Out of the Blue CRISPR and Genotyping Extension Kits

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Overview

Use CRISPR-Cas9 gene editing to edit the lacZ gene in E. coli. Visualize results with blue-white screening and confirm them by PCR. Deepen your understanding of CRISPR, its promise and risks, and the ethics behind its many applications.

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Description

Description

CRISPR for Gene

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See Bio-Rad’s Solutions for Gene Editing Workflows

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Real CRISPR Gene Editing - in Your Classroom

Perform true CRISPR gene editing in your laboratory class!
CRISPR gene editing is revolutionizing scientific research. Now your students can do real CRISPR-Cas9 gene editing in the classroom using Bio-Rad's educational CRISPR gene editing kits.

  • Out of the Blue CRISPR Kit — apply CRISPR-Cas9 technology to disrupt the lacZ gene in E. coli and produce a phenotype that can be readily visualized through blue-white screening
  • Out of the Blue CRISPR Genotyping Extension — (optional) confirm the edit at the molecular level using PCR and agarose gel electrophoresis

The Out of the Blue CRISPR and Genotyping Extension Kits earned the 2020 Science Educators’ Best of Show award for “Best New Technology Innovation for Science”  from the National Science Teaching Association (NSTA).

Features and Benefits

  • Hands-on, true CRISPR gene editing — students get direct experience with this powerful technology
  • Visible phenotype — results can be observed with the naked eye in as little as 22 hr
  • Safe — familiar and common reagents and non-pathogenic, non-biofilm-forming bacteria generate a phenotype that is common to molecular biology
  • Accessible — does not require specialized equipment or techniques
  • Comprehensive, supportive curriculum — includes background about the components of a CRISPR-Cas9 system, the science — and math — behind its accuracy and flexibility, and the guided bioinformatic analysis of CRISPR targets students design themselves

Activity Overview

  • 1

    Modeling the components of the CRISPR-Cas9 System

    Use a paper model to understand the components of the CRISPR-Cas9 system and the math behind its accuracy

  • 2

    lacZ CRISPR Gene Editing Laboratory

    Use CRISPR-Cas9 to edit the lacZ gene in E. coli

  • 3

    Genotyping Laboratory (Optional)*

    Use PCR and gel electrophoresis to confirm the edit in transformants

  • 4

    Identification and Bioinformatic Analysis of Cas9 Target Sites in Human Disease

    Use bioinformatics to design Cas9 target sites in the human genome and determine risk for off-target effect

*Requires purchase of the Out of the Blue Genotyping Extension

  • CRISPR Title Image

    Learn More About CRISPR Gene Editing

    Appreciating the unique capabilities and limitations of CRISPR enriches the conversation around its applications and ethics in research, agriculture, and medicine. Additional resources can be found below.

    CRISPR Gene Editing Resources »

  • Teaching CRISPR Infographic

    Use our PowerPoint Presentation

    Use this student-facing slide deck, modifying it as needed, to help guide your students through the CRISPR-Cas9 gene editing lab activity.

    Download the presentation

What Will You Need to Do CRISPR?

The Out of the Blue CRISPR Kit provides all the plasmids, bacterial strains, culture reagents, and plastics to perform CRISPR gene editing in the classroom. Each kit supports 32 students or 8 student workstations; reagent refill packs are also available.

See the Lab Preparation Checklist for information about all required recommended supplies.

For the Out of the Blue CRISPR Kit


For the Out of the Blue Genotyping Extension


Do You Qualify for Bio-Rad Educational Discount?

If you are a life science educator, you may qualify for a special discounted price (EDU price) on Bio-Rad’s Life Science Education kits, equipment, and reagents. The discount applies to products used for educational purposes only in K–12 and undergraduate classrooms.

Contact Customer Care to request an account number.

