Model 491 Prep Cell and Mini Prep Cell

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Overview

Fractionation by size is an effective enrichment strategy for studies of protein families and posttranslational modifications, because the sizes of these proteins tend to be similar.
Read full description Ordering Information

Description

Description

Fractionation by size (molecular weight) is an effective enrichment strategy for studies of protein families and posttranslational modifications, because the sizes of these proteins tend to be similar.

The Model 491 Prep Cell and Mini Prep Cell separate biomolecules quickly and efficiently by continuous-elution electrophoresis. Samples are electrophoresed through a cylindrical gel matrix, where they are separated into ring-shaped bands. As individual bands migrate off the bottom of the gel, they are collected in discrete liquid fractions available for assay and characterization.

The Model 491 Prep Cell and Mini Prep Cell:

  • Allow resolution of proteins differing in molecular weight by as little as 2%
  • Separate up to 500 mg of total protein
  • Can be used as a complementary separation strategy to 2-D gels as well as for downstream protein purification

The Model 491 Prep Cel has a sample capacity of 1–500 mg/0.5–15 ml.

The Mini Prep Cell has a sample capacity of 0.5–1,000 µg/50–500 µl.

Accessory equipment required to run the Model 491 and Mini Prep Cells includes a fraction collector and power supply such as the PowerPac HV power supply. The Model 491 Prep Celll also requires a peristaltic pump.

Ordering

Ordering

items

Use the filters below to refine results!

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1702928
Continuous-elution electrophoresis cell, 1–500 mg/0.5–15 ml sample capacity (mass/volume), includes gel casting equipment
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1702908
Continuous-elution electrophoresis cell, 0.5-1.0 mg/50–500 µl sample capacity (mass/volume), includes gel casting equipment
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1702915
Continuous-elution electrophoresis cell, 0.5-1.0 mg/50–500 µl sample capacity (mass/volume), includes reagent starter kit
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(Discontinued) Model 491 Prep Cell
1702926
100/120 V, continuous-elution electrophoresis cell, 1–500 mg/0.5–15 ml sample capacity (mass/volume) includes pump, starter kit with protein standard
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(Discontinued) Model 491 Prep Cell
1702927
220/240 V, continuous-elution electrophoresis cell, 1–500 mg/0.5–15 ml sample capacity (mass/volume) includes pump, starter kit with protein standard

Note: power cord not included in U.S.; 220 V cord with appropriate plug is provided only for international customers

Accessories

Accessories

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(Discontinued) Elution Frit Kit
1702948
MW cutoff 3,500, includes 5 dialysis membranes, for use with mini prep cell (#170-2915, 170-2908)
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1702947
Pkg of 1, adaptor kit for 0.8 mm tubing, for use with mini prep cell (#170-2915, 170-2908)
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(Discontinued) Thumbscrews
1702940
Pkg of 4, thumbscrew, for use with Model 491 prep cell (#170-2926, 170-2927)
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1702918
Pkg of 1, accessory for use with mini prep cell (#170-2915, 170-2908)
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1702917
Pkg of 1, replacement, for use with mini prep cell (#170-2915, 170-2908)
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1702916
Pkg of 1, for use with mini prep cell (#170-2915, 170-2908)
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1702913
Kit includes syringe and tubing, for use with mini prep cell (#170-2915, 170-2908)
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1702912
Pkg of 1 assembly, includes elution collection tubing, for use with mini prep cell (#170-2915, 170-2908)
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(Discontinued) Elution Frit Kit
1702911
MW cutoff 6,000, includes 5 dialysis membranes, for use with mini prep cell (#170-2915, 170-2908)
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(Discontinued) Gel Tubes
1702909
Pkg of 2, replacement part for mini prep cell (#170-2915, 170-2908)
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1702944
Pkg of 1, gel casting stand, for use with Model 491 prep cell (#170-2926, 170-2927)
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(Discontinued) Upper Buffer Chamber
1702971
Pkg of 1, replacement chamber for the Model 491 prep cell for continuous-elution gel electrophoresis (PAGE)
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(Discontinued) Lower Buffer Chamber
1702970
Pkg of 1, replacement chamber for the Model 491 prep cell for continuous-elution gel electrophoresis (PAGE)
1702969
Pkg of 1, for use with Model 491 prep cell (#170-2926, 170-2927)
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1702941
Pkg of 1, replacement, for use with Model 491 prep cell (#170-2926, 170-2927)
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1702939
Kit includes sample loading guide and syringe with PTFE tubing, for use with Model 491 prep cell (#170-2926, 170-2927)
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(Discontinued) Frit Kit
1702938
Kit includes support frit and elution frit, for use with Model 491 prep cell (#170-2926, 170-2927)
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(Discontinued) Dialysis Membranes
1702937
Pkg of 5, precut dialysis membrane, for use with Model 491 prep cell (#170-2926, 170-2927)
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(Discontinued) O-Ring Kits
1702936
Pkg of 2 kits, for use with Model 491 prep cell (#170-2926, 170-2927)
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1702935
Kit includes stopcock with tubing, 3 elution buffer circulation lines, electrophoresis/cooling buffer circulation lines, connectors
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1702934
Pkg of 1, assembly, includes feedline connectors, for use with Model 491 prep cell (#170-2926, 170-2927)
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1702933
Pkg of 1, 37 mm ID gel tube assembly, for use with Model 491 prep cell (#170-2926, 170-2927)
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1702932
Pkg of 1, 28 mm ID gel tube assembly, for use with Model 491 prep cell (#170-2926, 170-2927)
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1610323
1 ml, prestained mixture of trypsin inhibitor and carbonic anhydrase for use with the prep cell starter kit (#161-5101)
1615101
SDS-PAGE reagents for Model 491 prep cell, includes running buffer, Tris, APS, TEMED, bis-acrylamide, acrylamide
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1702929
100/120 V, for use with Model 491 prep cell (#170-2926)
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1702930
220/240 V, for use with Model 491 prep cell (#170-2927)

Kits & Reagents

Kits & Reagents

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(Discontinued) TEMED
1610800
5 ml, for catalyst for polyacrylamide gels
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1610700
10 g, ammonium persulfate (APS) catalyst
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(Discontinued) Bis Crosslinker
1610201
50 g, bis-acrylamide crosslinker
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1610148
500 ml, 40% acrylamide and bis-acrylamide solution, 37.5:1
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1610125
150 g, 37.5:1 mixture of 99.9% pure acrylamide and bis-acrylamide powder
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1610122
30 g, 37.5:1 mixture of 99.9% pure acrylamide and bis-acrylamide powder
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1610158
500 ml, 30% acrylamide and bis-acrylamide solution, 37.5:1

Documents

Documents

Number Description Options
M1702925
Instruction Manual, Model 491 Prep Cell, Rev D
4006032
Instruction Manual, Mini Prep Cell
LIT466
Instruction Manual, Mini Prep Cell and Model 491 Prep Cell Starter Kit
10044265
Mini Prep Cell Assembly Guide, Rev B
1964
Model 491 Prep Cell and Mini Prep Cell Brochure, Rev C
3161
Model 491 Prep Cell Bio-Rad Literature