation of Purified Proteins Using Criterion Stain-Free Gels