Specifications

Specifications

Out of the Blue CRISPR Kit
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
E. coli HB101-pBRKan, lyophilized
1
Donor template DNA (pLZDonor)
1
Donor template DNA and guide RNA (pLZDonorGuide)
1
KIX mix
1
Spectinomycin powder
1
Arabinose, lyophilized
1
Sterile transformation solution
1
LB nutrient agar powder
1
LB broth capsule, makes 50 ml LB broth
1
Sterile inoculation loops
80
Sterile Petri dishes
60
Sterile 2.0 ml microcentrifuge tubes
90
Easy Start Guide
1
Instructor Answer Guide
1
Out of the Blue Genotyping Ext.
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
Out of the Blue primer mix (50x)
1
Out of the Blue PCR positive control DNA
1
PCR master mix (Taq DNA polymerase, dNTPs, buffers), 1.2 ml
1
PCR molecular weight ruler
1
Orange G Loading Dye
1
InstaGene matrix, 20 ml
1
PCR tubes, 0.2 ml
100
Microentrifuge tubes, 1.5 ml with caps
60
Conical tubes, 1.5 ml, sterile
50
Instructor Answer Guide
1

Ordering

Ordering

items

Use the filters below to refine results!

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17006081EDU
CRISPR-Cas9 gene editing kit (12012608EDU) and genotyping extension kit (120126078EDU) package. Requires electrophoresis reagents, sold separately. For educational use only — not for self-administration.
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12012608EDU
CRISPR-Cas9 gene editing kit, includes Refill Pack (12012620EDU) materials, LB agar and broth, Petri dishes, inoculation loops, and microtubes. For educational use only — not for self-administration.
12012607EDU_tn.jpg
12012607EDU
CRISPR-Cas9 genotyping extension kit, includes Out of the Blue Extension Refill Pack materials (12012708EDU), InstaGene matrix, and PCR, micro, and conical tubes. Requires Out of the Blue CRISPR Kit (12012608EDU) and electrophoresis reagents, sold separately. For educational use only.
17006285EDU_tn.jpg
17006285EDU
CRISPR-Cas9 gene editing kit (12012608EDU) and genotyping extension kit (120126078EDU) package with agarose powder, UView 6x loading dye and stain, and buffer. For educational use only — not for self-administration.
17006286EDU_tn.jpg
17006286EDU
Out of the Blue CRISPR and Genotyping Extension Kits plus Small Fast Blast DNA Electrophoresis Pack
17006284EDU_tn.jpg
17006284EDU
CRISPR-Cas9 genotyping extension kit (120126078EDU) with agarose powder, Fast Blast DNA stain, and buffer. Requires Out of the Blue CRISPR Kit (12012608EDU), sold separately. For educational use only.
17006070EDU_tn.jpg
17006070EDU
CRISPR-Cas9 genotyping extension kit (120126078EDU) with agarose powder, Fast Blast DNA stain, and buffer. Requires Out of the Blue CRISPR Kit (12012608EDU), sold separately. For educational use only.

Refills

Refills

12012620EDU_sku_thumb.jpg
12012620EDU
CRISPR-Cas9 gene editing kit refill, includes bacteria, plasmids, KIX Mix, spectinomycin, arabinose, and transformation solution. For educational use only — not for self-administration.
12012708EDU_tn.jpg
12012708EDU
CRISPR-Cas9 genotyping extension refill, includes primers, positive control DNA, master mix, molecular weight ruler, and loading dye. Requires Out of the Blue CRISPR Kit (12012608EDU) and electrophoresis reagents, sold separately. For educational use only.
small-uview-dna-electrophoresis-reagents-pack-1660462edu-tn.jpg
1660462EDU
Agarose gel preparation kit,makes 16–48 mini gels, includes agarose powder, UView 6x Loading Dye and Stain, buffer; education use only
166-0450EDU_tn.jpg
1660450EDU
Agarose gel preparation kit,makes 16–48 mini gels, includes agarose powder, Fast Blast DNA Stain, buffer; education use only

Documents

Documents

Number Description Options
10000122375
Instructor Guide, Out of the Blue CRISPR Kit
10000122376
Student Guide, Out of the Blue CRISPR Kit
10000122387
Instructor Guide, Out of the Blue Genotyping Extension
10000122388
Student Guide, Out of the Blue Genotyping Extension
7289
What will you teach with CRISPR? (Infographic)