PCR Kleen™ Spin Columns
PCR Kleen columns are prepacked spin columns for purifying PCR products and other DNA molecules of >200 bp directly from reaction mixtures.
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Overview
PCR Kleen spin columns are prepacked spin columns for purifying PCR products and other DNA molecules of >200 bp directly from reaction mixtures. A simple 4 min spin effectively removes salts, nucleotides, enzymes, primers, and primer-dimers. Purified DNA fragments are eluted into a collection tube and are immediately available for secondary PCR, subcloning, restriction digests, ligations, sequencing, and other enzymatic manipulations.
Specifications
Method
Size exclusion chromatography in spin column
Applications
Removal of nucleic acids <32 bases and other reaction mixture components from PCR products >200bp
Capacity
25–100 µl
Equllibration buffer
10 mm Tris, 1mM EDTA, pH 7.4
Sample preparation time
<4 min
Yield
50–80% recovery
Ordering
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(Discontinued) PCR Kleen™ Spin Columns
7326300
Pkg of 25, prepacked spin column, for purification of PCR products and other DNA fragments >200 bp directly from reaction mixtures
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