PCR Assays Capture Gene Expression Insights

The introduction of real-time quantitative PCR (qPCR) in the early 1990s opened the door for quantitative gene expression analysis. Still, qPCR depends on standard curves, allowing only relative quantification, and can be susceptible to variations in amplification efficiency. Digital PCR has emerged as a solution that provides highly sensitive absolute quantification of nucleic acids without the need for standard curves.

This ebook explores gene expression testing innovations such as qPCR, dPCR, and multiplexing, including the following:

• A timeline of gene expression assay development
• Tips and tricks for addressing PCR pain points
• Guidelines for gene expression data analysis and experimental reproducibility
• Droplet Digital™ PCR (ddPCR™) workflows for gene expression analysis