Out of the Blue CRISPR and Genotyping Extension Kits

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CRISPR Gene Editing and Genotyping Lab Activity Kit from Bio-Rad

Overview

Lab Preparation Checklist
Lab Preparation Checklist

Out of the Blue CRISPR Kit (sufficient materials for 8 student workstations with 2–4 students per workstation)

Kit Refill Pack (12012620EDU) — store at -20°C
E. coli HB101-pBRKan, lyophilized  
Donor template DNA plasmid (pLZDonor)  
Donor template DNA and guide RNA plasmid (pLZDonorGuide)  
KIX Mix  
Spectinomycin powder  
Arabinose, lyophilized  
Sterile transformation solution  

Store at room temperature
LB nutrient agar powder  
LB broth capsule  
Sterile inoculation loops, 80  
Sterile Petri dishes, 60  
Sterile 2.0 ml microcentrifuge tubes, 90  
Quick Start Guide  
Instructor Answer Guide  



Required Materials (not included in this kit)

100–1,000 µl adjustable-volume micropipet and tips 1–8
20–200 μl adjustable-volume micropipet and tips 4–8
20–20 μl adjustable-volume micropipet and tips 4–8
Balance with a range of 1-10 g 1
Autoclave or microwave over 1
Thermometer (0–60°C) 1
Temperature-controlled dry bath or water bath 1
Foam floats, if using a water bath 8
Erlenmeyer flask, 1L 2
Autoclavable bottle with cap, 150–250 ml 1
Graduated cylinder, 500 ml  
Deionized or distilled water  
Crushed ice and containers (e.g., ice bucket or styrofoam cup) 8
Scissors 8
Permanent marking pens 8
Aluminum foil  
Household bleach, 10% solution  
Laboratory tape  


Recommended Materials (not included in this kit)

Incubation oven or shaking incubator with dish shelf 1
Pipet controller and sterile serological pipets 1
Activity Flowchart
Out of the Blue CRISPR Kit Activity Flowchart
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The Out of the Blue CRISPR Kit is now available for purchase.
The Out of the Blue Genotyping Extension will be available by April 30, 2020.

CRISPR for Gene

Using CRISPR in your research?
See Bio-Rad’s Solutions for Gene Editing Workflows

Real CRISPR Gene Editing - in Your Classroom

Perform true CRISPR gene editing in your laboratory class!
CRISPR gene editing is revolutionizing scientific research. Now your students can do real CRISPR-Cas9 gene editing in the classroom using Bio-Rad's educational CRISPR gene editing kits.

  • Out of the Blue CRISPR Kit — apply CRISPR-Cas9 technology to disrupt the lacZ gene in E. coli and produce a phenotype that can be readily visualized through blue-white screening
  • Out of the Blue CRISPR Genotyping Extension — (optional) confirm the edit at the molecular level using PCR and agarose gel electrophoresis

Features and Benefits

  • Hands-on, true CRISPR gene editing — students get direct experience with this powerful technology
  • Visible phenotype — results can be observed with the naked eye in as little as 22 hr
  • Safe — familiar and common reagents and non-pathogenic, non-biofilm-forming bacteria generate a phenotype that is common to molecular biology
  • Accessible — does not require specialized equipment or techniques
  • Comprehensive, supportive curriculum — includes background about the components of a CRISPR-Cas9 system, the science — and math — behind its accuracy and flexibility, and the guided bioinformatic analysis of CRISPR targets students design themselves

Activity Overview

  • 1

    Modeling the components of the CRISPR-Cas9 System

    Use a paper model to understand the components of the CRISPR-Cas9 system and the math behind its accuracy

  • 2

    lacZ CRISPR Gene Editing Laboratory

    Use CRISPR-Cas9 to edit the lacZ gene in E. coli

  • 3

    Genotyping Laboratory (Optional)*

    Use PCR and gel electrophoresis to confirm the edit in transformants

  • 4

    Identification and Bioinformatic Analysis of Cas9 Target Sites in Human Disease

    Use bioinformatics to design Cas9 target sites in the human genome and determine risk for off-target effect

*Requires purchase of the Out of the Blue Genotyping Extension

  • CRISPR Title Image

    Learn More About CRISPR Gene Editing

    Appreciating the unique capabilities and limitations of CRISPR enriches the conversation around its applications and ethics in research, agriculture, and medicine. Additional resources can be found below.

    CRISPR Gene Editing Resources »

  • Teaching CRISPR Infographic

    What Will You Teach with CRISPR?

    With applications across medicine, agriculture, and beyond, what is the true promise of CRISPR? Should its use be regulated? What are the laws, ethical considerations, and practical limitations to this technology?

    Download the infographic

What Will You Need to Do CRISPR?

The Out of the Blue CRISPR Kit provides all the plasmids, bacterial strains, culture reagents, and plastics to perform CRISPR gene editing in the classroom. Each kit supports 32 students or 8 student workstations; reagent refill packs are also available.

See the Lab Preparation Checklist for information about all required recommended supplies.

For the Out of the Blue CRISPR Kit


For the Out of the Blue Genotyping Extension

Do You Qualify for Bio-Rad Educational Discount?

If you are a life science educator, you may qualify for a special discounted price (EDU price) on Bio-Rad’s Life Science Education kits, equipment, and reagents. The discount applies to products used for educational purposes only in K–12 and undergraduate classrooms.

