Macro-Prep High Q Resin

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Overview

Macro-Prep High Q strong anion exchange support contains quaternary amine functional groups and is ideal for purifying acidic and neutral proteins and peptides.

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Description

Description

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Macro-Prep High Q Resin is a strong anion exchange resin containing quaternary amine functional groups that is ideal for purifying acidic and neutral proteins and peptides. It is an excellent choice for rapid purification and meets the demands of analytical, semipreparative, and process-scale applications.

This methacrylate copolymer bead provides high-resolution separations at very high flow rates. Changes in pH or ionic strength of the buffer do not cause shrinking or swelling of the support. The superior mechanical and chemical stability of Macro-Prep High Q Resin make it a preferred choice over other Q supports on the market. Macro-Prep High Q Resin can be sanitized with chaotropic agents and can be autoclaved. It provides consistent and reproducible results.

Features and Benefits

  • High capacity for biomolecules
  • High resolution of complex biological mixtures
  • Rigid polymer matrix that allows high flow rates at moderate pressures
  • Hydrophilic matrix that reduces nonspecific binding
  • Macroporous beads that enhance access to ionic sites
  • High chemical, mechanical, and thermal stability

More Information

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO 9001-registered corporation.

Specifications

Specifications

Properties of Macro-Prep High Q Support
 
Type of support
Strong anion exchanger
Functional group
–N+(CH3)3
Typical dynamic binding capacity
≥37 BSA/ml
Nominal particle size
50 µm
Nominal pore diameter
1,000 Å
Recommended maximum linear flow rate
3,000 cm/hr
pH stability
1–10
Regeneration
in 1–2 M NaCl/KCl or 70% ethanol
Chemical Compatibility of Macro-Prep High Q Support
 
1% SDS
Yes
8 M guanidine-HCI
Yes
1 N HCI
Yes
100% ethanol
Yes

Ordering

Ordering

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1580040
25 ml, strong anion exchange chromatography media, 50 μm particle size
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1560040
100 ml, strong anion exchange chromatography media, 50 μm particle size
156-0041_tn.jpg
1560041
500 ml, strong anion exchange chromatography media, 50 μm particle size
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156-0042
5 L, strong anion exchange chromatography media, for analytical, semi-preparative, and process scale applications, 50 μm particle size
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156-0043
10 L, strong anion exchange chromatography media, for analytical, semi-preparative, and process scale applications, 50 μm particle size

Kits & Reagents

Kits & Reagents

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7324650
Includes one 1 ml cartridge each of UNOsphere™ S, UNOsphere Q, Macro-Prep® High Q, Macro-Prep High S, Macro-Prep DEAE
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7324120
Pkg of 5, 1 ml, prepacked ion exchange column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324122
Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324124
Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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(Discontinued) Media Sampler Pack
1580100
Includes 25 ml each of UNOsphere™ Q and UNOsphere S, Macro-Prep® DEAE, Macro-Prep High Q, and Macro-Prep High S; 5 ml of UNOsphere SUPrA™; 10 g each of CHT™ Ceramic Hydroxyapatite Types I and II, 40 μm; and 10 g of CFT™ Ceramic Fluoroapatite Type II, 40 μm
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1250561
Pkg of 6 vials, lyophilized mixture of equine myoglobin, conalbumin, chicken ovalbumin, soybean trypsin inhibitor, for ion exchange chromatography

Documents

Documents

Number Description Options
LIT271
Instruction Manual, Macro-Prep Ion Exchange Supports, Rev D
5644
Macro-Prep® High Q Support Bulletin, Rev A
5584
Bio-Scale Mini Cartridges, Product Information Sheet, Rev B
6983
Native and Recombinant Protein Purification Resins/Media Selection Card
6810
Practical Guide: Selecting the Optimal Resins for the Process Purification of Native and Recombinant Proteins, Ver A
6813
Practical Guide: Selecting the Optimal Resins for Endotoxin Depletion in Process Purification, Ver A
1913
Developing and Scaling Up a Purification Procedure With Two New Ion Exchange Supports (Poster), Rev B
1985
Clearance of Murine Leukemia Virus From a Chimeric Monoclonal Antibody Using Ion Exchange Chromatography, Rev B.
2204
Effective Removal of Negatively Charged Interfering Molecules From Proteins, Rev B
6868
Macro-Prep® Chromatography Resins Publications List, Ver A
5662
Process Chromatography Applications Laboratory Brochure, Rev B