JAK2 V617 V617F ddPCR Assay 200R #12026921
Safety Data Sheet
Overview
The JAK2 V617 V617F ddPCR Assay 200R is a high-sensitivity, research-use-only, 10x multiplex assay designed to detect and quantify the V617F mutation in the JAK2 gene in research samples using the naica® and Nio digital PCR platforms and RDG16 cartridges. This assay enables precise and reproducible variant analysis, supporting targeted mutation studies in research applications.
JAK2 encodes a non-receptor tyrosine kinase involved in cytokine and growth factor signaling pathways that regulate cell proliferation and differentiation. Variants in this gene are widely studied for their role in signaling pathway activation and cellular regulation.
This assay provides a robust tool for investigating mutation-specific effects, pathway dynamics, and genetic variation across experimental models. W ith dual-channel detection (Blue/Green), the assay supports sensitive multiplex analysis within a streamlined workflow.
It is fully compatible with naica and Nio® Systems, ensuring flexibility across a range of digital PCR setups. Supplied in a 200-reaction format, this assay is well suited for routine and targeted research workflows.
For optimal performance, it is used in combination with Crystal Universal Reporter 3 and naica ddPCR Mix 10X, delivering reliable, precise, and reproducible digital PCR results.
| Product name | Reference | Assay Information Sheet | Scanning Templates Prism 3 | Scanning Templates Prism 6 | Nio Protocol | Nio Assay |
|---|---|---|---|---|---|---|
| JAK2 (V617, V617F) Crystal Digital PCR® Assay | R51048 | AIS_R51048_JAK2.v2.pdf | ScanningTemplate_Prism3_JAK2_R51048_v2.ncx | ScanningTemplate_Prism6_JAK2_R51048_v2.ncx | NioProtocol_3C-60X-60°C-30s+58°C300s_v3.nioprotocol | NioAssay_3C_JAK2_R51048_v2.nioassay |
Specifications
Documents
Recommended Products
The RDG16 Cartridges are designed for partitioning nucleic acid samples and performing digital PCR with the QX700™ E, QX700™ S, and QX700™ HT Droplet Digital™ PCR Systems.*
*For Research Use Only. Not for use in diagnostic procedures.
The naica® 10X ddPCR Mix is a ready-to-use, two-component solution for high-resolution genetic analysis for use on digital PCR systems such as the QX700™ E, QX700™ S, and QX700™ HT Droplet Digital™ PCR (ddPCR™) Systems.*
*For Research Use Only. Not for use in diagnostic procedures.
Crystal Universal Reporters generate fluorescent signals when activated during a digital PCR reaction using target-specific Crystal Flex Probe detection chemistry, allowing for specific detection and quantification of multiple targets within a single reaction. Ready-to-use 40x reporter solutions (Tube A, 200 reactions) enable detection across multiple fluorescence channels, including blue, green, and red channels on naica® Prism3, Prism6, and Nio® Systems.
Crystal Universal Reporters generate fluorescent signals when activated during a digital PCR reaction using target-specific Crystal Flex Probe detection chemistry, allowing for specific detection and quantification of multiple targets within a single reaction. Ready-to-use 40x reporter solutions (Tube A, 1,000 reactions) enable detection across multiple fluorescence channels, including blue, green, and red channels on naica® Prism3, Prism6, and Nio® Systems.
Crystal Universal Reporters generate fluorescent signals when activated during a digital PCR reaction using target-specific Crystal Flex Probe detection chemistry, allowing for specific detection and quantification of multiple targets within a single reaction. Ready-to-use 40x reporter solutions (Tube A and Tube B, 200 reactions) enable detection across multiple fluorescence channels, including blue, green, red, teal, yellow, and infrared channels on naica® Prism3, Prism6, and Nio® Systems.
Crystal Universal Reporters generate fluorescent signals when activated during a digital PCR reaction using target-specific Crystal Flex Probe detection chemistry, allowing for specific detection and quantification of multiple targets within a single reaction. Ready-to-use 40x reporter solutions (Tube A and Tube B, 1,000 reactions) enable detection across multiple fluorescence channels, including blue, green, red, teal, yellow, and infrared channels on naica® Prism3, Prism6, and Nio® Systems.