Protein Expression and Purification Series

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Protein Expression and Purification Series

Overview

Bring the world of cancer research and biomanufacturing to your students with this modular 5–7 period series of laboratory activities during which students express, purify, and evaluate the enzymatic activity of DHFR.

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Description

Description

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The Protein Expression and Purification Series provides students with the opportunity to express, affinity purify, and evaluate enzymatic activity of DHFR. DHFR is an essential protein in nucleic acid synthesis and was one of the first targets for cancer chemotherapy. The scalability of this particular affinity purification process provides an adaptable set of techniques and content to match the goals of the beginning protein educator up to an advanced college level course in biomanufacturing. The series provides a fully developed and ready-to-go lab course, including relevant background, protocols that work, and student assessment.

Features and Benefits

Designed by undergraduate and community college educators, this course was specifically developed to ensure success for you and your students. The modular nature of the series allows you to choose from three different purification options depending on your teaching goals and equipment resources:

  • Centrifugation Purification Process – allowing protein purification using a 16K Microcentrifuge
  • Hand-packed Purification Process – for use with chromatographic purification instrumentation and allowing students to pour their own columns
  • Prepacked Purification Process – providing the best experience with chromatographic purification instrumentation through the quality of prepacked cartridges

An Assessment Module is also available to complement the hands-on laboratory activities that the series provides. This unique assessment guide provides ideas for using formative assessment in your class to guide and increase learning while students perform the lab activities. At the end of the lab series use the summative assessment to evaluate the final learning levels of your students. The assessment tool is arranged according to learning levels so that you can choose what questions to modify instruction and evaluate the comprehension of your students.

The Protein Expression and Purification Series allows students to apply molecular biology skills and techniques to complete a real-world research-level project, potentially encouraging them to pursue a career in research.

More Information

  • 3–5 weeks of laboratory activities made up of four modular kits
  • Provides sufficient materials for 12 student workstations for the Centrifugation Purification Process and four student workstations for the Hand-packed and Prepacked Purification Processes, up to four students per workstation
  • For information about column fittings and adapters, visit Chromatography Accessories

Ordering

Ordering

items

Use the filters below to refine results!

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1665040EDU
Biotechnology study kit, includes 4 modules with lab activities from protein expression and purification using spin columns to SDS-PAGE and enzymatic activity analysis, curriculum
protein-expression-and-purification-series-hand-packed-purification-process-1665045-tn.jpg
1665045EDU
Biotechnology study kit, includes 4 modules with lab activities from protein expression and purification using hand-packed columns to SDS-PAGE and enzymatic activity analysis, curriculum
protein-expression-and-purification-series-prepacked-purification-process-1665050-tn.jpg
1665050EDU
Biotechnology study kit, includes 4 modules with lab activities from protein expression and purification using prepacked cartridges to SDS-PAGE and enzymatic activity analysis, curriculum
protein-expression-and-purification-series-formative-and-summative-assessment-module-1665070-tn.jpg
1665070EDU
Assessment guide provides ideas for using formative assessment and summative assessment to aid in evaluating the learning levels of your students

Modules

Modules

1665055EDU
First module in Protein Expression and Purification Series classroom kit, includes BL21(DE)3 containing pDHFR, solutions, culturing reagents, and more
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1665060EDU
SDS-PAGE gel electrophoresis reagents includes MW standards, TGS buffer, Laemmli sample buffer, and Bio-SafeTM Coomassie stain
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1665041EDU
Third module purification option in Protein Expression and Purification Series classroom study kit, includes Profinity™ IMAC Ni-charged resin, Micro Bio-Spin® 6 Tris columns, and more
protein-expression-and-purification-series-hand-packed-purification-process-1665046-tn.jpg
1665046EDU
Third module purification option in Protein Expression and Purification Series classroom study kit, includes Profinity™ IMAC Ni-charged resin, glass Econo-Column® columns, and Micro Bio-Spin® 6 Tris columns
protein-expression-and-purification-series-prepacked-purification-process-1665051-tn.jpg
1665051EDU
Third module purification option in Protein Expression and Purification Series classroom kit; includes EconoFit Profinity IMAC cartridges and Micro Bio-Spin 6 Tris columns
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1665065EDU
Fourth module in Protein Expression and Purification Series classroom study kit, includes dihydrofolic acid substrate and NADPH cofactor

Refills

Refills

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1665057EDU
Consumables refill kit for use with Growth and Expression Module includes BL21(DE)3 containing pDHFR, solutions, and culturing reagents
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(Discontinued) LB Agar Powder
1660600EDU
20 g, makes forty 60 mm agar plates; education use only
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1660472EDU
500 g, makes one thousand
60 mm agar plates; education use only
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(Discontinued) Ampicillin
1660407EDU
30 mg, lyophilized reagent, antibiotic for use with the pGLO™ Bacterial Transformation Kit (#166-0003EDU); education use only
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1662403EDU
100 ml, 10x Phosphate Buffered Saline (PBS); education use only
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(Discontinued) Sterile Water
1632091EDU
500 ml, sample preparation buffer reagent; education use only
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(Discontinued) Petri Dishes
1660470EDU
Pkg of 500, 60 mm x 15 mm, sterile; education use only
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(Discontinued) Inoculation Loops
1660471EDU
Pkg of 100, 10 μl, sterile; education use only
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2239430EDU
Pkg of 500, graduated micro test tube with pierceable top, 2 ml, autoclavable, natural; education use only
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(Discontinued) 1.5 ml Conical Tubes
2240100EDU
Pkg of 500, screwcap micro test tube, unsterilized, with separate O–ring screwcaps; education use only
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(Discontinued) 1.5 ml Conical Tubes
2240110EDU
Pkg of 500, screwcap micro test tube, sterilized, with installed O–ring screwcaps; education use only
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1610732EDU
1 L, 10x premixed protein electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3; education use only
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1610737EDU
30 ml, premixed protein sample buffer for SDS-PAGE; education use only
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1610786EDU
1 L, premixed protein staining solution for polyacrylamide gels; education use only
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1610374EDU
500 µl, mixture of 8 blue-stained recombinant proteins (10–250 kD) with two pink reference bands (25 and 75 kD), 50 applications; education use only
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1660479EDU
Pkg of 8, microcentrifuge tube rack, holds 12 tubes; education use only
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(Discontinued) Gel Staining Trays
1660477EDU
Pkg of 4; education use only
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7371007EDU
Pkg of 2, 1.0 x 5 cm, glass chromatography column, max. vol. 4 ml, cross-sectional area 0.79 cm2, for use in low-pressure chromatography; educational use only
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7326221EDU
Pkg of 25, prepacked spin columns with Bio-Gel® P-6 in Tris buffer, sample volume 10–75 μl, 6,000 MW limit, includes 50 collection tubes; education use only
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(Discontinued) Flow adaptor
7380014EDU
Pkg of 1, 1.0 cm column ID,
1–7 cm functional length, for use with Econo-Column® chromatography columns; education use only

Documents

Documents

Number Description Options
6673
Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories
6160
Biotechnology Explorer™ Protein Expression and Purification Series Planning Guide, Rev A
1665067
Instruction Manual, Protein Expression and Purification Series, Biotechnology Explorer™, Rev A
6933
Fast Electrophoresis and Western Blotting Flier