Contact Customer Care to request an account number.

Out of the Blue CRISPR Kit
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
E. coli HB101-pBRKan, lyophilized
Donor template DNA (pLZDonor)
Donor template DNA and guide RNA (pLZDonorGuide)
KIX Mix
Spectinomycin powder
Arabinose, lyophilized
Sterile transformation solution
LB nutrient agar powder
LB broth capsule, makes 50 ml LB broth
Sterile inoculation loops 80
Sterile Petri dishes 60
Sterile 2.0 ml microcentrifuge tubes 90
Easy Start Guide
Instructor Answer Guide
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Out of the Blue CRISPR Kit

12012608EDU
CRISPR-Cas9 gene editing kit, includes Refill Pack (12012620EDU) materials, LB agar and broth, Petri dishes, inoculation loops, and microtubes. For educational use only — not for self-administration.

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Out of the Blue CRISPR Kit Refill Pack

12012620EDU
CRISPR-Cas9 gene editing kit refill, includes bacteria, plasmids, KIX Mix, spectinomycin, arabinose, and transformation solution. For educational use only — not for self-administration.

12012620EDU templatedata/internet/product_SKU/data/LSE/CRISPR-Gene-Editing-Kits/12012620EDU_1578965482.xml 12012620EDU 01/14/20 01:25 AM en_US true internet/product_SKU 2020-02-26 22:59:50 /default/main/bio-rad/STAGING AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,VN,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE templatedata/internet/product_SKU/data/LSE/CRISPR-Gene-Editing-Kits/12012620EDU_1578965482.xml /default/main/bio-rad/WORKAREA/default k41k9ei4 2020-01-14 01:31:23 true k41k9ei4 templatedata/internet/product_SKU/data/LSE/CRISPR-Gene-Editing-Kits/12012620EDU_1578965482.xml LSE N /default/main/bio-rad en 01/13/37 02:30 PM 1579218029969 2020-02-26 22:59:50 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/CRISPR-Gene-Editing-Kits/12012620EDU_1578965482.xml 12012620edu-out-blue-crispr-kit-refill-pack 1 12012620EDU Out of the Blue CRISPR Kit Refill Pack Out of the Blue CRISPR Kit Refill Pack CRISPR-Cas9 gene editing kit refill, includes bacteria, plasmids, KIX Mix, spectinomycin, arabinose, and transformation solution. For educational use only &mdash; not for self-administration. /webroot/web/images/lse/products/crispr-gene-editing-kits/sku_thumb/global/12012620EDU_sku_thumb.jpg /webroot/web/images/lse/products/crispr-gene-editing-kits/sku_view/global/12012620EDU_sku_view.jpg preview TW Out of the Blue CRISPR Kit Refill Pack CRISPR-Cas9 gene editing kit refill, includes bacteria, plasmids, KIX Mix, spectinomycin, arabinose, and transformation solution. For educational use only &mdash; not for self-administration. 12012620EDU, CRISPR, CRISPR Kit, Out of the Blue, CRISPR-Cas9, CRISPR gene editing, gene editing, CRISPR refill, CRISPR kit refills, CRISPR kit refill 01/14/20 01:25 AM 01/13/37 02:30 PM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,VN,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE LSE /LSE/CRISPR-Gene-Editing-Kits N 0 <p>The Out of the Blue CRISPR Kit Refill Pack contains consumable reagents for use with Out of the Blue CRISPR kit (12012608EDU).</p> <p>See the <a href="/en-us/product/out-blue-crispr-kit-genotyping-extension?ID=Q0JGD4E08O1Y">product page</a> for more information.</p> <h2 class="descHeader">Refill Contents</h2> <p>Each kit contains materials for 8 student workstations (2&ndash;4 students per workstation):</p> <p>Store at -20&deg;</p> <ul> <li><i>E. coli</i> HB101-pBRKan, lyophilized</li> <li>Donor template DNA plasmid (pLZDonor)</li> <li>Donor template DNA and guide RNA plasmid (pLZDonorGuide)</li> <li>KIX Mix</li> <li>Spectinomycin powder</li> <li>Arabinose, lyophilized</li> <li>Sterile transformation solution</li> </ul> <br /> <p>We've gone green! Download the <a href="/en-us/product/out-blue-crispr-kit-genotyping-extension?ID=Q0JGD4E08O1Y#fragment-doc">student and instructor manuals</a>.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Out of the Blue CRISPR Kit (<a href="/en-us/sku/12012608edu-out-blue-crispr-kit?ID=12012608EDU">12012608EDU</a>)</li> </ul> 1 0 12012608EDU templatedata/internet/product_SKU/data/LSE/CRISPR-Gene-Editing-Kits/12012608EDU_1578962393.xml false $248.75 true $ %24199.00 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12012620EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12012620EDU&CartQuote=QT&quantity= 1711 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12012620EDU&AddToDefaultWishList=true&quantity= 41106300 248.75 12012620EDU
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Number Description Options
7289 What will you teach with CRISPR? Infographic Click to download
10000122375 Instructor Guide, Out of the Blue CRISPR Kit Click to download
10000122376 Student Guide, Out of the Blue CRISPR Kit Click to download

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