Description
Description
PCR Kleen columns are prepacked spin columns for purifying PCR products and other DNA molecules >200 bp directly from reaction mixtures. A simple 4 minute spin effectively removes salts, nucleotides, enzymes, primers, and primer-dimers. Purified DNA fragments are eluted into a collection tube and are immediately available for secondary PCR, subcloning, restriction digests, ligations, sequencing, and other enzymatic manipulations.
Related Technologies
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Specifications
Specifications
Method
Size exclusion chromatography in spin column
Applications
Removal of nucleic acids <32 bases and other reaction mixture components from PCR products >200 bp
Capacity
25–100 µl
Equllibration buffer
10 mm Tris, 1mM EDTA, pH 7.4
Sample preparation time
<4 min
Yield
50–80% recovery
Ordering
Ordering
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(Discontinued) PCR Kleen™ Spin Columns
7326300
Pkg of 25, prepacked spin column, for purification of PCR products and other DNA fragments >200 bp directly from reaction mixtures
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