Overview
1 x 96-well plate, includes coupled magnetic beads and detection antibodies, buffers, lysis factor QG, diluent, streptavidin-PE, 96-well flat bottom plate, and plate seals for the detection of 8 Akt pathway markers
Safety Data Sheet
Description
Description
Use the Bio-Plex Pro cell signaling Akt 8-plex panel, an all-in-one premixed multiplex kit, for detecting the following phosphorylated analytes from cell lysates derived from cell culture or tissue samples:
- Akt (Ser473)
- BAD (Ser136)
- GSK-3α/β (Ser21/Ser9)
- IRS-1 (Ser636/Ser639)
- mTOR (Ser2248)
- p70 S6 kinase (Thr389)
- PTEN (Ser380)
- S6 ribosomal protein (Ser235/Ser236)
This assay panel measures the relative concentration of the phosphorylated protein.
Benefits
- All-in-one premixed kit
- Exceptional sensitivity (0.15–0.3 µg/well) and broad dynamic range
- Get started right away with the included assay quick guide
- Simplified assay processing with magnetic bead–based workflow to enable automation
Kit Components
- Coupled 6.5 µm magnetic beads
- Detection antibodies
- Cell lysate
- Cell lysis buffer
- Cell lysis factor QG
- Cell wash buffer
- Bead resuspension buffer
- Detection antibody diluent
- Wash buffer
- Streptavidin-PE
- 96-well flat bottom plate
- Sealing tape
Other Available Cell Signaling Assays
Browse our latest list of available phosphoprotein, total target, and housekeeping protein assays.
Other Assay Formats Available
Premixed all-in-one kits or custom Bio-Plex® x-Plex™ assays.
Instrument Compatibility
Compatible with these readers and wash stations:
- Bio-Plex® 200 or Luminex 200 system
- Bio-Plex® 3D or Luminex FLEXMAP 3D system
- Bio-Plex® MAGPIX™ or Luminex MAGPIX system
- Bio-Plex Pro™ wash stations
More Information
To learn more about performance characteristics or how to set up your cell signaling assays for optimal performance and sensitivity, refer to the documents listed below: