How to Measure Adeno-Associated Virus (AAV) Vector Genome Titer
Exact viral titer is critical at all stages of cell and gene therapy development, including dosing, optimizing transduction efficiency, and evaluating potency. Droplet Digital™ PCR (ddPCR™) can directly quantify DNA copies with precision without a standard curve. An optimized protocol is now available that includes step-by-step instructions using AAV as a model system.
Get started with this protocol designed to measure adeno-associated virus (AAV) vector genome titer using ddPCR for both in-process and purified samples.
What you will find in this protocol:
- A detailed list of instruments and reagents
- A step-by-step protocol
- How to perform viral titer calculations
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