UNO® Monolith Cation Exchange Columns

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Overview

UNO monolith ion exchange columns contain a patented continuous-bed matrix, allowing biomolecule separations at high flow rates. The UNO S columns are for strong cation exchange.
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Description

Description

UNO monolith ion exchange columns contain a patented* continuous-bed matrix, allowing biomolecule separations at high flow rates without sacrificing resolution or capacity. In continuous-bed technology, advanced monomers and ionomers are polymerized directly in the column and aggregate into a dense homogeneous network of nodules. The resulting rigid matrix contains interconnecting channels large enough to allow fast hydrodynamic flow at low backpressures. The continuous-bed matrix yields a column with exceptional resolution, speed, capacity, and value for the separation of biomolecules. The homogeneous, nonporous continuous-bed matrix allows extremely fast mass transfer of proteins and eluent to the ionic functional groups.

The UNO S columns are for strong cation exchange. Choose from the following UNO columns:

  • UNO glass columns — provide high-resolution separation of biomolecules. The transparent glass tube allows easy bed inspection and column troubleshooting. Three column sizes provide flexibility for purification protocols
  • UNO replacement columns — provide a simple bed replacement as an alternative to purchasing a new column; two column sizes are available

* U.S. patent 6,423,666.

Ordering

Ordering

items

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(Discontinued) UNO S1 Column
7200021
Pkg of 1, 7 x 35 mm, 1.3 ml, prepacked monolith ion exchange column, 20 mg max. protein load, 0.5–5.0 ml/min flow rate, operating pressure 700 psi
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(Discontinued) UNO S6 Column
7200023
Pkg of 1, 12 x 53 mm, 6 ml, prepacked monolith ion exchange column, 90 mg max protein load, 0.5–8.0 ml/min flow rate, operating pressure 700 psi
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(Discontinued) UNO S12 Column
7200025
Pkg of 1, 15 x 68 mm, 12 ml, prepacked monolith ion exchange column, 180 mg max. protein load, 0.5–8.0 ml/min flow rate, operating pressure 700 psi
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(Discontinued) UNO S1R Column
7200031
Pkg of 1, 7 x 35 mm, 1.3 ml, prepacked replacement column
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7200029
Pkg of 1, 4.6 x 10 mm, 0.16 ml, prepacked polishing column for late-stage purification

Accessories

Accessories

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(Discontinued) UNO S6R Column
7200033
Pkg of 1, 12 x 53 mm, 6 ml, prepacked replacement column, 90 mg max. protein load, 0.5–8.0 ml/min flow rate, operating pressure 700 psi
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(Discontinued) UNO S12R Column
7200035
Pkg of 1, 15 x 68 mm, 12 ml, prepacked replacement column, 180 mg max. protein load, 0.5–8.0 ml/min flow rate, operating pressure 700 psi
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7500554
Pkg of 10, post-pump fittings, includes Delrin nut, ferrules, lock ring, for use with BioLogic DuoFlow systems
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7500568
Fittings kit, includes 2 nuts and 4 ferrules to connect UNO Column to an HPLC system, for use with BioLogic DuoFlow™ and NGC™ Systems
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(Discontinued) UNO M6 Fittings Kit
7500567
Fittings kit, includes 2 nuts and 4 ferrules to connect UNO column to an FPLC system, for use with BioLogic DuoFlow™ and NGC™ systems

Documents

Documents

Number Description Options
6498
Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C
[ Add to Cart (Free) ]
2303
Purification of Insulin on a UNO® S1 Column, Rev A
2283
Purification of the Components of Human Macular Carotenoid-Binding Complex, Rev A
4006085
Instruction Manual, UNO Column Bed Removal Tool, Rev A
4006084
Instruction Manual, UNO Q&S Continuous Bed Ion Exchange Replacement Column, Rev A
4006078
Instruction Manual, UNO® Q&S Polishing Column for Continuous Bed Ion Exchange, Rev A
4006076
Instruction Manual, UNO Q and S Continuous Bed Ion Exchange Columns