Overview
The Vericheck QX700 ddPCR Empty-Full Capsid Kit provides a fast, precise solution for measuring AAV empty-to-full capsid ratio, capsid titer, and genome titer from crude lysate or purified samples. Because empty-to-full capsid ratio is a critical quality attribute in AAV vector production, the kit delivers consistent, reproducible results from low-sample input with a streamlined workflow to support AAV characterization across development and manufacturing using Droplet Digital™ PCR (ddPCR) technology.
Features & Benefits
- Broad sample compatibility for analysis of crude lysate and purified AAV samples
- Low-sample input enables results from as few as 10^6 virus particles
- High-precision measurements deliver CVs below 5% to support reliable assessment of capsid content
- Streamlined data analysis reports genome titer, capsid titer, and empty-to-full ratio in a single workflow
- Fast turnaround supports efficient AAV vector characterization across development and manufacturing workflows
Applications & Uses
- Measure AAV empty-to-full capsid ratio, capsid titer, and genome titer in a single workflow
- Support AAV vector characterization across development, process optimization, and manufacturing
- Analyze crude lysate and purified samples with low-sample input
- Generate reproducible results to inform capsid quality assessment and process decisions
Product Contents
17011024: Vericheck ddPCR Empty-Full Capsid AAV2 Kit
| Catalog Number | Description | Qty |
|---|---|---|
| 12025906 | Vericheck QX700 ddPCR Empty-Full Capsid Reagent Box | 1 |
| 16012910 | 20x ddPCR ITR2 Assay | 1 |
| 16012911 | 20x ddPCR Capsid Detection Assay | 1 |
| 12022016 | Ligase | 1 |
| 12022074 | 10x Ligase Buffer | 1 |
| 12022017 | Dilution Buffer | 1 |
| 12022018 | Stop Solution | 1 |
| 16012872 | 10x PLA Splint | 1 |
| 10042051 | Dnase | 1 |
| 12022020 | 10x Dnase Buffer | 1 |
| 12007991 | QX700 ddPCR RDQ Supermix | 3 |
| 12022087 | Vericheck QX700 ddPCR Empty-Full Capsid AAV2 Kit Box | 1 |
| 12022076 | AAV2 Antibody 1 | 1 |
| 12022019 | AAV2 Antibody 2 | 1 |
| 12022376 | AAV2 Positive Control | 1 |
Equipment
This kit requires the use of one of the following instruments:
Specifications
Storage at –20°C
Refer to expiration date
Documents
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