Gene Transfer/Transfection

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Overview

Introducing DNA and RNA into cells is a powerful tool for evaluating gene expression. Bio-Rad's products offer choices for transfection to bacterial, plant, fungal, and animal cells.
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Description

Description

Introducing DNA and RNA into cells is a powerful tool for evaluating gene expression. Bio-Rad's transfection products offer choices for gene transfer to bacterial, plant, fungal, and animal cells.

MicroPulser™ Electroporator for Microorganisms
The MicroPulser Electroporator is a simple and versatile electroporator that enables safe and reproducible transformation of bacteria, yeast, and other microorganisms.

Key features:

  • Simple one-button pulse delivery, attached cuvette chamber, and rapid charge time
  • Preset optimized programs for commonly studied bacteria and fungi to facilitate rapid program selection
  • Broad range of parameters for manual program optimization: voltage range of 200–3,000 V with 10 V precision and pulse range of 1.0–4.0 ms with 0.1 ms precision
  • 3,000 V capacity yields improved efficiency in larger volume cuvettes
  • Audible and visual pulse indicators

Gene Pulser®/Micropulser™ Electroporation Cuvettes
High-quality electroporation cuvettes are essential for consistent pulse delivery and reproducible results with your valuable samples. The precision pulse output of Bio-Rad's MicroPulser Electroporator is matched by high-quality electroporation cuvette construction. Bio-Rad's cuvettes are thoroughly washed to eliminate trace-inhibitors, then sterilized and individually wrapped.

TransFectin™ Reagent
TransFectin Lipid Reagent is a cationic lipid reagent that delivers the highest efficiencies and expression levels over a broad range of cell lines, including some difficult-to-transfect and primary cells, while minimizing damaging cytotoxic effects observed with other high-efficiency general transfection reagents. It provides high transfection efficiencies with lower amounts of lipid than most other lipid reagents, allowing more experiments per vial of reagent and lower costs per transfection.

Ordering

Ordering

items

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1652100EDU
100–120 V/220–240 V, electroporation instrument, includes 10 sterile cuvettes (5 each of 0.1 cm and 0.2 cm electrode gap width); education use only
165-2081EDU_tn.jpg
1652081EDU
Pkg of 5, 0.4 cm gap electroporation cuvette, for use with Gene Pulser and MicroPulser systems, for eukaryotic cells; education use only
165-2082EDU_tn.jpg
1652082EDU
Pkg of 5, 0.2 cm gap electroporation cuvette, for use with Gene Pulser and MicroPulser systems for yeast, bacteria, eukaryotic cells; education use only
165-2083EDU_tn.jpg
1652083EDU
Pkg of 5, 0.1 cm gap electroporation cuvette, for use with Gene Pulser and MicroPulser systems, for yeast and bacteria; education use only

Kits & Reagents

Kits & Reagents

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1703350EDU
0.5 ml, 1.5 mg/ml lipid transfection reagent, for applications including plasmid DNA, siRNA, and shRNA delivery; education use only
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1703351EDU
1 ml, 1.5 mg/ml lipid transfection reagent, for applications including plasmid DNA, siRNA, and shRNA delivery; education use only
170-3352_thumb.jpg
1703352EDU
5 ml (5 x 1 ml), 1.5 mg/ml lipid transfection reagent, for applications including plasmid DNA, siRNA, and shRNA delivery; education use only

Documents

Documents

Number Description Options
1908
Electroporation Cuvette Flier, Rev B
4006174
Instruction Manual, MicroPulser Electroporation Apparatus, Rev B
5448
MicroPulser Electroporator Flier, Rev A
2751
MicroPulser™ Electroporator Brochure, Rev A
5542
Electroporation Systems Brochure, Rev A
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