Overview
The kit includes a reference DNA plasmid material at a known concentration, certified by the Australian National Measurement Institute (NMI), system check assay, supermix, and nuclease-free water. Using QX manager or QX ONE software, users can easily run a ddPCR plate and quickly obtain l instrument performance results with the plug-and-play excel spreadsheet validated for the solution.
Features and Benefits
- Multi-Channel Verification:
Confirms accurate performance across all detection channels simultaneously. - Plate-to-Plate Reproducibility Testing:
Enables assessment of system consistency across multiple runs and plates. - Ready-to-Use Format:
Pre-mixed, pre-validated reagents streamline workflows and reduce setup errors. - Comprehensive Workflow Integration:
Designed to fit seamlessly into routine ddPCR workflows and instrument qualification protocols.
Included Components
Configuration Options (or Instrument Compatibility)
| (Bundles or different component sets) | Catalog Number |
|---|---|
| QX600 Droplet Digital PCR Reader | 12013328 |
| QX Manager Software, Regulatory Edition | 12012172 |
| QX Manager Software, Standard Edition | 12012171 |
| QX Manager, Premium Edition | 12018108 |
| QXONE Droplet Digital PCR System | 12006536 |
| QX200 AutoDG Droplet Digital PCR System consisting of: QX200 Droplet Reader Automated Droplet Generator |
1864100 1864003 1864101 |
| QX Manager Software, Regulatory Edition | 12012172 |
| QX Manager Software, Standard Edition | 12012171 |
| QX ONE Software, Regulatory Edition | 12012078 |
| QX Manager, Premium Edition | 12018108 |
Performance Specifications
- Accuracy within ± 20% of the NMI standard material’s target concentration
- Precision in concentration measurement of ≤ 10% CV
- ≤2 Wells per plate under 10,000 droplets
- Average droplet count per plate ≥15,000
Kit Features
- Verification of all channels in a single run
- Standards verified by orthogonal methods
- Simplified data analysis with plug-and-play Excel spreadsheet
- 96 wells/ kit
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To fully harness the power of ddPCR, it's essential to confirm that instruments are performing as expected across all optical channels and over time. Routine verification minimizes variability, and ensures reproducibility from plate to plate.
To fully harness the power of ddPCR, it's essential to confirm that instruments are performing as expected across all optical channels and over time. Routine verification minimizes variability, and ensures reproducibility from plate to plate.
Downloads
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ddPCR Quantification Standard Kits_Analysis_Worksheet_v1.07ddPCR Quantification Standard Kits_Analysis_Worksh