Helios® Gene Gun System

Helios® Gene Gun System

The Helios Gene Gun is a convenient handheld device that provides direct, rapid, and versatile gene delivery into a range of targets in vivo.

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Overview

The Helios Gene Gun is a convenient handheld device that provides rapid and direct transfection into a range of targets in vivo. The unit uses an adjustable low-pressure helium pulse to sweep DNA-, RNA-, or biomaterial-coated gold microcarriers from the inner wall of a small plastic cartridge directly into target cells.

Key Features

  • Provides easy-to-use, rapid, versatile gene delivery independent of target cell type
  • Facilitates both transient and stable expression
  • Requires only small amounts of DNA and cells; no carrier DNA needed
  • Enables codelivery of more than one plasmid
  • Allows transfer of large DNA fragments
  • Targets intracellular gene delivery to many cells
  • Works for both in vitro and in vivo transformation
  • Delivers no extraneous genes or proteins

 

Helios<sup>®</sup> Gene Gun Training Video

 

Helios® Gene Gun Training Video.

This video provides an overview of the Helios Gene Gun Biolistic Particle Transfection System, including instrument setup, microcarrier and cartridge preparation, and instrument operation.

Specifications

Helios Gene Gun System
Functional
Maximum current
10 mA peak
Input voltage
9 V alkaline battery, replaceable
Battery life
1,000 discharges in continuous use
Gas pressure
600 psi maximum helium
Safety relief pressure
700 ± 35 psi at regulator assembly
Regulator adjustment
800 psi limit maximum
Discharges
12 per cylinder, mechanical indexing
Environmental
Operating temperature
10–32°C (50–90°F); 30–80% humidity
Storage temperature
0–60°C (32–110°F); 10–90% humidity
Physical
Dimensions (W x D x H) 
20 x 25 cm (8 x 10"; approximate)
Weight 
1.43 kg (3.15 lb)

Use

For Research Use Only. Not for use in diagnostic procedures.
Tubing Prep Station
Electrical
Maximum current
62 mA/125 mA peak
Input voltage
220/240 V or 100/120 V
Functional
Input frequency
50/60 Hz
Safety relief pressure
30 ± 1.5 psi at regulator assembly
Speed
30 rpm nominal
Tubing fill
Manual
Physical
Construction
Aluminium and acrylic
Dimensions (W x D x H) 
85 x 10 cm (33.5 x 4"; approximate)
Weight 
5.08 kg (11.2 lb)

Ordering

items

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(Discontinued) Helios Gene Gun Kit
1652411
100/120 V, handheld biolistic system, includes gene gun, 5 O-rings, 5 barrel liners, 5 white cartridge holders, cartridge extractor tool, 9 V battery.
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1652431

100/120 V, handheld biolistic system, includes helium hose assembly with regulator, tubing prep station (#1652418), syringe kit, Tefzel tubing, tubing cutter, optimization kit (#1652424)

Accessories

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(Discontinued) Helium Hose Assembly
1652412
Pkg of 1, Helios gene gun accessory, connects to helium source for safety and consistent helium delivery, with Swagelok quick-connect fittings
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(Discontinued) Helium Regulator
1652413
Pkg of 1, pressure regulator for use with Helios gene gun, CGA 580 female fitting (U.S. standard) with pressure relief valve, max. pressure 2,600 psi
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1652414
Pkg of 1, pressure regulator for use with Helios gene gun, CGA 580 female fitting (U.S. standard) with pressure relief valve, max. pressure 400 psi
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(Discontinued) Syringe Kit
1652421
Kit for loading gold suspension into Tefzel tubing, includes syringe adaptor tubing, 5 x 10 ml syringes, syringe adaptor fitting, 5 Luer-Lok fittings
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1652424
Optimization kit for the Helios gene gun, includes 0.6 μm, 1.0 μm and 1.6 μm gold microcarriers, PVP, desiccant, 5 cartridge storage vials, 50 feet of Tefzel tubing, for 1,000 samples
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(Discontinued) Cartridge Kit
1652440
Kit for preparing Helios gene gun cartridges, includes 0.5 g PVP, 5 desiccant pellets, 5 cartridge collection/storage vials, 15 m of Tefzel tubing
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1652425
Pkg of 1, nitrogen regulator, for drying Tefzel tubing during Helios gene gun sample preparation
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(Discontinued) Tefzel Tubing
1652441
15 m, tubing for coating with DNA- or RNA-gold microcarrier complexes, used with the Helios® gene gun, .125" (3.175 mm) OD, .093" (2.36 mm) ID

Microcarriers

Documents

Number Description