Macro-Prep Hydrophobic Interaction Chromatography (HIC) Resins are methacrylate-based, 50 µm beads used for protein, polypeptide, enzyme, and nucleic acid purification. They are designed specifically for intermediate purification steps that remove host-cell contaminants from partially purified targets. Due to their rigidity and unique surface chemistry, these hydrophobic chromatography resins are particularly suited for HIC operations requiring high throughput and high recovery of target.
Macro-Prep HIC Resin is available in two functional forms:
- Macro-Prep Methyl HIC Resin — weakly hydrophobic, for purifying compounds that have strong hydrophobic regions
- Macro-Prep t-Butyl HIC Resin — mildly hydrophobic, for purifying compounds that have few or weakly hydrophobic regions
Both HIC resins are chemically and thermally stable. These media are autoclavable and can withstand treatment in acid, chaotropic agents, or detergents while retaining high protein-binding capacities.
Macro-Prep t-Butyl HIC Resin operates on an interaction mechanism based on hydrophobicity and charge. The t-butyl groups are mildly hydrophobic. Depending on the pH of loading and elution buffers, the carboxyl groups can be exploited to ionically repel target molecules while the hydrophobic groups retain contaminants. This is an ideal strategy that minimizes the product loss commonly experienced with HIC media due to denaturation of proteins upon exposure to hydrophobic surfaces.
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