Overview
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Omnition Analysis Software Version 1.1.0 is a robust, efficient open-source analysis pipeline for analyzing, visualizing, and reporting data from the ddSEQ Single-Cell 3' RNA-Seq assay, the ddSEQ Single-Cell ATAC-Seq Kit, and ddSEQ-deployed combinatorial indexing ATAC protocols.
The ddSEQ Single-Cell 3’ RNA-Seq Kit and Omnition Analysis Software, together, enables streamlined whole transcriptome analysis (WTA) at the single-cell level. Leveraging Bio-Rad’s ddSEQ droplet technology, this assay is able to capture and profile hundreds to thousands of single cells with high sensitivity and recovery.
Features & Benefits
- Simple & fast encapsulation — Cells and nuclei are encapsulated in under 5 minutes using ddSEQ droplet partitioning technology
- Essential performance — Delivers the required sensitivity and utilization with low multiplet rates, ensuring confidence in successful 3' RNA-seq applications
- Adjustable throughput — Flexible processing of 500 to 7,000 cell output per sample, accommodating a range of scale
- Streamlined assay workflow — Reduced workflow time with convenient stopping points, enabling completion within a day or at your convenience
- Advanced informatics — Efficient and flexible informatics tool, featuring batch pipeline analysis of samples and a comprehensive single-cell metrics report, powered by Omnition Analysis Software
Applications & Uses
- Uncover cellular heterogeneity in biological samples and tissues
- Identify rare or novel cell types associated with pathogenesis or as biomarkers
- Understand developmental pathways and trajectories
Included Components
- ddSEQ Single-Cell 3' RNA-Seq Kit (8 reactions)
- ddSEQ Single-Cell 3' RNA-Seq cDNA Module [includes: Droplet Generation Module (4°C) and RT Module (–20°C)]
- ddSEQ Single-Cell 3' RNA-Seq Library Module
- ddSEQ M Cartridges (2 cartridges)
- Omnition Analysis Software version 1.1+
Configuration Options (or Instrument Compatibility)
| (Bundles or different component sets) | Catalog Number |
| ddSEQ Single-Cell Isolator | 12004336 |
| ddSEQ Single-Cell 3' RNA-Seq Dual Index Module | 12020461 |
| ddSEQ M Cartridges | 12009359 |
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Pkg of 1, complete reagents and consumables for the ddSEQ Single-Cell 3' RNA-Seq Kit (8 reactions), includes, ddSEQ Single-Cell 3' RNA-Seq cDNA Module (12020410), ddSEQ Single-Cell 3' RNA-Seq Library Module (12020413), and ddSEQ M Cartridges (12009359) for use with the ddSEQ Single-Cell Isolator
cDNA module for the ddSEQ Single-Cell 3' RNA-Seq Kit, for encapsulating cells and generating cDNA using the ddSEQ Single-Cell Isolator (8 reactions)
Library module for the ddSEQ Single-Cell 3' RNA-Seq Kit, for preparing libraries from cDNA (8 reactions)
Accessories
ddSEQ Single-Cell Isolator is a droplet-based single cell isolation system for single-cell gene expression and gene regulation studies. The system includes the isolator, test cartridges and the cartridge holder
Pkg of 2, cartridges for use with the ddSEQ Single-Cell Isolator, the ddSEQ Single-Cell 3' RNA-seq Kit and the ddSEQ Single-Cell ATAC-Seq Library Prep Kit
Kits & Reagents
Two plates of 24 indexed primer pairs for use with ddSEQ Single-Cell 3' RNA-Seq Kits. Each plate contains 24 unique dual indexed primers plated in 96-well plates, one plate is for cDNA library prep and one plate is for deconvolution oligo (DO) library prep. Indexes are identical between plates.