Description
Description
PCR Kleen spin columns are prepacked spin columns for purifying PCR products and other DNA molecules of >200 bp directly from reaction mixtures. A simple 4 min spin effectively removes salts, nucleotides, enzymes, primers, and primer-dimers. Purified DNA fragments are eluted into a collection tube and are immediately available for secondary PCR, subcloning, restriction digests, ligations, sequencing, and other enzymatic manipulations.
Specifications
Specifications
Method
Size exclusion chromatography in spin column
Applications
Removal of nucleic acids <32 bases and other reaction mixture components from PCR products >200bp
Capacity
25–100 µl
Equllibration buffer
10 mm Tris, 1mM EDTA, pH 7.4
Sample preparation time
<4 min
Yield
50–80% recovery
Ordering
Ordering
items
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(Discontinued) PCR Kleen™ Spin Columns
7326300
Pkg of 25, prepacked spin column, for purification of PCR products and other DNA fragments >200 bp directly from reaction mixtures
Documents
Documents
Login Required Number Description Options
2311
PCR Kleen Spin Columns Product Information Sheet, Rev D
4006142
Instruction Manual, Quantum Prep PCR Kleen Spin Columns, Biotechnology Explorer, Rev B
2950
Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A