One of the most exciting recent developments in genetic engineering is CRISPR-Cas9 (CRISPR). CRISPR derives its name from "clustered regularly interspaced short palindromic repeats," genomic sequences that microbes use to defend themselves against viral attacks. Along with CRISPR associated (Cas) proteins, bacteria use the sequences to recognize and disarm future invading viruses. Scientists have adopted this system for use in genetic engineering.
CRISPR-Cas technology allows scientists to edit genes and manipulate gene expression with a level of ease that was not possible using other methods. Importantly, it also allows researchers to edit genes within living organisms, a fact that supports the use of CRISPR-Cas in a far-reaching range of applications from basic research to the development of novel therapies and other biotechnology products.
The 2020 Nobel Prize for Chemistry was awarded to Emmanuelle Charpentier and Jennifer Doudna for the development of this method for genome editing.
Humans have complex immune systems that involve the coordinated activities of multiple cell types, organs, and signaling systems to recognize and respond to active infections. Prokaryotes — bacteria and archaea — also have a form of adaptive immunity that allows them to recognize and respond to viral infections.
Bacterial Chromosome A CRISPR region within a microbial genome.
Some prokaryotic genomes contain short, palindromic DNA sequences that are repeated many times, with unique "spacer" sequences between the repeats. These "clustered regularly interspaced short palindromic repeats" (CRISPR) are followed by short segments of spacer DNA that match DNA sequences found in bacteriophage genomes (bacteriophage are viruses known to infect bacteria). Groupings of CRISPR-associated (Cas) genes are also found next to CRISPR sequences, and these Cas genes encode enzymes that cut DNA in specific places, like precise molecular scissors.
CRISPR-Cas9 sequences work together to provide an immune response in which a microbe could recognize invading DNA and unleash Cas enzymes to cut and disarm it. CRISPR sequences and the Cas enzymes are the keys to the microbial "adaptive immune response," which involves three phases:
Cutting and Capture — When bacteria are infected by a virus, they use their CRISPR system to cut up the invading viral DNA and insert pieces of it (spacers) into their own genome as a "memory" of the infection
Monitoring — Bacteria transcribe the spacers into RNA, which can form a complex with the Cas9 enzyme. These complexes monitor the cell for any DNA sequence complementary to the RNA
Defense — If matching (viral) DNA is encountered, the spacer RNA-Cas9 complex binds to it and cuts the viral DNA to prevent it from replicating. This halts the viral infection
Using this system, bacteria can collect sequences from many different infecting viruses to create a "library." Since the CRISPR sequence is contained in genomic DNA, it is passed on to each generation, and the library continues to change and adapt to more common threats over time.
Acquire Foreign DNA Sequences
Defend Against Future Infection
The CRISPR-Cas9 microbial defense system. 1. The Cas1-Cas2 enzymes of the microbe recognize and cut out a segment of foreign DNA.2.The Cas1-Cas2 enzymes insert the DNA segment into the CRISPR region of the bacterial genome as a spacer.3.A spacer sequence is transcribed and then linked to a Cas9 protein. 4. Upon reinfection by the same invader, the CRISPR-Cas9 complex can recognize the foreign DNA sequence and cut it to prevent complete reinfection.
CRISPR-Cas9 — A Gene Editing System
The CRISPR-Cas9 system uses modified components of the bacterial CRISPR system to direct target-specific cutting of double-stranded DNA. DNA repair mechanisms then take over to fix the break in a manner that modifies the genetic sequence that has been cut.
Cutting the DNA
Cas9 enzyme (Cas9) — an endonuclease that cuts both strands of DNA at a specific site. Multiple types of Cas enzymes are found in nature, but Cas9 is commonly used in the laboratory
Single guide RNA (sgRNA) — an engineered RNA that forms a complex with Cas9. The sgRNA is a fusion of two regions that occur as separate RNAs in nature:
Guiding region — part of the CRISPR RNA or crRNA in nature, a 20-nucleotide region that is complementary to the target region and defines the target DNA sequence that Cas9 cuts. Scientists customize this sequence for their own targets
Scaffold region — the trans-activating CRISPR RNA or tracrRNA in nature, this region forms a multi-hairpin loop structure (scaffold) that binds in a crevice of the Cas9 protein
Protospacer adjacent motif (PAM) — required for Cas9 function, this sequence motif is immediately downstream of the target sequence. Cas9 recognizes the PAM sequence 5’-NGG, where N can be any nucleotide (A, T, C, or G). When Cas9 binds the PAM, it separates the DNA strands of the adjacent sequence to allow binding of the sgRNA. If the sgRNA is complementary to that sequence, Cas9 cuts the DNA
5 Steps of Cas9 DNA Cleavage
1. Cas9 Binds an sgRNA
Cas9 recognizes and binds the scaffold (tracrRNA) region of a sgRNA. The nucleotide sequence of the scaffold region determines its structure, which is tailored to fit within the Cas9 protein as a key fits into a lock.
