EXQuest Spot Cutter

Image
lsr_exquest_spotcutter.jpg

Overview

The ExQuest™ Spot Cutter has been discontinued. We will continue to provide support for ExQuest owners until March, 2020.
Read full description Ordering Information

Description

Description

Discontinued Product
The ExQuest™ Spot Cutter has been discontinued. We will continue to provide support for ExQuest owners until March, 2020.

 

The EXQuest™ spot cutter is a precision instrument that accurately locates and excises protein bands or spots from 1-D and 2-D gels or blots. It then loads them into 96- and 384-well microplates or 96-tube racks for downstream processing and analysis. The EXQuest spot cutter is designed to be both robust in construction and simple to operate, reducing manual labor and improving throughput of protein spots for analysis.

The EXQuest spot cutter is a fully integrated system controlled through PDQuest™ 2-D analysis software or Quantity One® 1-D analysis software. It consists of an enclosure, an imaging system, a fluidics system, robotics, sensors, a cutting head, a gel tray, a microplate rack, and a wash station.

Versatile Excision Capability

The EXQuest spot cutter allows use of any common proteome separation and staining methods:

  • Freestanding 2-D and 1-D SDS-PAGE gels
  • Plastic- or glass-backed 2-D and 1-D gels
  • PVDF and nitrocellulose membrane blots
  • Gels or membranes stained for proteins with visible stains (such as silver and Coomassie blue stains) or fluorescent stains (such as Flamingo™ fluorescent gel stain and SYPRO Ruby protein stain)

Big Performance on the Bench

  • Accommodates any commercially available large or mini format gel or PVDF membrane blot in a small bench footprint
  • PDQuest software automatically selects spots in order from lowest to highest amount of protein, minimizing the chance of carryover contamination from spot to spot
  • Quantity One software provides convenient tools for 1–D gel cutting
  • Cutting tip produces precise cuts without damage or distortion on gels or PVDF membranes
  • Cuts up to 4 gels at a time at up to 600 spots per hour
  • System accuracy of ±0.1 mm ensures accurate excision of even small, closely grouped spots
  • Error-free data capture from image analysis to mass spectrometry
  • Built-in wash wells provide customized tip washing between cuts for extra contamination control
  • Quick-change settings to switch between UV and visible light modes
  • Compatible with ProteomeWorks™ and ProteomeWorks Plus spot cutters

 

Specifications

Specifications

System accuracy
±0.1 mm (100 µm)
Excision speed
600 spots per hour
Excision platform
Accommodates one large gel (e.g., PROTEAN® Plus handcast gels) or up to four small gels (e.g., Criterion™ gels) without the need for trimming
Pickup rate
>99.5% spot pickup on first cut
CCD camera
1,600 x 1,200 imaging pixel resolution, 100 µm resolution, 12-bit, cooled to 0°C
Excision tip nominal internal diameter
1.5 mm, 1.0 mm, and optional membrane cutting tip
Excision surfaces
Backed or free gels 1-D and 2-D gels 0.5–2.0 mm thick; visible light applications (e.g., Coomassie blue, silver stain); UV light applications (e.g., Flamingo™, SYPRO Ruby); PVDF and nitrocellulose membrane blots
Operating temperature
15–30°C
Operating humidity range
12–85%, noncondensing
Dimensions (without computer) (W x D x H)
91 x 69 x 66 cm (35.8 x 27.2 x 26.0"). Requires 38 cm clearance above the front of the instrument and 25 cm clearance on left side of instrument
Weight
Instrument only, 76 kg (168 lb)
Minimum computer requirements (computer not included)
Microsoft Windows 2000 or XP operating system (1.2 GHz), 2.1 GB hard drive, 512 MB RAM, screen resolution 1,024 x 768, 2 MB video RAM
Connection type
USB

Ordering

Ordering

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165-7200_tn.jpg
1657201
Gel excision instrument, includes PC, enclosure, imaging system, fluidics system, robotics, sensors, cutting head, gel tray, microplate track, wash station

Accessories

Accessories

165-7202_tn.jpg
(Discontinued) Cutting Tip
1657202
Pkg of 1, 1.0 mm cutting tip, for use with EXQuest spot cutter (#165-7200, #165-7201)
165-7203_tn.jpg
(Discontinued) Cutting Tip
1657203
Pkg of 1, 1.5 mm cutting tip, for use with EXQuest spot cutter (#165-7200, #165-7201)
165-7204_tn.jpg
(Discontinued) Glass Bottle
1657204
Pkg of 1, 1 L glass bottle for use with EXQuest spot cutter (#165-7200, #165-7201)
165-7205_tn.jpg
(Discontinued) Calibration Pucks
1657205
Pkg of 10, calibration puck for use with EXQuest spot cutter (#165-7200, #165-7201)
165-7206_tn.jpg
1657206
Pkg of 1, cutting head with 1.0 mm tip, for use with EXQuest spot cutter (#165-7200, #165-7201)
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(Discontinued) Membrane Cutting Tip
1657207
Pkg of 1, 1.0 mm cutting tip, for use with membrane cutting head (#165-7206) on EXQuest spot cutter (#165-7200, #165-7201)
165-7208_tn.jpg
(Discontinued) Gel Cutting Sheets
1657208
Pkg of 15, gel cutting sheet, for use with EXQuest spot cutter (#165-7200, #165-7201)
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(Discontinued) Gel Holding Clips
1657209
Pkg of 2, gel holding clip for use with EXQuest spot cutter (#165-7200, #165-7201)
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(Discontinued) Calibration Target
1657210
Pkg of 1, calibration target for use with EXQuest spot cutter (#165-7200, #165-7201)
165-7211_tn.jpg
(Discontinued) Camera Target
1657211
Pkg of 1, camera target for use with EXQuest spot cutter (#165-7200, #165-7201)
165-7212_tn.jpg
(Discontinued) Micro Tubes
1657212
Pkg of 20, 1.5 ml, micro tube for use with EXQuest spot cutter (#165-7200, #165-7201)
165-7214_tn.jpg
(Discontinued) Bottle Holder
1657214
Pkg of 1, bottle holder for use with EXQuest spot cutter (#165-7200, #165-7201)
165-7215_tn.jpg
(Discontinued) Gel Tray
1657215
Pkg of 1, gel tray for use with EXQuest spot cutter (#165-7200, #165-7201)
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1657216
Pkg of 1, transilluminator lamp for use with EXQuest spot cutter (#165-7200, #165-7201)
165-7217_tn.jpg
1657217
Pkg of 20, 96-well microplate, for use with EXQuest spot cutter (#165-7200, #165-7201)
165-7218_tn.jpg
(Discontinued) Ferrule
1657218
Pkg of 10, 10-32, 1/16" OD, ferrule for use with EXQuest spot cutter (#165-7200, #165-7201)
165-7219_tn.jpg
(Discontinued) Bar Code Reader
1657219
Pkg of 1, bar code reader for use with EXQuest spot cutter (#165-7200, #165-7201)
(Discontinued) Microplate Holder
1657220
Pkg of 1, microplate holder for use with EXQuest spot cutter (#165-7200, #165-7201)
170-9630_tn.jpg
1709630
1-user license, 2-D analysis software, provides advanced functionality

Documents

Documents

Number Description Options
3194
EXQuest Spot Cutter Brochure, Rev A
4000129-14
Instruction Manual, The Discovery Series: PDQuest 2-D Analysis Software, Rev A
10001531
Instruction Manual, EXQuest™ Spot Cutter, Rev A
3097
Imaging and Analysis: Tools for Acquisition and Analysis of Protein Expression Data Brochure, Rev B