Nuvia aPrime 4A is a hydrophobic anion exchange resin designed with a distinct balance of modes optimized for biomolecule interactions. The resin's ligand density and hydrophobicity are engineered to facilitate selective and reversible binding of target molecules for high purity and yield. Nuvia aPrime offers a wide design space and straightforward method development to purify even the most complex molecules.
- Broad range of binding and elution conditions for optimal process scale-up
- Easy parameter manipulation for complex feed stream purification
- Exceptional clearance of impurities with high target recovery
Purification of Monoclonal Antibody
The Nuvia aPrime 4A Bead is designed to provide a high DBC and excellent recovery of biomolecules. The 50 µm particle size offers good resolution and high productivity while maintaining low column pressure. In this example, the resin to clear high molecular weight species from an acidic mAb (pI ~6.9) feedstream at pH 7.8, Nuvia aPrime 4A interacts with the mAb through both electrostatic and hydrophobic interactions. The mAb monomer was eluted at ~pH 6 with an overall recovery of ~90%. The high molecular weight impurities were retained until the resin was stripped with a pH 4 buffer (Figure 1).
Straightforward Method Development
A design of experiment can be used to optimize process conditions. In the example below, method development was tested for flow-through purification of a basic protein with a pI of ~8.45. The flowthrough showed excellent clearance of impurities as determined by the monomer content (Figure 2) while providing good monomer recovery (Figure 3).
|Nuvia aPrime 4A Media, 25 ml||12007397|
|Nuvia aPrime 4A Media, 100 ml||12007396|
|Nuvia aPrime 4A Media, 500 ml||12007379|
|Nuvia aPrime 4A Media, 5 L||12007380|
|Nuvia aPrime 4A Media, 10 L||12007391|
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5 L, hydrophobic anion exchange resin, 50 µm particle size
10 L, hydrophobic anion exchange resin, 50 µm particle size
Pkg of 1, 1 ml, prepacked hydrophobic anion exchange column, 7 mm x 25 mm, max. pressure 45 psi, for use with NGC Systems or any chromatography system