2. The Cas9-sgRNA complex binds to a PAM site on the target DNA
Cas9 requires a particular PAM sequence (5’-NGG) to be present directly adjacent to the protospacer sequence. When the Cas9-sgRNA complex recognizes and binds a PAM, it separates the DNA strands of the adjacent sequence to allow binding of the sgRNA.
3. The guiding region of the sgRNA binds to the target DNA sequence
The guiding region of the sgRNA attempts to base-pair with the DNA. If a match is found, the process continues. Otherwise, the complex releases and attempts to bind another PAM and target DNA sequence.
4. Cas9 makes a double-stranded break in the DNA three base pairs upstream of the PAM
5. The complex releases from the DNA
The Cas9-sgRNA complex releases the cut DNA and is ready to repeat the process.
Repairing the Break to Engineer the Change
Researchers can use the cell’s own DNA repair machinery to modify, insert, or delete a nucleotide sequence. The repair can happen in two ways:
Non-homologous end joining (NHEJ) — enzymes reconnect the ends of the double-stranded break back together. This process may randomly insert or delete one or more bases and can cause mutations that can disrupt gene function or expression
Homology directed repair (HDR) — proteins patch the break using donor template DNA. Researchers design the donor template DNA that may include a desired sequence flanked on both sides by "homology arms" that match the sequence upstream and downstream of the cut. A complementary DNA strand is created during the repair
DNA repair via homology directed repair and non-homologous end joining
Applications of CRISPR Technology
With CRISPR, targeted disruption of any gene — in most organisms — is possible. It allows scientists to modify genomic DNA with precision to ensure that no other genes or sequences are unintentionally disrupted. CRISPR technology is easier, faster, and less expensive than other gene-editing techniques and can be used to edit multiple genes at the same time in a single cell. Finally, CRISPR requires the introduction of only one protein (Cas9) and one sgRNA into a cell. Such a powerful technology can be expected to have a vast range of applications.
Medicine
Researchers are looking to CRISPR as a technique for editing out genetic defects that result in sickle cell disease, cystic fibrosis, hemophilia, and muscular dystrophy, and for developing more targeted and effective cancer treatments. One study showed that adult rats engineered to have a genetic form of blindness could be treated using CRISPR gene therapy (Berry et al. 2019). The goal is to someday have patients' diseased cells removed, "fixed" with CRISPR, and then returned to their bodies to treat various conditions or have diseased organs be treated directly with CRISPR. The potential for using CRISPR to change genetic traits in humans has raised serious concerns, about possible unintended effects, as well as ethical questions. The ease of applying CRISPR has caused worry about the potential misuse of the technology. Despite these concerns, CRISPR is revolutionizing many aspects of biotechnology and scientific research.
Agriculture
CRISPR technology is expected to accelerate the development of new, improved crops. The technology has produced crops and livestock with desirable traits such as faster growth, higher nutrient content, and disease resistance. And, since CRISPR technology can modify genes without introducing new genes, CRISPR-modified plants may be subjected to lighter regulations than other genetically modified crops.
Industry
Scientists have used a modified CRISPR-Cas9 system to create a yeast strain to produce lipids and polymers. These molecules could be useful in the development of biofuels, adhesives, and fragrances. Currently, these lipids and polymers are made synthetically from non-renewable petroleum-based materials that are more expensive and could present safety risks.
Public Health
Scientists are experimenting with using CRISPR to engineer "gene drives" to spread specific genes through a population of insect pests that cause them to die or become infertile. This technique is being considered to eradicate mosquitoes carrying human pathogens like malaria parasites or Zika virus.
