Out of the Blue CRISPR and Genotyping Extension Kits
Use CRISPR-Cas9 gene editing to edit the lacZ gene in E. coli. Visualize results with blue-white screening and confirm them by PCR. Deepen your understanding of CRISPR, its promise and risks, and the ethics behind its many applications.
Overview
Teach Real CRISPR Gene Editing in Your Classroom
Bring real CRISPR-Cas9 gene editing into your classroom with the Out of the Blue CRISPR Kit and Genotyping Extension.
- Out of the Blue CRISPR Kit — use CRISPR-Cas9 technology to introduce a stop codon in the lacZ gene in the E. coli chromosome, then see results easily with blue-white screening!
- Out of the Blue CRISPR Genotyping Extension — (optional) confirm the edit at the molecular level using PCR and agarose gel electrophoresis
Designed for high school and college or university lab classes, the Out of the Blue CRISPR and Genotyping Extension Kits earned the 2020 Science Educators’ Best of Show award for “Best New Technology Innovation for Science” from the National Science Teaching Association (NSTA).
CRISPR Gene Editing Is More Than Just a Cut
CRISPR is hailed as a revolutionary tool used by scientists to rewrite DNA and tackle issues like disease-causing mutations head-on. Yet, while the CRISPR-Cas9 system often takes the spotlight for its precise DNA cutting, it's just the opening act. The cell's DNA repair machinery must also step in to turn those cuts into desired and transformative genetic edits.
Where other educational kits merely showcase the cutting function of CRISPR-Cas9, the Bio-Rad Out of the Blue Gene Editing Kit demonstrates the need for both cutting and repair in gene editing. Students utilize CRISPR-Cas9 alongside homology-directed repair (HDR) to both cut and insert a stop codon into the chromosomal lacZ gene of E. coli. They can then use PCR to confirm that an edit was made.
The carefully designed bacterial strain and plasmids in the Out of the Blue Gene Editing Kit allow students to safely explore and learn about the components of CRISPR gene editing, all with minimal equipment required. Clearly visible results allow students to see what happens if the Cas9 enzyme does not have a guide RNA to direct a cut, and students also witness the vital role of DNA repair machinery in the gene editing process.
In the time it takes to perform a bacterial heat shock transformation, students perform true CRISPR-Cas9 genome editing to change a gene in a living cell, as is done in real-world applications of the technology!
Features and Benefits
- Hands-on CRISPR gene editing — students get direct experience with this powerful technology
- Safety — familiar and common reagents and non-pathogenic, non-biofilm-forming bacteria generate a phenotype that is common to molecular biology; instructions for safe disposal are also included
- Accessibility — does not require specialized equipment or techniques
- Visible phenotype — results can be observed with the naked eye in as little as 22 hr
- More than just a cut, more than a knockout — use CRISPR-Cas9 technology to cut and introduce a stop codon into the chromosomal lacZ gene of E. coli
- Comprehensive curriculum
- Background information about the components of a CRISPR-Cas9 system
- The science and math behind Cas9 accuracy and flexibility — how is Cas9 different from restriction enzymes?
- Paper model activity — explore how the components of a CRISP-Cas9 gene editing system work together before performing the lab
- Controls — avoid misconceptions by demonstrating the role of each component used in CRISPR-Cas9 gene editing
- Guided bioinformatic analysis of CRISPR targets students design themselves — reinforce understanding of the promise, challenges, and risks of CRISPR gene editing
Activity Overview
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1
Modeling the components of the CRISPR-Cas9 System
Use a paper model to understand the components of the CRISPR-Cas9 system and the math behind its accuracy
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2
lacZ CRISPR Gene Editing Laboratory
Use CRISPR-Cas9 to edit the chromosomal lacZ gene in E. coli
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3
Genotyping Extension Laboratory (Optional)
Use PCR and gel electrophoresis to confirm the edit in transformants
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4
Identification and Bioinformatic Analysis of Cas9 Target Sites in Human Disease
Use bioinformatics to design Cas9 target sites in the human genome and determine risk for off-target effect
*Requires purchase of the Out of the Blue Genotyping Extension
CRISPR Teaching Resources
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Webinars and Workshops
New to CRISPR? Join us at an upcoming webinar or workshop, or view recorded webinars.
CRISPR-Cas Gene Editing Teaching Resources
Access background information and links to videos, articles, and other resources to help you teach about CRISPR gene editing in your classroom.
CRISPR PowerPoint Presentation
Use this editable student-facing slide deck to help guide your students through the CRISPR gene editing lab activity.
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CRISPR Poster (Free)
The poster features medical breakthroughs enabled by CRISPR gene editing technology, as well as a timeline of this and other Nobel prizes earned by women in science.
Kit Manual Translations
Translations of the Instructor and Student Guide in Spanish (Español), French (Français), and German (Deutsch) are available.
YouTube CRISPR Gene Editing Playlist
Access technique videos and have your students gain perspectives about CRISPR from some of the industry’s leading experts.
Additional Information
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This hands-on activity is integrated with the Lt Biology Collection, the online learning platform from ADInstruments.
Specifications
Kit Contents
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CRISPR-Cas9 gene editing kit (12012608EDU) and genotyping extension kit (120126078EDU) package with agarose powder, Fast Blast DNA stain, and buffer. For educational use only — not for self-administration.
Refills
CRISPR-Cas9 gene editing kit refill, includes bacteria, plasmids, KIX Mix, spectinomycin, arabinose, and transformation solution. LB nutrient agar powder (1660600EDU x 2) and LB broth capsule (1660412EDU) not included. For educational use only — not for self-administration.
CRISPR-Cas9 genotyping extension refill, includes primers, positive control DNA, master mix, molecular weight ruler, and loading dye. Instagene Matrix (7326030EDU), Out of the Blue CRISPR Kit (12012608EDU), and electrophoresis reagents also required, sold separately. For educational use only.
