Overview
A curated selection of Crystal Digital PCR Assays, sourced directly from Stilla Technologies, is available below. These assays are compatible exclusively with the naica and Nio Systems from Stilla Technologies, and must be run with Crystal Universal Reporters (catalog #12026172, #12026157, #12026173, and #12026136).
How It Works
Crystal Digital PCR Assays are developed with Crystal Flex Probes and are run with Crystal Universal Reporters, forming a two-component detection system:
- Crystal Flex Probes are nonfluorescent oligonucleotides specific to the target. They contain a non-target-specific sequence (a tag oligo) which, when released during the PCR reaction, triggers the fluorescence of its complementary Crystal Universal Reporter
- Crystal Universal Reporters are non-target-specific oligonucleotides that remain silent until activated. They emit a fluorescent signal only when triggered by their specific tag oligo during the PCR reaction
This modular design allows a single set of Crystal Universal Reporters to detect any target, keeping probe costs low while maintaining a consistent, reliable reporter system across all your assays.
Target of Interest Is Present
- Target specificity is achieved with non fluorescent Crystal Probes
- The tag oligos released at each PCR cycle trigger fluorescence of their specific Crystal Universal Reporters
FAQs
Specifications
Ordering
Related Products
Crystal Universal Reporters generate fluorescent signals when activated during a digital PCR reaction using target-specific Crystal Flex Probe detection chemistry, allowing for specific detection and quantification of multiple targets within a single reaction.
Crystal Universal Reporters generate fluorescent signals when activated during a digital PCR reaction using target-specific Crystal Flex Probe detection chemistry, allowing for specific detection and quantification of multiple targets within a single reaction.
Crystal Universal Reporters generate fluorescent signals when activated during a digital PCR reaction using target-specific Crystal Flex Probe detection chemistry, allowing for specific detection and quantification of multiple targets within a single reaction.
Crystal Universal Reporters generate fluorescent signals when activated during a digital PCR reaction using target-specific Crystal Flex Probe detection chemistry, allowing for specific detection and quantification of multiple targets within a single reaction.
The naica® 10X ddPCR Mix is a ready-to-use, two-component solution for high-resolution genetic analysis for use on digital PCR systems such as the QX700™ E, QX700™ S, and QX700™ HT Droplet Digital™ PCR (ddPCR™) Systems.*
The RDG16 Cartridges are designed for partitioning nucleic acid samples and performing digital PCR with the QX700™ E, QX700™ S, and QX700™ HT Droplet Digital™ PCR Systems.*
*For Research Use Only. Not for use in diagnostic procedures.