Use CRISPR-Cas9 gene editing technology for editing the lacZ gene in E. coli. Visualize results with blue-white screening and confirm them by PCR. Deepen your understanding of CRISPR, its promise and risks, and the ethics behind its many applications.
en-usQ58I0DWDLBV5CRISPR-Case Gene Editing ResourcesCRISPR-Cas Gene Editing Teaching Resourcestommy aCRISPR-Cas Gene Editing Teaching ResourcesLearn how CRISPR-Cas technology allows scientists to edit genes and manipulate gene
expression with a level of ease that was not possible using other methods.
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<div class="opt-module crispr-overview opt-module-noborder"><!---<div class="corner-box
corner-box-promo marginbttm-15 linkpointer"><img src="/webroot/web/images/lse/support/CRISPR_GENE-callout_img_160x160.png"
border="0" alt="CRISPR for Gene" style="width: 75px; height: auto;" caption="false"
width="75" height="75" />
<h2>Using CRISPR in your research?</h2>
<p>See <a href="/en-us/applications-technologies/crispr-gene-editing-workflow?ID=PV47ZCRA83CC&WT.mc_id=191204027431">Bio-Rad’s
Solutions for Gene Editing Workflows</a></p>
</div>--->
<p>One of the most exciting recent developments in genetic engineering is CRISPR-Cas9
(CRISPR). CRISPR derives its name from "clustered regularly interspaced short palindromic
repeats," genomic sequences that microbes use to defend themselves against viral attacks.
Along with CRISPR associated (Cas) proteins, bacteria use the sequences to recognize
and disarm future invading viruses. Scientists have adopted this system for use in
genetic engineering.</p>
<p>CRISPR-Cas technology allows scientists to edit genes and manipulate gene expression
with a level of ease that was not possible using other methods. Importantly, it also
allows researchers to edit genes within living organisms, a fact that supports the
use of CRISPR-Cas in a far-reaching range of applications from basic research to the
development of novel therapies and other biotechnology products.</p>
<p>The <a href="https://www.nobelprize.org/prizes/chemistry/2020/press-release/" target="_blank"
rel="noopener noreferrer">2020 Nobel Prize for Chemistry</a> was awarded to Emmanuelle
Charpentier and Jennifer Doudna <span>for the development of this method for
genome editing.</span></p>
<p>This page contains background information about the CRISPR technology as well as
other resources and activities. Please also watch our video, <a href="https://youtu.be/tNocAc5TKvw"
target="_blank" rel="noopener noreferrer">CRISPR Explained: Gene Editing History,
Technology, and Applications</a>.</p>
</div>
<div><a name="immune"></a></div>
<div class="opt-module bg-edf6e4 opt-module-noborder">
<h2 class="nextp">CRISPR-Cas — A Microbial Immune System</h2>
<p>Humans have complex immune systems that involve the coordinated activities of multiple
cell types, organs, and signaling systems to recognize and respond to active infections.
Prokaryotes — bacteria and archaea — also have a form of adaptive immunity
that allows them to recognize and respond to viral infections.</p>
<div class="right width45"><img class="top-margin" src="/webroot/web/images/lse/solutions/applications/preparing_for_class/classroom_resources/crispr-bacterial-chromosome.png"
alt="" />
<p class="caption topmargin-1em"><strong>Bacterial Chromosome</strong> A CRISPR region
within a microbial genome.</p>
</div>
<p>Some prokaryotic genomes contain short, palindromic DNA sequences that are repeated
many times, with unique "spacer" sequences between the repeats. These "clustered regularly
interspaced short palindromic repeats" (CRISPR) are followed by short segments of
spacer DNA that match DNA sequences found in bacteriophage genomes (bacteriophage
are viruses known to infect bacteria). Groupings of CRISPR-associated (Cas) genes
are also found next to CRISPR sequences, and these Cas genes encode enzymes that cut
DNA in specific places, like precise molecular scissors.</p>
<p class="top-margin">CRISPR-Cas9 sequences work together to provide an immune response
in which a microbe could recognize invading DNA and unleash Cas enzymes to cut and
disarm it. CRISPR sequences and the Cas enzymes are the keys to the microbial "adaptive
immune response," which involves three phases:</p>
<ul>
<li><strong>Cutting and Capture</strong> — When bacteria are infected by a virus,
they use their CRISPR system to cut up the invading viral DNA and insert pieces of
it (spacers) into their own genome as a "memory" of the infection</li>
<li><strong>Monitoring</strong> — Bacteria transcribe the spacers into RNA,
which can form a complex with the Cas9 enzyme. These complexes monitor the cell for
any DNA sequence complementary to the RNA</li>
<li><strong>Defense</strong> — If matching (viral) DNA is encountered, the spacer
RNA-Cas9 complex binds to it and cuts the viral DNA to prevent it from replicating.
