
Overview
Nuvia aPrime 4A is a strong anion exchange – hydrophobic interaction (AEX-HIC) mixed-mode resin designed with a distinct balance of modes optimized for biomolecule interactions. The resin's ligand density and hydrophobicity are engineered to facilitate selective and reversible binding of target molecules for high purity and yield. Nuvia aPrime offers a wide design space and straightforward method development to purify even the most complex molecules.
Benefits
- Broad range of binding and elution conditions for optimal process scale-up
- Easy parameter manipulation for complex feed stream purification
- Exceptional clearance of impurities with high target recovery
Purification of Monoclonal Antibody
The Nuvia aPrime 4A Bead is designed to provide a high DBC and excellent recovery of biomolecules. The 50 µm particle size offers good resolution and high productivity while maintaining low column pressure. In this example, the resin to clear high molecular weight species from an acidic mAb (pI ~6.9) feedstream at pH 7.8, Nuvia aPrime 4A interacts with the mAb through both electrostatic and hydrophobic interactions. The mAb monomer was eluted at ~pH 6 with an overall recovery of ~90%. The high molecular weight impurities were retained until the resin was stripped with a pH 4 buffer (Figure 1).
Fig.1. 90% recovery of mAb S with Nuvia aPrime 4A Resin.
A solution sample containing (about 10 mg of target mAb in 20 mM NaPO4, pH 7.8) was injected onto a 1 ml column and eluted by buffers at pH 6. Clear separation of the impurities from the monomeric fraction was observed. AU, absorbance units.
Straightforward Method Development
A design of experiment can be used to optimize process conditions. In the example below, method development was tested for flow-through purification of a basic protein with a pI of ~8.45. The flowthrough showed excellent clearance of impurities as determined by the monomer content (Figure 2) while providing good monomer recovery (Figure 3).
Fig 2. Excellent clearance of impurities
Binding buffer pH and NaCl affect monomer content in the flow-through fraction to yield good clearance
Fig 3. High recovery
Binding buffer pH and NaCl yield high monomer recovery in the flow-through fraction.
Product Formats
Product | Catalog Number |
---|---|
Nuvia aPrime 4A Media, 25 ml | 12007397 |
Nuvia aPrime 4A Media, 100 ml | 12007396 |
Nuvia aPrime 4A Media, 500 ml | 12007379 |
Nuvia aPrime 4A Media, 5 L | 12007380 |
Nuvia aPrime 4A Media, 10 L | 12007391 |
Screening Tools
More Information
- Nuvia aPrime 4A Product Information Sheet
- Nuvia aPrime 4A Instruction Manual
- Maximize Monomer Purity and Recovery during mAb Purification Quick Guide
- Get the Best Monomer Recovery during Protein Purification Quick Guide
Specifications
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5 L, hydrophobic anion exchange resin, 50 µm particle size

10 L, hydrophobic anion exchange resin, 50 µm particle size

Pkg of 1, 1 ml, column prepacked with Nuvia aPrime 4A, 50 µm particle size


1 row of 8 columns, 200 µl, RoboColumn unit prepacked with Nuvia aPrime 4A, 50 µm particle size

1 row of 8 columns, 600 µl, RoboColumn unit prepacked with Nuvia aPrime 4A, 50 µm particle size


Pkg of 1, 1 ml, prepacked hydrophobic anion exchange column, 7 mm x 25 mm, max. pressure 45 psi, for use with NGC Systems or any chromatography system