Bio-Plex Pro™ RBM Apoptosis Panel 2 #171WAR2CK

Use the Bio-Plex Pro RBM apoptosis panel 2 multiplex assay for detecting the intracellular proteins involved in the commitment, onset, and induction of apoptosis by the intrinsic pathway listed below:

• Bad
• Bax/Bcl-2 dimer
• Bcl-xL
• Bim
• Mcl-1

For use with the following sample types:

• Cell lysates
• Tissue lysates

Benefits

• Optimized for high precision and lot-to-lot reproducibility of sample measurements
• Magnetic beads for simplified plate processing
• 2-level quality controls with kit lot-specific ranges
• Assay quick guide to get you started right away

Kit Components

• • 6.5 µm magnetic capture beads
  • Detection antibodies
  • Standards
  • 2-level controls
  • Standard diluent
  • Blocking buffer
  • Lysate dilution buffer (LDB)
• • Cytosolic extraction buffer (CEB)
  • 10x assay buffer
  • 10x streptavidin-PE
  • Flat bottom plate
  • Plate seals
  • Instructions

Other Available Apoptosis Assays

Run all three Bio-Plex Pro RBM apoptosis panels for a more complete picture of the intrinsic pathway:

• Panel 1 (171-WAR1CK) detects Bak, Bax, Lamin B, and Smac
• Panel 2 (171-WAR2CK) detects Bad, Bax/Bcl-2 dimer, Bcl-xL, Bim, and Mcl-1
• Panel 3 (171-WAR3CK) detects active caspase-3, Bcl-xL/Bak dimer, Mcl-1/Bak dimer, and survivin

Instrument Compatibility

Compatible with these readers and wash stations:

• Bio-Plex 200 or Luminex 200 system
• Bio-Plex 3D or Luminex FLEXMAP 3D system
• Bio-Plex^® MAGPIX™ or Luminex MAGPIX system
• Bio-Plex Pro™ wash stations

More Information

To learn more about performance characteristics and other cancer-related panels, refer to the documents listed below:

• Product information sheet
• Instruction manual
• Quick guide
• Cancer panels product information sheet
• Cell signaling product information sheet