Macro-Prep t-Butyl HIC Resin

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Overview

Macro-Prep hydrophobic interaction chromatography (HIC) resins are methacrylate-based, 50 µm beads used for protein, polypeptide, enzyme, and nucleic acid purification.
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Description

Description

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Macro-Prep Hydrophobic Interaction Chromatography (HIC) Resins are methacrylate-based, 50 µm beads used for protein, polypeptide, enzyme, and nucleic acid purification. They are designed specifically for intermediate purification steps that remove host-cell contaminants from partially purified targets. Due to their rigidity and unique surface chemistry, these hydrophobic chromatography resins are particularly suited for HIC operations requiring high throughput and high recovery of target.

Macro-Prep HIC Resin is available in two functional forms:

  • Macro-Prep Methyl HIC Resin — weakly hydrophobic, for purifying compounds that have strong hydrophobic regions
  • Macro-Prep t-Butyl HIC Resin — mildly hydrophobic, for purifying compounds that have few or weakly hydrophobic regions

Both HIC resins are chemically and thermally stable. These media are autoclavable and can withstand treatment in acid, chaotropic agents, or detergents while retaining high protein-binding capacities.

Macro-Prep t-Butyl HIC Resin operates on an interaction mechanism based on hydrophobicity and charge. The t-butyl groups are mildly hydrophobic. Depending on the pH of loading and elution buffers, the carboxyl groups can be exploited to ionically repel target molecules while the hydrophobic groups retain contaminants. This is an ideal strategy that minimizes the product loss commonly experienced with HIC media due to denaturation of proteins upon exposure to hydrophobic surfaces.

More Information

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO 9001-registered corporation.

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1580090
25 ml, t-butyl hydrophobic interaction chromatography media, 50 μm particle size
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1560090
100 ml, t-butyl hydrophobic interaction chromatography media, 50 μm particle size
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1560091
500 ml, t-butyl hydrophobic interaction chromatography media, 50 μm particle size
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156-0092
5 L, t-butyl hydrophobic interaction chromatography media, 50 μm particle size
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156-0093
10 L, t-butyl hydrophobic interaction chromatography media, 50 μm particle size

Kits & Reagents

Kits & Reagents

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(Discontinued) Media Sampler Pack
1580100
Includes 25 ml each of UNOsphere™ Q and UNOsphere S, Macro-Prep® DEAE, Macro-Prep High Q, and Macro-Prep High S; 5 ml of UNOsphere SUPrA™; 10 g each of CHT™ Ceramic Hydroxyapatite Types I and II, 40 μm; and 10 g of CFT™ Ceramic Fluoroapatite Type II, 40 μm

Documents

Documents

Number Description Options
LIT486
Instruction Manual, Macro-Prep® HIC Supports, Rev C
1841
Macro-Prep® HIC Resin Product Information Sheet, Ver F
6792
Aggregate Purification Resin Selection Card, Ver A
6983
Native and Recombinant Protein Purification Resins/Media Selection Card
6808
Practical Guide: Selecting the Optimal Resins for Aggregate Removal, Ver A
6810
Practical Guide: Selecting the Optimal Resins for the Process Purification of Native and Recombinant Proteins, Ver A
6868
Macro-Prep® Chromatography Resins Publications List, Ver A
5662
Process Chromatography Applications Laboratory Brochure, Rev B