QXDx AutoDG ddPCR System

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Overview

Designed for high-throughput processing of samples in a 96-well format, this system simplifies the ddPCR workflow and eliminates user-to-user variability in IVD applications.

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Description

Description

 

The QXDx AutoDG ddPCR System is Bio-Rad's Droplet Digital PCR system that is FDA-cleared for use with QXDx BCR-ABL %IS Kit monitoring in the clinical lab. The same system, which includes the QXDx Automated Droplet Generator and QXDx Droplet Reader, provides precise and absolute quantification of target DNA and RNA molecules in a wide variety of research applications.

Features and Benefits

  • Absolute quantification — provides an absolute count of target DNA copies per input without the need for standard curves
  • Highest precision and sensitivity — enriched template concentration in target-positive droplets enabling higher precision and sensitivity than quantitative PCR (qPCR)
  • High throughput — simple and easy-to-use workflow with 96-reaction throughput
  • Removal of PCR efficiency bias — error rates are reduced by removing the reliance on amplification efficiency of qPCR due to high tolerance of PCR inhibitors
  • Automated droplet generator — simplifies the ddPCR workflow and offers flexibility

Research Applications and Uses

  • Copy number variation
  • Rare sequence detection
  • Gene expression analysis
  • Next-generation sequencing (NGS) library quantification
  • Viral load determination
  • Rare mutant detection
  • miRNA analysis
  • GMO detection

Included Components

  • QXDx Automated Droplet Generator
  • QXDx Droplet Reader
  • QXDx Acquisition and Analysis Software
  • QXDx Dual Partitioned Laptop
  • QuantaSoft Software

Related Products

The QXDx AutoDG ddPCR System is used and validated with the following thermal cycler specifications:

  • Accuracy: ±0.2°C
  • Uniformity: ±0.4°C well-to-well within 10 sec
  • Adjustable ramp capability with required ramp rate: up to 2°C/sec
  • Temperature range: 0–100°C

The C1000 Touch Thermal Cycler with 96–Deep Well Reaction Module (1851197) was used during the development and validation efforts of all QXDx products.

Related Categories

Digital PCR

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PX1-PCR-Plate-Sealer-SKU-181-4000-thumb.jpg
(Discontinued) PX1 PCR Plate Sealer
1814000
PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord
17002229-qxdx-autodg-ddpcr-system-tn.jpg
17005351
The FDA-cleared QXDx AutoDG Droplet Digital PCR System includes QXDx Automated Droplet Generator, QXDx Droplet Reader, QXDx Laptop Computer, and QXDx Software. The same FDA-cleared system can be used with the RUO software for probe-based ddPCR applications.

Accessories

Accessories

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1864125
Pkg of 10, pipet tip waste bins for use with the Automated Droplet Generator in the QX200™ AutoDG™ Droplet Digital™ PCR System

Kits & Reagents

Kits & Reagents

Documents

Documents

Number Description Options
12012076
QXDx Operation Manual