Profinia Protein Purification System Buffers and Reagents

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Overview

Individually packaged reagents are available for use with the Profinia system. Reagents can also be used for general affinity purification workflows, from sample preparation to protein detection.
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Description

Description

Individually packaged reagents are also available for use with the Profinia system. While designed specifically to work with the Profinia system, these reagents also have general applicability within all stages of a purification workflow, from sample preparation to protein detection.

Profinia Bottled Buffers
For added convenience, preformulated buffers are available for native IMAC, GST, and desalting purifications.

Profinia Control Lysate
The Profinia control lysate is a lyophilized, dual-tagged 51 kD target protein that is meant to eliminate concerns over variability in purification buffer solutions or in the purification matrix itself. The control lysate facilitates system setup and initial purification and is included in every Profinia native IMAC and GST starter kit.

Urea and Glutathione Reagents
Powdered urea and glutathione reagents are available for use with denaturing IMAC and GST applications, respectively. The urea packs are ideal for solubilizing and denaturing proteins in E. coli extracts. The glutathione pack is essential for eluting GST fusion proteins from immobilized glutathione resins.

His and GST Antibodies
Histidine (His)-tagged and GST-tagged monoclonal antibodies are used to detect target protein expression of overexpressed 6x His and GST fusion proteins. They are supplied at a concentration of 1 mg/ml in phosphate buffered saline, pH 7.4 with 0.05% NaN3.

Ordering

Ordering

items

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(Discontinued) Glutathione Pack
6200202
1.23 g, powdered reagent, for denaturing GST applications on the Profinia protein purification system
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(Discontinued) His Antibody
6200203
Pkg of 1 vial, 1 mg/ml, monoclonal antibody against histidine (His)-tagged proteins, for use with the Profinia protein purification system
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(Discontinued) GST Antibody
6200204
Pkg of 1 vial, 1 mg/ml, monoclonal antibody against GST-tagged proteins, for use with the Profinia protein purification system
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6200205
125 ml, preformulated concentrate, 600 mM KCl, 100 mM KH2PO4, 10 mM imidazole, pH 8.0, for use with His-tag protein purification
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6200206
125 ml, preformulated concentrate, 600 mM KCl, 100 mM KH2PO4, 10 mM imidazole, pH 8.0, for use with His-tag protein purification
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6200207
100 ml, preformulated concentrate, 600 mM KCl, 100 mM KH2PO4, 20 mM imidazole, pH 8.0, for use with His-tag protein purification
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6200208
100 ml, preformulated concentrate, 600 mM KCl, 100 mM KH2PO4, 500 mM imidazole, pH 8.0, for use with His-tag protein purification
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(Discontinued) GST Lysis buffer, 2x
6200213
125 ml, preformulated concentrate, 300 mM NaCl, 20 mM Na2HPO4, 10 mM EDTA, pH 7.4, for use with GST-tag protein purification
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(Discontinued) GST Wash buffer, 2x
6200214
200 ml, preformulated concentrate, 300 mM NaCl, 20 mM Na2HP04, 10 mM EDTA, pH 7.4, for use with GST-tag protein purification
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6200215
Pkg of 1, 2x buffer (pH 8.0) containing Tris (200 mM) and EDTA (10 mM) for use with Profina Protein Purification System (6201016). Must add 1.23 g glutathione (6200202, sold separately) before use.
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(Discontinued) Desalting Buffer, 5x
6200216
200 ml, preformulated concentrate, contains NaCl, KCl, Na2HPO4, KH2PO4, pH 7.0, for use with protein desalting
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6200217
125 ml, preformulated concentrate, 1,000 mM NaCl, 100 mM Tris, pH 8.0, for use with cartridge cleaning
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6200218
125 ml, preformulated concentrate, 2,000 mM NaCl, 400 mM NaOAc, pH 4.5, for use with the cartridge cleaning
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(Discontinued) Storage solution, 2x
6200219
200 ml, preformulated concentrate, 4% C6H5CH2OH (benzyl alcohol), for use with cartridge storage
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6200233
Lyophilized, dual-tagged 51 kD protein for system setup and initial purification, for use with the Profinia protein purification system

Cartridges

Cartridges

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7324600
Pkg of 5, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324602
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324610
Pkg of 5, 1 ml, prepacked Ni-charged column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324612
Pkg of 1, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324614
Pkg of 5, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324620
Pkg of 5, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324622
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324624
Pkg of 5, 5 ml, prepacked affinity columns, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324646
Pkg of 2, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems
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7324647
Pkg of 4, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems
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7324648
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems
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7325304
Pkg of 5, 10 ml, prepacked gel filtration column, 80 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™, Profinia™ and other chromatography systems
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7325312
Pkg of 1, 50 ml, prepacked gel filtration column, 80 mm x 28.2 mm, max. pressure 40 psi, for use with BioLogic™, Profinia™ and other chromatography systems
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7325314
Pkg of 5, 50 ml, prepacked gel filtration column, 80 mm x 28.2 mm, max. pressure 40 psi, for use with BioLogic™, Profinia™ and other chromatography systems

Documents

Documents

Number Description Options
10006042
Instruction Manual, Profinia™ His Antibody, Rev A
10006040
Instruction Manual, Profinia GST Glutathione Pack, Rev B
10006043
Instruction Manual, Profinia™ GST Antibody, Rev A
10006041
Instruction Manual, Profinia Denaturing IMAC Urea Pack, Rev A
10006059
Instruction Manual, Profinia™ Control Lysate, Rev C
10006060
Instruction Manual, Profinia Bottled Buffers, Rev A