This halts the viral infection</li>
</ul>
<p>Using this system, bacteria can collect sequences from many different infecting
viruses to create a "library." Since the CRISPR sequence is contained in genomic DNA,
it is passed on to each generation, and the library continues to change and adapt
to more common threats over time.</p>
<ul class="list-set list-two-col top-margin">
<li class="no-bttm-pddng"><img src="/webroot/web/images/lse/solutions/applications/preparing_for_class/classroom_resources/microbial-defense-system-aquire.jpg"
alt="Illustration of how CRISPR-Cas9 acquires foreign DNA sequences" /><center><strong>Acquire
Foreign DNA Sequences</strong></center></li>
<li class="no-bttm-pddng"><img src="/webroot/web/images/lse/solutions/applications/preparing_for_class/classroom_resources/microbial-defense-system-defend.jpg"
alt="Illustration of how CRISPR-Cas9 defends against future infection" /><center><strong>Defend
Against Future Infection</strong></center></li>
</ul>
<br clear="all" />
<p class="caption topmargin-1em"><strong>The CRISPR-Cas9 microbial defense system.
1.</strong> <span style="font-weight: 400;"> The</span><span style="font-weight:
400;"> Cas1-Cas2 enzymes of the microbe recognize </span><span style="font-weight:
400;">and cut out a segment of foreign DNA.</span> <strong>2.</strong> <span
style="font-weight: 400;">The Cas1-Cas2 enzymes insert the DNA segment into the CRISPR
region </span><span style="font-weight: 400;">of the bacterial genome</span><span
style="font-weight: 400;"> as a spacer.</span> <strong>3.</strong> <span style="font-weight:
400;">A spacer sequence is transcribed and then linked to a Cas9 protein. </span><strong>4.</strong>
Upon reinfection by the same invader, the CRISPR-Cas9 complex can recognize the foreign
DNA sequence and cut it to prevent complete <span style="font-weight: 400;">reinfection</span>.</p>
</div>
<div><a name="editing"></a></div>
<div class="opt-module">
<h2 class="nextp">CRISPR-Cas9 — A Gene Editing System</h2>
<p>The CRISPR-Cas9 system uses modified components of the bacterial CRISPR system
to direct target-specific cutting of double-stranded DNA. DNA repair mechanisms then
take over to fix the break in a manner that modifies the genetic sequence that has
been cut.</p>
<h3 class="opt-subhead30">Cutting the DNA</h3>
<ul>
<li><strong>Cas9 enzyme (Cas9)</strong> — an endonuclease that cuts both strands
of DNA at a specific site. Multiple types of Cas enzymes are found in nature, but
Cas9 is commonly used in the laboratory</li>
<li><strong>Single guide RNA (sgRNA)</strong> — an engineered RNA that forms
a complex with Cas9. The sgRNA is a fusion of two regions that occur as separate RNAs
in nature:
<ul class="topmargin-1em">
<li>Guiding region — part of the CRISPR RNA or crRNA in nature, a 20-nucleotide
region that is complementary to the target region and defines the target DNA sequence
that Cas9 cuts. Scientists customize this sequence for their own targets</li>
<li>Scaffold region — the trans-activating CRISPR RNA or tracrRNA in nature,
this region forms a multi-hairpin loop structure (scaffold) that binds in a crevice
of the Cas9 protein</li>
</ul>
</li>
<li><strong>Protospacer adjacent motif (PAM)</strong> — required for Cas9 function,
this sequence motif is immediately downstream of the target sequence. Cas9 recognizes
the PAM sequence 5’-NGG, where N can be any nucleotide (A, T, C, or G). When
Cas9 binds the PAM, it separates the DNA strands of the adjacent sequence to allow
binding of the sgRNA. If the sgRNA is complementary to that sequence, Cas9 cuts the
DNA</li>
</ul>
<div class="corner-box top-margin">
<h3 class="opt-subhead30">5 Steps of Cas9 DNA Cleavage</h3>
<ul class="slider slider-fade mid-align">
<li>
<div><img src="/webroot/web/images/lse/solutions/applications/preparing_for_class/classroom_resources/cas9-dna-cleavage-wf-step1.gif"
alt="" /></div>
<div>
<h3>1. Cas9 Binds an sgRNA</h3>
<p>Cas9 recognizes and binds the scaffold (tracrRNA) region of a sgRNA. The nucleotide
sequence of the scaffold region determines its structure, which is tailored to fit
within the Cas9 protein as a key fits into a lock.</p>
</div>
</li>
<li>
<div><img src="/webroot/web/images/lse/solutions/applications/preparing_for_class/classroom_resources/cas9-dna-cleavage-wf-step2.gif"
alt="" /></div>
<div>
<h3>2. The Cas9-sgRNA complex binds to a PAM site on the target DNA</h3>
