ddSEQ Single-Cell 3' RNA-Seq Kit & Omnition Analysis Software

ddSEQ Single-Cell 3' RNA-Seq Kit & Omnition Analysis Software

The ddSEQ 3’ RNA-Seq Library Prep Kit allows researchers to profile gene expression with single-cell precision.

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Overview

Download Omnition

Omnition Analysis Software Version 1.1.0 is a robust, efficient open-source analysis pipeline for analyzing, visualizing, and reporting data from the ddSEQ Single-Cell 3' RNA-Seq assay, the ddSEQ Single-Cell ATAC-Seq Kit, and ddSEQ-deployed combinatorial indexing ATAC protocols.

The ddSEQ Single-Cell 3’ RNA-Seq Kit and Omnition Analysis Software, together, enables streamlined whole transcriptome analysis (WTA) at the single-cell level. Leveraging Bio-Rad’s ddSEQ droplet technology, this assay is able to capture and profile hundreds to thousands of single cells with high sensitivity and recovery.

Features & Benefits

  • Simple & fast encapsulation — Cells and nuclei are encapsulated in under 5 minutes using ddSEQ droplet partitioning technology
  • Essential performance — Delivers the required sensitivity and utilization with low multiplet rates, ensuring confidence in successful 3' RNA-seq applications
  • Adjustable throughput — Flexible processing of 500 to 7,000 cell output per sample, accommodating a range of scale
  • Streamlined assay workflow — Reduced workflow time with convenient stopping points, enabling completion within a day or at your convenience
  • Advanced informatics — Efficient and flexible informatics tool, featuring batch pipeline analysis of samples and a comprehensive single-cell metrics report, powered by Omnition Analysis Software

Applications & Uses

  • Uncover cellular heterogeneity in biological samples and tissues
  • Identify rare or novel cell types associated with pathogenesis or as biomarkers
  • Understand developmental pathways and trajectories

Included Components

  • ddSEQ Single-Cell 3' RNA-Seq Kit (8 reactions)
    • ddSEQ Single-Cell 3' RNA-Seq cDNA Module [includes: Droplet Generation Module (4°C) and RT Module (–20°C)]
    • ddSEQ Single-Cell 3' RNA-Seq Library Module
    • ddSEQ M Cartridges (2 cartridges)
  • Omnition Analysis Software version 1.1+

Configuration Options (or Instrument Compatibility)

(Bundles or different component sets) Catalog Number
ddSEQ Single-Cell Isolator 12004336
ddSEQ Single-Cell 3' RNA-Seq Dual Index Module 12020461
ddSEQ M Cartridges 12009359

Related Products

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Reagents and consumables for the ddSEQ Single-Cell 3' RNA-Seq Kit
17009671

Pkg of 1, complete reagents and consumables for the ddSEQ Single-Cell 3' RNA-Seq Kit (8 reactions), includes, ddSEQ Single-Cell 3' RNA-Seq cDNA Module (12020410), ddSEQ Single-Cell 3' RNA-Seq Library Module (12020413), and ddSEQ M Cartridges (12009359) for use with the ddSEQ Single-Cell Isolator

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ddSEQ Single-Cell 3 RNA-Seq cDNA Module
17010272

cDNA module for the ddSEQ Single-Cell 3' RNA-Seq Kit, for encapsulating cells and generating cDNA using the ddSEQ Single-Cell Isolator (8 reactions) 

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ddSEQ Single-Cell 3' RNA-Seq Library Module
12020413

Library module for the ddSEQ Single-Cell 3' RNA-Seq Kit, for preparing libraries from cDNA (8 reactions) 

Accessories

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12005115-96-Well-Plate-Cooling-Block_tn.jpg
12004336

ddSEQ Single-Cell Isolator is a droplet-based single cell isolation system for single-cell gene expression and gene regulation studies. The system includes the isolator, test cartridges and the cartridge holder

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12009359-th.jpg
(Discontinued) ddSEQ M Cartridges
12009359

Pkg of 2, cartridges for use with the ddSEQ Single-Cell Isolator, the ddSEQ Single-Cell 3' RNA-seq Kit and the ddSEQ Single-Cell ATAC-Seq Library Prep Kit

Kits & Reagents

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ddSEQ Single-Cell 3' RNA-Seq Dual Index Module
12020461

Two plates of 24 indexed primer pairs for use with ddSEQ Single-Cell 3' RNA-Seq Kits. Each plate contains 24 unique dual indexed primers plated in 96-well plates, one plate is for cDNA library prep and one plate is for deconvolution oligo (DO) library prep.

Documents

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