High-Fidelity PCR Kits

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Overview

High-fidelity PCR reagents featuring the thermostable iProof Polymerase. This enzyme utilizes Sso7d fusion polymerase technology to provide highly accurate amplification of targets up to 37 kb.

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Description

Description

iProof High-Fidelity DNA Polymerase is a unique Pyrococcus-like proofreading enzyme fused to the Sso7d dsDNA-binding protein to create a thermostable fusion polymerase that accurately amplifies long products from a variety of DNA templates.

Features and Benefits of iProof High-Fidelity DNA Polymerase:

  • High fidelity — iProof DNA polymerase is 52-fold more accurate than Taq polymerase
  • Speed — high processivity dramatically reduces extension (15–30 sec/kb) and overall reaction times
  • Successful amplification of long products with higher yields — fragments up to 37 kb are amplified in less time and with less enzyme (0.25–1 unit/reaction)

Choose the best DNA polymerase for your application.

Key Features iProof DNA Polymerase iTaq DNA Polymerase
Speed

Processivity

Long fragment amplification

Proofreading Yes No
Hot-Start No Yes
 

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Supporting Documentation

Specifications

Specifications

Storage at –20°C
iProof™ Enzyme, Kit, and Buffers: guaranteed for 6 months at –20°C in a constant-temperature freezer
iProof Master Mixes: guaranteed for 3 months at –20°C in a constant-temperature freezer (multiple freeze/thaws not recommended)
Storage at +4°C
Guaranteed for 3 months at 4°C

Ordering

Ordering

items

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1725310
100 x 50 µl reactions, premixed PCR reagents, includes 2x master mix (0.04 U/µl), DMSO; suitable for most templates
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1725320
100 x 50 µl reactions, premixed PCR reagents, includes 2x master mix (0.04 U/µl), DMSO; suitable for GC-rich templates
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1725300
20 U, 2 U/µl, stand-alone enzyme, includes 5x reaction buffers, MgCl2 solution, DMSO
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1725301
100 U, 2 U/µl, stand-alone enzyme, includes 5x reaction buffers, MgCl2 solution, DMSO
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1725302
500 U, 2 U/µl, stand-alone enzyme, includes 5x reaction buffers, MgCl2 solution, DMSO
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1725330
50 U, 2 U/µl, iProof polymerase PCR kit includes 5x reaction buffers, MgCl2 solution, DMSO, dNTPs, λ DNA, 1.3 and 10 kb primers, DNA standard
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1725331
200 U, 2 U/µl, iProof DNA polymerase PCR kit includes 5x reaction buffers, MgCl2 solution, DMSO, dNTPs, λ DNA, 1.3 and 10 kb primers, DNA standard

Documents

Documents

Number Description Options
10002300
Instruction Manual, iProof High-Fidelity PCR Kit, Rev B
10002298
Instruction Manual, iProof High-Fidelity DNA Polymerase, Rev B
5211
iProof High-Fidelity DNA Polymerase Flier, Rev C
[ Add to Cart (Free) ]
6252
Reagent Comparison Guide for Real-Time PCR Brochure, Rev C
[ Add to Cart (Free) ]
6090
Amplification Reagents and Plastics Brochure
[ Add to Cart (Free) ]
5337
Long Inverse PCR Using iProof Polymerase, Rev A
10000083948
Instruction Manual, iProof™ High-Fidelity PCR Master Mix