<p>Cas9 requires a particular PAM sequence (5’-NGG) to be present directly adjacent
to the protospacer sequence. When the Cas9-sgRNA complex recognizes and binds a PAM,
it separates the DNA strands of the adjacent sequence to allow binding of the sgRNA.</p>
</div>
</li>
<li>
<div><img src="/webroot/web/images/lse/solutions/applications/preparing_for_class/classroom_resources/cas9-dna-cleavage-wf-step3.gif"
alt="" /></div>
<div>
<h3>3. The guiding region of the sgRNA binds to the target DNA sequence</h3>
<p>The guiding region of the sgRNA attempts to base-pair with the DNA. If a match
is found, the process continues. Otherwise, the complex releases and attempts to bind
another PAM and target DNA sequence.</p>
</div>
</li>
<li>
<div><img src="/webroot/web/images/lse/solutions/applications/preparing_for_class/classroom_resources/cas9-dna-cleavage-wf-step4.gif"
alt="" /></div>
<div>
<h3>4. Cas9 makes a double-stranded break in the DNA three base pairs upstream of
the PAM</h3>
</div>
</li>
<li>
<div><img src="/webroot/web/images/lse/solutions/applications/preparing_for_class/classroom_resources/cas9-dna-cleavage-wf-step5.gif"
alt="" /></div>
<div>
<h3>5. The complex releases from the DNA</h3>
<p>The Cas9-sgRNA complex releases the cut DNA and is ready to repeat the process.</p>
</div>
</li>
</ul>
</div>
<br clear="all" />
<h3 class="opt-subhead30">Repairing the Break to Engineer the Change</h3>
<p>Researchers can use the cell’s own DNA repair machinery to modify, insert,
or delete a nucleotide sequence. The repair can happen in two ways:</p>
<ul>
<li><strong>Non-homologous end joining (NHEJ)</strong> — enzymes reconnect the
ends of the double-stranded break back together. This process may randomly insert
or delete one or more bases and can cause mutations that can disrupt gene function
or expression</li>
<li><strong>Homology directed repair (HDR)</strong> — proteins patch the break
using donor template DNA. Researchers design the donor template DNA that may include
a desired sequence flanked on both sides by "homology arms" that match the sequence
upstream and downstream of the cut. A complementary DNA strand is created during the
repair</li>
</ul>
<center><img class="top-margin" src="/webroot/web/images/lse/solutions/applications/preparing_for_class/classroom_resources/crispr-dna-repair.gif"
alt="Illustration showing DNA repair via homology directed repair and non-homologous
end joining" />
<p class="caption topmargin-1em"><strong>DNA repair via homology directed repair and
non-homologous end joining</strong></p>
</center></div>
<div><a name="applications"></a></div>
<div class="opt-module opt-module-noborder bg-283148 crispr-applications white"><img
src="/webroot/web/images/1x1.gif" height="230px" width="300px" class="right" alt=""
/>
<h2 class="nextp">Applications of CRISPR Technology</h2>
<p>With CRISPR, targeted disruption of any gene — in most organisms —
is possible. It allows scientists to modify genomic DNA with precision to ensure that
no other genes or sequences are unintentionally disrupted. CRISPR technology is easier,
faster, and less expensive than other gene-editing techniques and can be used to edit
multiple genes at the same time in a single cell. Finally, CRISPR requires the introduction
of only one protein (Cas9) and one sgRNA into a cell. Such a powerful technology can
be expected to have a vast range of applications.</p>
<h3>Medicine</h3>
<p>Researchers are looking to CRISPR as a technique for editing out genetic defects
that result in sickle cell disease, cystic fibrosis, hemophilia, and muscular dystrophy,
and for developing more targeted and effective cancer treatments. One study showed
that adult rats engineered to have a genetic form of blindness could be treated using
CRISPR gene therapy (Berry et al. 2019). The goal is to someday have patients' diseased
cells removed, "fixed" with CRISPR, and then returned to their bodies to treat various
conditions or have diseased organs be treated directly with CRISPR. The potential
for using CRISPR to change genetic traits in humans has raised serious concerns, about
possible unintended effects, as well as ethical questions. The ease of applying CRISPR
has caused worry about the potential misuse of the technology. Despite these concerns,
CRISPR is revolutionizing many aspects of biotechnology and scientific research.</p>
<h3>Agriculture</h3>
<p>CRISPR technology is expected to accelerate the development of new, improved crops.
