Nuvia aPrime 4A Media

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Overview

Nuvia aPrime 4A is a hydrophobic AEX resin engineered with distinctly balanced modes to optimize biomolecule interactions. It offers a wide design space and straightforward method development delivering both high purity and yield.

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Description

Description

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Nuvia aPrime 4A is a hydrophobic anion exchange resin designed with a distinct balance of modes optimized for biomolecule interactions. The resin's ligand density and hydrophobicity are engineered to facilitate selective and reversible binding of target molecules for high purity and yield. Nuvia aPrime offers a wide design space and straightforward method development to purify even the most complex molecules.

Benefits

  • Broad range of binding and elution conditions for optimal process scale-up
  • Easy parameter manipulation for complex feed stream purification
  • Exceptional clearance of impurities with high target recovery

Purification of Monoclonal Antibody

The Nuvia aPrime 4A Bead is designed to provide a high DBC and excellent recovery of biomolecules. The 50 µm particle size offers good resolution and high productivity while maintaining low column pressure. In this example, the resin to clear high molecular weight species from an acidic mAb (pI ~6.9) feedstream at pH 7.8, Nuvia aPrime 4A interacts with the mAb through both electrostatic and hydrophobic interactions. The mAb monomer was eluted at ~pH 6 with an overall recovery of ~90%. The high molecular weight impurities were retained until the resin was stripped with a pH 4 buffer (Figure 1).


Fig.1. 90% recovery of mAb S with Nuvia aPrime 4A Resin.
A solution sample containing (about 10 mg of target mAb in 20 mM NaPO4, pH 7.8) was injected onto a 1 ml column and eluted by buffers at pH 6. Clear separation of the impurities from the monomeric fraction was observed. AU, absorbance units.

 

Straightforward Method Development

 

A design of experiment can be used to optimize process conditions. In the example below, method development was tested for flow-through purification of a basic protein with a pI of ~8.45. The flowthrough showed excellent clearance of impurities as determined by the monomer content (Figure 2) while providing good monomer recovery (Figure 3).

 


Fig 2. Excellent clearance of impurities
Binding buffer pH and NaCl affect monomer content in the flow-through fraction to yield good clearance

 


Fig 3. High recovery
Binding buffer pH and NaCl yield high monomer recovery in the flow-through fraction.

 

Product Formats

 

Product Catalog Number
Nuvia aPrime 4A Media, 25 ml 12007397
Nuvia aPrime 4A Media, 100 ml 12007396
Nuvia aPrime 4A Media, 500 ml 12007379
Nuvia aPrime 4A Media, 5 L 12007380
Nuvia aPrime 4A Media, 10 L 12007391

Screening Tools

  • Foresight CHT XT Column, 1 ml

    Foresight Nuvia aPrime 4A Column, 1 ml

    12007392

  • Foresight CHT XT Column, 5 ml

    Foresight Nuvia aPrime 4A Column, 5 ml

    12007393

  • Foresight CHT XT, 200 μl Robocolumn

    Foresight Nuvia aPrime 4A RoboColumn Unit, 200 μl

    12007394

  • Foresight CHT XT, 600 μl Robocolumn

    Foresight Nuvia aPrime 4A RoboColumn Unit, 600 μl

    12007395

  • Foresight CHT XT, 20 μl Plates

    Foresight Nuvia aPrime 4A Plates, 20 μl

    12007411

More Information

Specifications

Specifications

Base matrix composition
Macroporous highly crosslinked polymer
Functional group
Aromatic hydrophobic anion exchanger
Median particle size
50 ± 10 µm
Ligand density
100 ± 20 µeq/ml
Dynamic binding capacity*
≥50 mg/ml at 300 cm/hr
Recommended linear flow rate
50–300 cm/hr
Pressure vs. flow performance**
Under 3 bar at flow rate of 300 cm/hr
Compression factor
1.1–1.25
Shipping solution
20% ethanol + 1 M NaCl
pH stability***
Short term: 2–14
Regeneration
1 N NaOH
Sanitization
1 N NaOH
Storage condition
20% ethanol
Chemical stability
1 N NaOH, 1 M HCl, 25% acetic acid, 8 M urea, 6 M guanidine HCl, 3 M NaCl, 1% Triton X-100, 20–70% ethanol, 30% isopropanol
Shelf life††
5 years

Ordering

Ordering

items

Use the filters below to refine results!

12007397-tn.jpg
12007397
25 ml, hydrophobic anion exchange resin, 50 µm particle size
12007396-tn.jpg
12007396
100 ml, hydrophobic anion exchange resin, 50 µm particle size
12007379-tn.jpg
12007379
500 ml, hydrophobic anion exchange resin, 50 µm particle size
12007380-tn.jpg
12007380
5 L, hydrophobic anion exchange resin, 50 µm particle size
12007391-tn.jpg
12007391
10 L, hydrophobic anion exchange resin, 50 µm particle size
foresight-nuvia-q-5ml-732-4741-thumb.jpg
12007392
Pkg of 1, 1 ml, column prepacked with Nuvia aPrime 4A, 50 µm particle size
foresight-nuvia-q-5ml-732-4741-thumb.jpg
12007393
Pkg of 1, 5 ml, column prepacked with Nuvia aPrime 4A, 50 µm particle size
foresight-robocolumn-unosphere-600ul-thumb.jpg
12007394
1 row of 8 columns, 200 µl, RoboColumn unit prepacked with Nuvia aPrime 4A, 50 µm particle size
foresight-robocolumn-unosphere-600ul-thumb.jpg
12007395
1 row of 8 columns, 600 µl, RoboColumn unit prepacked with Nuvia aPrime 4A, 50 µm particle size
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12007411
2 x 96-well plates, 20 µl, filter plate prepacked with Nuvia aPrime 4A, 50 µm particle size
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12009280

Pkg of 1, 1 ml, prepacked hydrophobic anion exchange column, 7 mm x 25 mm, max. pressure 45 psi, for use with NGC Systems or any chromatography system

Documents

Documents

Number Description Options
7310
Get the Best HCP and Aggregate Clearance during mAb Purification Flier
7302
Efficient Purification of a Recombinant Protein from Escherichia coli Fermentate with Nuvia aPrime 4A Resin
7207
Effects of Buffer Composition on Protein Purification with a Hydrophobic Anion Exchange Resin, Nuvia aPrime 4A
6982
Mixed-Mode Resins/Media Selection Guide
[ Add to Cart (Free) ]
10000098359
Instruction Manual, Nuvia aPrime 4A Hydrophobic Anion Exchange Resin
7142
Nuvia aPrime 4A Media Information Sheet
7135
Maximize Monomer Purity and Recovery during mAb Purification
7136
Get the Best Monomer Recovery during Protein Purification
[ Add to Cart (Free) ]
7193
Purification of Antibodies with Nuvia aPrime 4A Hydrophobic Anion Exchange Resin
3254
Virus Reduction and Impurity Removal Potential of Nuvia aPrime 4A Resin Application Note