Profinity epoxide resin is an activated affinity chromatography support for the immobilization of biomolecules. Profinity epoxide is a useful support for the immobilization of ligands that contain nucleophiles, such as amino, thiol, or hydroxyl groups. These groups couple to the epoxy groups on the support, which is then used for the purification of proteins, carbohydrates, or DNA.
Profinity epoxide resin is based on UNOsphere beads, which have structural characteristics that result in superb physical strength, excellent flow properties, low backpressure, and an open pore structure. The open pore structure allows coupling of large ligands for the purification of large targets. Examples include protein A for purification of monoclonal antibodies, and other ligands for purification of recombinant proteins, such as those containing maltose-binding protein and calmodulin. In addition, Profinity epoxide allows operation at very high flow rates, resulting in increased throughput and rapid column cleaning between chromatography runs. It is supplied as a dry powder (1 g of powder gives ~8 ml final volume).
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