SEQuoia Express Stranded RNA Library Prep Kit

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SEQuoia Express

Overview

SEQuoia Express Stranded RNA Library Prep Kit enables customers to overcome the challenge of a laborious library prep workflow by providing a cost-effective 3-hour, 3-tube, automation-friendly solution for constructing robust libraries that efficiently capture long RNA transcripts (>200bp). 

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Description

Description

Frequently Asked Questions

SEQuoia FAQ

Learn how to use the SEQuoia Express Kit

View SEQuoia Express FAQ

SEQuoia Express Stranded RNA Library Prep Kit is a fast, high-performance RNA-Seq library preparation kit used to construct a sequence-ready library comprised of messenger RNA and long-noncoding RNA biotypes that is compatible with Illumina platforms from a broad range of total RNA inputs. Featuring SEQzyme, a proprietary engineered enzyme that couples cDNA synthesis with adapter addition in a continuous synthesis reaction, this kit streamlines library construction by reducing the number of components to just three tubes, significantly minimizing the number of pipetting steps and shortening the protocol time to under three hours. 

SEQuoia Express Stranded RNA Library Prep Kit is compatible with SEQuoia RiboDepletion Kit for removal of constructs originating from ribosomal RNA and SEQuoia Dual-Indexed Primers, enabling a user to pool libraries and run them on a single flow-cell, greatly reducing the cost of over sequencing. Furthermore, it comes with a pre-packaged bioinformatics pipeline with the options to select a researcher’s local infrastructure or the cloud-based web application. This rapid and cost-effective workflow provides a seamless end-to-end solution for researchers who want to generate the highest quality data with minimal chance of error.

 

Features and Benefits

  • Simplified workflow that can be completed under 3 hours
  • Convenient 3-tube configuration minimizes pipetting steps and hands-on time
  • Efficient and unbiased capture of coding and non-coding long RNA
  • Validated with a broad range of RNA input
  • Integrated with bioinformatics solution

Applications and Uses

For researchers and drug developers who require high-quality, complex, and unbiased sequencing libraries to profile differential expression patterns of mRNA and long non-coding RNA biotypes and be able to quickly assess the quality of their sequencing data. Main applications include:

  • Liquid biopsy
  • Single nucleotide variation discovery
  • Gene fusion identification
  • Splice junction identification

Included Components

Each kit comes with three reagent components: reaction mix, enzyme mix, and an amplification mix. The kit should be stored at -20°C.

 

SEQuoia Express Stranded RNA Library Prep Technology FAQs

SEQuoia Express Stranded RNA Library Prep Technology

How does SEQuoia Express retain strand information?

Why is SEQuoia Express more efficient in capturing long RNAs?

How does SEQuoia Express construct library with a shorter workflow?

What is UMI and what is the purpose of having UMI in our adapter design?

What does a typical Bioanalyzer trace of a SEQuoia Express library look like?

SEQuoia Express Stranded RNA Library Prep Kit Information

What is included in the kit?

What is the input range for the kit?

What is the shelf life of the kit?

Do you offer sample indexes work with the kit?

Do you offer a ribosomal RNA depletion product to work with this library prep kit?

SEQuoia Express Stranded RNA Library Prep Data Analysis

Do you offer a data analysis pipeline to work with SEQuoia Express Stranded RNA Library Prep Kit?

What is the difference between the web hosted solution and the toolkit?

Do I require to use SeqSense analysis pipeline to analyze my sequencing data?

Configuration Options

Catalogue Number Description Size
12017297 SEQuoia Express Stranded RNA Library Prep Kit 24 rxn
12017265 SEQuoia Express Stranded RNA Library Prep Kit 96 rxn

Related Products

Related Categories

Specifications

Specifications

Capture Range
mRNA and long non-coding RNA > 200 bp
Input Range
1 ng – 1 ug total RNA
Workflow Time
3 hours
Hands-on Time
45 min
Kit Component
3 tubes
Strand Specificity
≥ 98% Stranded
Shelf Life
Guaranteed for 12 months from shipment when properly stored at -20°C

Ordering

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items

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12017297
12017297

Pkg of 1, kit for 24 reactions, includes 600 µl SEQuoia Express Reaction Mix, 120 µl SEQuoia Express Enzyme Mix, and 600 µl SEQuoia Express Amplification Mix

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12017265
12017265

Pkg of 1, kit for 96 reactions, includes 2 x 1,200 µl SEQuoia Express Reaction Mix, 480 µl SEQuoia Express Enzyme Mix, and 2 x 1,200 µl SEQuoia Express Amplification Mix

Accessories

Accessories

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tbc-0802_tn.jpg
TBC0802
Pkg of 20, bag of 12 x 8 thin-wall polypropylene tube strips and 12 x 8-cap strips for PCR, natural (1,920 tubes and 1,920 caps)
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tbc-1202_tn.jpg
TBC1202
Pkg of 20, bag of 8 x 12 thin-wall polypropylene tube strips and 8 x 12-cap strips for PCR, natural (1,920 tubes and 1,920 caps)

Documents

Documents

Number Description Options
3330
SEQuoia Express Stranded RNA Library Prep Kit, Product Information Sheet
10000151027
Instruction Manual, SEQuoia Express Stranded RNA Library Prep Kit