The technology has produced crops and livestock with desirable traits such as faster
growth, higher nutrient content, and disease resistance. And, since CRISPR technology
can modify genes without introducing new genes, CRISPR-modified plants may be subjected
to lighter regulations than other genetically modified crops.</p>
<h3>Industry</h3>
<p>Scientists have used a modified CRISPR-Cas9 system to create a yeast strain to
produce lipids and polymers. These molecules could be useful in the development of
biofuels, adhesives, and fragrances. Currently, these lipids and polymers are made
synthetically from non-renewable petroleum-based materials that are more expensive
and could present safety risks.</p>
<h3>Public Health</h3>
<p>Scientists are experimenting with using CRISPR to engineer "gene drives" to spread
specific genes through a population of insect pests that cause them to die or become
infertile. This technique is being considered to eradicate mosquitoes carrying human
pathogens like malaria parasites or Zika virus.</p>
<p><strong>Using CRISPR in your research?</strong> See <a href="/en-us/applications-technologies/crispr-gene-editing-workflow?ID=PV47ZCRA83CC&WT.mc_id=191204027431"><span
class="orange">Bio-Rad’s Solutions for Gene Editing Workflows</span></a></p>
</div>
<!--Student Activity START-->
<div><a name="activities"></a></div>
<div class="opt-module nobttm-brdr">
<div class="corner-box width35 right bg-white linkpointer-blank"><a href="https://www.youtube.com/playlist?list=PL7_N-H8d6RiOTKL7AIOzP69C8wYWKYOi_"
target="_blank" aria-label="Vist our Life Science Education YouTube Channel" rel="noopener
noreferrer" class=""><img src="/webroot/web/images/lse/solutions/applications/preparing_for_class/classroom_resources/crispr-playlist-3col.jpg"
alt="CRISPR Gene Editing" width="280px" /></a>
<h3><a href="https://www.youtube.com/playlist?list=PL7_N-H8d6RiOTKL7AIOzP69C8wYWKYOi_"
target="_blank" rel="noopener noreferrer" class="new-target-image" title="Link opens
in new window">YouTube CRISPR Gene Editing Playlist</a></h3>
<p>Have your students gain perspectives about CRISPR from some of the industry’s
leading experts.</p>
</div>
<h2>Classroom Activities and Resources</h2>
<!--h3 class="opt-subhead opt-subhead-gap">Student Activities</h3-->
<h3><a href="/webroot/web/pdf/lse/literature/CRISPR Cas 9 Paper Model Activity.pdf"
target="_blank" rel="noopener noreferrer" class="no-target-style">CRISPR Paper Model
Activity</a> <span class="filesize">(PDF 675 KB)</span></h3>
<p>Have your students use this CRISPR paper model activity at home to learn about
the CRISPR-Cas9 system.</p>
<h3><a href="/webroot/web/pdf/lse/literature/CRISPR Bioinformatics Capstone Activity.pdf"
target="_blank" rel="noopener noreferrer" class="no-target-style">Bioinformatics Activity</a>
<span class="filesize">(PDF 6.1 MB)</span></h3>
<p>Students can use this activity to design Cas9 target sites in the human genome
and determine risk for off-target effect.</p>
<h3><a href="/webroot/web/pdf/lse/misc/Out_of_the_Blue_CRISPR_Kit_Classroom_Presentation.pptx"
target="_blank" rel="noopener noreferrer" class="no-target-style">PowerPoint Presentation
for Classroom Use </a><span class="filesize">(PPT 14.9 MB)</span></h3>
<p>Use and modify this slide deck to help guide your students through the CRISPR-Cas9
gene editing lab activity.</p>
</div>
<!--Student Activity END-->
<div><a name="references"></a></div>
<div class="opt-module opt-module-noborder bg-eaf4f6">
<h2>Gene Editing and CRISPR References</h2>
<ol>
<li>Berry MH et al. (2019) <a href="https://www.nature.com/articles/s41467-019-09124-x"
target="_blank" rel="noopener noreferrer">Restoration of high-sensitivity and adapting
vision with a cone opsin</a>. Nature Communications 10, 1221.</li>
<li>Carroll D (2017) <a href="https://pdfs.semanticscholar.org/9e4c/c65133f84a24587082e86ffe58784bbdb476.pdf"
target="_blank" rel="noopener noreferrer">Genome editing: past, present, and future</a>.
