ddSEQ Single-Cell 3' RNA-Seq Kit & Omnition Analysis Software

ddSEQ Single-Cell 3' RNA-Seq Kit & Omnition Analysis Software

The ddSEQ 3’ RNA-Seq Library Prep Kit allows researchers to profile gene expression with single-cell precision.

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Overview

Download Omnition

Omnition Analysis Software Version 1.1.0 is a robust, efficient open-source analysis pipeline for analyzing, visualizing, and reporting data from the ddSEQ Single-Cell 3' RNA-Seq assay, the SureCell ATAC-Seq assay, and ddSEQ-deployed combinatorial indexing ATAC protocols.

The ddSEQ Single-Cell 3’ RNA-Seq Kit and Omnition Analysis Software, together, enables streamlined whole transcriptome analysis (WTA) at the single-cell level. Leveraging Bio-Rad’s ddSEQ droplet technology, this assay is able to capture and profile hundreds to thousands of single cells with high sensitivity and recovery.

Features & Benefits

  • Simple & fast encapsulation — Cells and nuclei are encapsulated in under 5 minutes using ddSEQ droplet partitioning technology
  • Essential performance — Delivers the required sensitivity and utilization with low multiplet rates, ensuring confidence in successful 3' RNA-seq applications
  • Adjustable throughput — Flexible processing of 500 to 5,000 cell output per sample, accommodating a range of scale
  • Streamlined assay workflow — Reduced workflow time with convenient stopping points, enabling completion within a day or at your convenience
  • Advanced informatics — Efficient and flexible informatics tool, featuring batch pipeline analysis of samples and a comprehensive single-cell metrics report, powered by Omnition Analysis Software

Applications & Uses

  • Uncover cellular heterogeneity in biological samples and tissues
  • Identify rare or novel cell types associated with pathogenesis or as biomarkers
  • Understand developmental pathways and trajectories

Included Components

  • ddSEQ Single-Cell 3' RNA-Seq Kit (8 reactions)
    • ddSEQ Single-Cell 3' RNA-Seq cDNA Module [includes: Droplet Generation Module (4°C) and RT Module (–20°C)]
    • ddSEQ Single-Cell 3' RNA-Seq Library Module
    • ddSEQ M Cartridges (2 cartridges)
  • Omnition Analysis Software version 1.1+

Configuration Options (or Instrument Compatibility)

(Bundles or different component sets) Catalog Number
ddSEQ Single-Cell Isolator 12004336
ddSEQ Single-Cell 3' RNA-Seq Dual Index Module 12020461
ddSEQ M Cartridges 12009359

Related Products

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Reagents and consumables for the ddSEQ Single-Cell 3' RNA-Seq Kit
17009671

Pkg of 1, complete reagents and consumables for the ddSEQ Single-Cell 3' RNA-Seq Kit (8 reactions), includes, ddSEQ Single-Cell 3' RNA-Seq cDNA Module (12020410), ddSEQ Single-Cell 3' RNA-Seq Library Module (12020413), and ddSEQ M Cartridges (12009359) for use with the ddSEQ Single-Cell Isolator

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ddSEQ Single-Cell 3 RNA-Seq cDNA Module
17010272

cDNA module for the ddSEQ Single-Cell 3' RNA-Seq Kit, for encapsulating cells and generating cDNA using the ddSEQ Single-Cell Isolator (8 reactions) 

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ddSEQ Single-Cell 3' RNA-Seq Library Module
12020413

Library module for the ddSEQ Single-Cell 3' RNA-Seq Kit, for preparing libraries from cDNA (8 reactions) 

Accessories

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12005115-96-Well-Plate-Cooling-Block_tn.jpg
12004336

ddSEQ Single-Cell Isolator is a droplet-based single cell isolation system for single-cell gene expression and gene regulation studies. The system includes the isolator, test cartridges and the cartridge holder

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12009359-th.jpg
(Discontinued) ddSEQ M Cartridges
12009359

Pkg of 2, cartridges for use with the ddSEQ Single-Cell Isolator, the ddSEQ Single-Cell 3' RNA-seq Kit and the SureCell ATAC-Seq Library Prep Kit

Kits & Reagents

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ddSEQ Single-Cell 3' RNA-Seq Dual Index Module
12020461

Two plates of 24 indexed primer pairs for use with ddSEQ Single-Cell 3' RNA-Seq Kits. Each plate contains 24 unique dual indexed primers plated in 96-well plates, one plate is for cDNA library prep and one plate is for deconvolution oligo (DO) library prep. Indexes are identical between plates.

Documents

Number Description Options
3684
ddSEQ Single-Cell 3' RNA-Seq Assay Flier
[ Add to Cart (Free) ]
10000167449
ddSEQ Single-Cell 3' RNA-Seq Kit User Guide