Bio-Plex Pro Human Acute Phase Multiplex Assays

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Overview

Disease research involving the study of inflammation, sepsis, cardiovascular disease, and diabetes often requires the measurement of multiple acute phase biomarkers. The Bio-Plex Pro human acute...

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Description

Description

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Disease research involving the study of inflammation, sepsis, cardiovascular disease, and diabetes often requires the measurement of multiple acute phase biomarkers. Bio-Plex Pro human acute phase immunoassays deliver accurate and reproducible measurements of nine of the most commonly measured biomarkers, using the Bio-Plex 200 system. The multiplex immunoassays have optimal performance in serum, plasma, and tissue culture supernatant. Bio-Plex offers a 4-plex panel and a 5-plex panel, as well as a complete kit which contains both panels.

The 5-plex panel detects:

  • Ferritin
  • Fibrinogen
  • Procalcitonin
  • Serum amyloid A
  • Tissue plasminogen activator

The 4-plex panel detects:

  • α-2-macroglobin
  • C-reactive protein (CRP)
  • Haptoglobin
  • Serum amyloid P

Dividing the targets into two kits of distinct sample dilution requirements reduces the need to expend assay reagents on multiple dilutions. These assays are based on 8.0 µm magnetic beads making them compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

Key Features

  • Allows detection of 9 human acute phase biomarkers in 2 assays
  • Provides excellent sensitivity with both the automated washing system and a standard vacuum manifold
  • Eliminates manual filtration steps (with automated washing), increasing throughput and reproducibility
  • Includes controls for generating QC samples

Additional Neccessary Reagents

If a complete kit is purchased, no additional reagents are required. If the panels are purchased alone, the following additional items are needed to perform a Bio-Plex Pro human acute phase assay:

  • Bio-Plex Pro human acute phase reagent kit — includes assay reagents and instructions required to prepare samples and acquire data
  • Bio-Plex Pro human acute phase diluent kit — includes diluents for diluting samples, standards, and controls (one diluent kit is sufficient to perform both the 5-plex and the 4-plex assays)

Instrument Compatibility

This product is not compatible with the Bio-Plex MAPGIX and Bio-Plex 3D systems due to the bead size.

More Information

See how Bio-Plex instruments and assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

Specifications

Specifications

Species
Human
Immunoassay for
Acute phase
Format
Premixed panel
Custom assay options
Not available

Ordering

Ordering

items

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171A4S07M
1 x 96-well, includes coupled beads, detection antibodies, standards, controls, reagents and diluents for detecting 9 human acute phase biomarkers
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171A4C09M
1 x 96-well, includes coupled beads, detection antibodies, standards, controls, reagents and diluents for detecting 4 human acute phase biomarkers
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171A4C07M
1 x 96-well, includes coupled beads, detection antibodies, standards, controls, reagents and diluents for detecting 5 human acute phase biomarkers
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171A4007M
1 x 96-well, includes coupled beads, detection antibodies, standards, controls for detecting 5 human acute phase biomarkers, requires acute phase reagent kit, diluent kit
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171A4008M
10 x 96-well, includes coupled beads, detection antibodies, standards, and controls for detecting 5 human acute phase biomarkers, requires acute phase reagent kit and 10 diluent kits
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171A4009M
1 x 96-well, includes coupled beads, detection antibodies, standards, controls for detecting 4 human acute phase biomarkers, requires acute phase reagent kit and diluent kit
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171A4010M
10 x 96-well, includes coupled beads, detection antibodies, standards, and controls for detecting 4 human acute phase biomarkers, requires acute phase reagent kit and 10 diluent kits

Accessories

Accessories

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171304050
1 x 96-well, includes 96-well filter plate, assay and wash buffers, detection antibody diluent, streptavidin-PE, sealing tape
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171304051
10 x 96-well, includes 10 x 96-well filter plate, assay and wash buffers, detection antibody diluent, streptavidin-PE, sealing tape
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171305050
1 x 96-well, includes 96-well filter plate, 10 ml serum-based diluent, 300 ml serum-free diluent; for one 5-plex and one 4-plex assay

Documents

Documents

Number Description Options
5405
Bio-Plex® Multiplex System Brochure, Ver E
[ Add to Cart (Free) ]
10011597
Bio-Plex™ Pro Acute Phase Instruction Manual Rev A
5650
Bio-Plex Pro™ Human Acute Phase Assay Panel Product Sheet, Rev C
6335
Bio-Plex Multiplex Immunoassays Analyte Guide
6457
The Bio-Plex Success Guide for Multiplex Immunoassays, Rev B
6458
Bio-Plex Success Guide/Assay Sample Prep, Rev B
3235
Comparison of Bio-Plex Pro Assay Performance Using the Bio-Plex 200 and Luminex xMAP INTELLIFLEX Systems