Yale J Biol Med 90, 653–659.</li>
<li>Cong L et al. (2013) <a href="https://science.sciencemag.org/content/339/6121/819"
target="_blank" rel="noopener noreferrer">Multiplex genome engineering using CRISPR/Cas
systems</a>. Science 339, 819–823.</li>
<li>Cribbs A et al. (2017) <a href="https://pdfs.semanticscholar.org/b26c/08d31cf3a632f97c8067882291f38ed30cda.pdf?_ga=2.200093741.2018895055.1573006267-1029194739.1573006267"
target="_blank" rel="noopener noreferrer">Science and bioethics of CRISPR-Cas9 gene
editing: an analysis towards separating facts and fiction</a>. Yale J Biol Med 90,
625–634.</li>
<li>Jinek M et al. (2012) <a href="https://science.sciencemag.org/content/337/6096/816"
target="_blank" rel="noopener noreferrer">A programmable dual RNA-guided DNA endonuclease
in adaptive bacterial immunity</a>. Science 337, 816–821.</li>
<li>Park A (2016) <a href="https://time.com/4379503/crispr-scientists-edit-dna/" target="_blank"
rel="noopener noreferrer">A new technique that lets scientists edit DNA is transforming
science — and raising difficult questions</a>. Time 43–48.</li>
<li>Thurtle‐Schmidt D and Lo T (2018) <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5901406/"
target="_blank" rel="noopener noreferrer">Molecular biology at the cutting edge: A
review on CRISPR/CAS9 gene editing for undergraduates.</a> Biochem Mol Biol Educ 46,
195-205.</li>
</ol>
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<h2 class="nextp">CRISPR for the Classroom</h2>
<a onmousedown="dcsMultiTrack('DCS.dcsqry','?tgp_pcp_link_group=CRISPR for the Classroom&tgp_pcp_link=Learn
More','DCSext.tgp_pcp_link_group','','DCSext.tgp_pcp_link','','WT.dl','99999');" href="/en-us/product/out-blue-crispr-kit-genotyping-extension?ID=Q0JGD4E08O1Y"
aria-label="Learn more about our Out of the Blue CRISPR Kit and Genotyping Extension">
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alt="Package contents of Out of the Blue CRISPR Kit and Genotyping Extension" class="right"
/></a>
<h3>Out of the Blue CRISPR Kits</h3>
<p>Use CRISPR-Cas9 gene editing technology for editing the <em>lacZ</em> gene in <em
class="forcebreaks">E. coli</em>. Visualize results with blue-white screening and
confirm them by PCR. Deepen your understanding of CRISPR, its promise and risks, and
the ethics behind its many applications.</p>
<p><a class="bttn-block" onmousedown="dcsMultiTrack('DCS.dcsqry','?tgp_pcp_link_group=CRISPR
for the Classroom&tgp_pcp_link=Learn More','DCSext.tgp_pcp_link_group','','DCSext.tgp_pcp_link','','WT.dl','99999');"
href="/en-us/product/out-blue-crispr-kit-genotyping-extension?ID=Q0JGD4E08O1Y" aria-label="Learn
more about our Out of the Blue CRISPR Kit and Genotyping Extension"><span class="bttn-orange">Learn
More</span></a></p>
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1Classroom Resources
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