Helios® Gene Gun System
The Helios Gene Gun is a convenient handheld device that provides direct, rapid, and versatile gene delivery into a range of targets in vivo.
Overview
The Helios Gene Gun is a convenient handheld device that provides rapid and direct transfection into a range of targets in vivo. The unit uses an adjustable low-pressure helium pulse to sweep DNA-, RNA-, or biomaterial-coated gold microcarriers from the inner wall of a small plastic cartridge directly into target cells.
Key Features
- Provides easy-to-use, rapid, versatile gene delivery independent of target cell type
- Facilitates both transient and stable expression
- Requires only small amounts of DNA and cells; no carrier DNA needed
- Enables codelivery of more than one plasmid
- Allows transfer of large DNA fragments
- Targets intracellular gene delivery to many cells
- Works for both in vitro and in vivo transformation
- Delivers no extraneous genes or proteins
Helios® Gene Gun Training Video.
This video provides an overview of the Helios Gene Gun Biolistic Particle Transfection System, including instrument setup, microcarrier and cartridge preparation, and instrument operation.
Specifications
Helios Gene Gun System
Functional
Maximum current
10 mA peak
Input voltage
9 V alkaline battery, replaceable
Battery life
1,000 discharges in continuous use
Gas pressure
600 psi maximum helium
Safety relief pressure
700 ± 35 psi at regulator assembly
Regulator adjustment
800 psi limit maximum
Discharges
12 per cylinder, mechanical indexing
Environmental
Operating temperature
10–32°C (50–90°F); 30–80% humidity
Storage temperature
0–60°C (32–110°F); 10–90% humidity
Physical
Dimensions (W x D x H)
20 x 25 cm (8 x 10"; approximate)
Weight
1.43 kg (3.15 lb)
Use
For Research Use Only. Not for use in diagnostic procedures.
Tubing Prep Station
Electrical
Maximum current
62 mA/125 mA peak
Input voltage
220/240 V or 100/120 V
Functional
Input frequency
50/60 Hz
Safety relief pressure
30 ± 1.5 psi at regulator assembly
Speed
30 rpm nominal
Tubing fill
Manual
Physical
Construction
Aluminium and acrylic
Dimensions (W x D x H)
85 x 10 cm (33.5 x 4"; approximate)
Weight
5.08 kg (11.2 lb)
Ordering
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(Discontinued) Helios Gene Gun Kit
1652411
100/120 V, handheld biolistic system, includes gene gun, 5 O-rings, 5 barrel liners, 5 white cartridge holders, cartridge extractor tool, 9 V battery.
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(Discontinued) Helios Gene Gun System
1652431
100/120 V, handheld biolistic system, includes helium hose assembly with regulator, tubing prep station (#1652418), syringe kit, Tefzel tubing, tubing cutter, optimization kit (#1652424)
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(Discontinued) Tubing Prep Station
1652420
220/240 V, Helios gene gun sample preparation station, for the preparation of microparticle cartridges
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(Discontinued) Voltage Converter
1652259
Pkg of 1, voltage converter, for use with PDS-1000/He system on 220–240 V lines
Accessories
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(Discontinued) Helium Hose Assembly
1652412
Pkg of 1, Helios gene gun accessory, connects to helium source for safety and consistent helium delivery, with Swagelok quick-connect fittings
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(Discontinued) Helium Regulator
1652413
Pkg of 1, pressure regulator for use with Helios gene gun, CGA 580 female fitting (U.S. standard) with pressure relief valve, max. pressure 2,600 psi
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(Discontinued) Low-Pressure Helium Regulator for Helios Gene Gun
1652414
Pkg of 1, pressure regulator for use with Helios gene gun, CGA 580 female fitting (U.S. standard) with pressure relief valve, max. pressure 400 psi
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(Discontinued) Tubing Prep Station
1652418
100/120 V, Helios gene gun sample preparation station, for the preparation of microparticle cartridges
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(Discontinued) Tubing Prep Station
1652420
220/240 V, Helios gene gun sample preparation station, for the preparation of microparticle cartridges
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(Discontinued) Syringe Kit
1652421
Kit for loading gold suspension into Tefzel tubing, includes syringe adaptor tubing, 5 x 10 ml syringes, syringe adaptor fitting, 5 Luer-Lok fittings
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(Discontinued) Tubing Cutter
1652422
Pkg of 1, instrument for cutting Tefzel tubing, for use with the Helios gene gun, includes 10 razor blades
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(Discontinued) Helios Gene Gun Optimization Kit
1652424
Optimization kit for the Helios gene gun, includes 0.6 μm, 1.0 μm and 1.6 μm gold microcarriers, PVP, desiccant, 5 cartridge storage vials, 50 feet of Tefzel tubing, for 1,000 samples
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(Discontinued) Cartridge Kit
1652440
Kit for preparing Helios gene gun cartridges, includes 0.5 g PVP, 5 desiccant pellets, 5 cartridge collection/storage vials, 15 m of Tefzel tubing
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(Discontinued) 0.6 µm Gold Microcarriers
1652262
0.25 g, 0.6 μm diameter, gold microcarriers for use with the PDS-1000/He and Helios gene gun systems
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(Discontinued) 1.0 µm Gold Microcarriers
1652263
0.25 g, 1.0 μm diameter, gold microcarriers for use with the PDS-1000/He and Helios gene gun systems
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(Discontinued) 1.6 µm Gold Microcarriers
1652264
0.25 g, 1.6 μm diameter, gold microcarriers for use with the PDS-1000/He and Helios gene gun systems
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(Discontinued) Nitrogen Regulator for US Standard Connections
1652425
Pkg of 1, nitrogen regulator, for drying Tefzel tubing during Helios gene gun sample preparation
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(Discontinued) O-Rings
1652416
Pkg of 5, O-rings for use with the Helios gene gun system (#1652431, #1652432)
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(Discontinued) Barrel Liner
1652417
Pkg of 5, sample preparation accessory for Helios gene gun
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(Discontinued) Cartridge Holder
1652426
Pkg of 5, accessory for loading microparticle-coated cartridges into the Helios gene gun, white
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(Discontinued) Cartridge Extractor Tool
1652435
Pkg of 1, tool for removing discharged cartridges, for use with the Helios gene gun (#1652431, #1652432)
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(Discontinued) Battery
1652436
Pkg of 1, 9 V alkaline battery, for use with the Helios gene gun, provides 1,000 discharges in continuous use
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(Discontinued) Tefzel Tubing
1652441
15 m, tubing for coating with DNA- or RNA-gold microcarrier complexes, used with the Helios® gene gun, .125" (3.175 mm) OD, .093" (2.36 mm) ID
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(Discontinued) Helios Diffusion Screens
1652475
Pkg of 5, screens for use with the Helios gene gun (#1652431, #1652432)
Microcarriers
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(Discontinued) 0.6 µm Gold Microcarriers
1652262
0.25 g, 0.6 μm diameter, gold microcarriers for use with the PDS-1000/He and Helios gene gun systems
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(Discontinued) 1.0 µm Gold Microcarriers
1652263
0.25 g, 1.0 μm diameter, gold microcarriers for use with the PDS-1000/He and Helios gene gun systems
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(Discontinued) 1.6 µm Gold Microcarriers
1652264
0.25 g, 1.6 μm diameter, gold microcarriers for use with the PDS-1000/He and Helios gene gun systems
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