Gene Transfer/Transfection

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Gene Transfer/Transfection

Overview

Introducing DNA and RNA into cells is a powerful tool for evaluating gene expression. Bio-Rad's products offer choices for transfection to bacterial, plant, fungal, and animal cells.
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Description

Introducing DNA and RNA into cells is a powerful tool for evaluating gene expression. Bio-Rad's transfection products offer choices for gene transfer to bacterial, plant, fungal, and animal cells.

MicroPulser™ Electroporator for Microorganisms
The MicroPulser Electroporator is a simple and versatile electroporator that enables safe and reproducible transformation of bacteria, yeast, and other microorganisms.

Key features:

  • Simple one-button pulse delivery, attached cuvette chamber, and rapid charge time
  • Preset optimized programs for commonly studied bacteria and fungi to facilitate rapid program selection
  • Broad range of parameters for manual program optimization: voltage range of 200–3,000 V with 10 V precision and pulse range of 1.0–4.0 ms with 0.1 ms precision
  • 3,000 V capacity yields improved efficiency in larger volume cuvettes
  • Audible and visual pulse indicators

Gene Pulser®/Micropulser™ Electroporation Cuvettes
High-quality electroporation cuvettes are essential for consistent pulse delivery and reproducible results with your valuable samples. The precision pulse output of Bio-Rad's MicroPulser Electroporator is matched by high-quality electroporation cuvette construction. Bio-Rad's cuvettes are thoroughly washed to eliminate trace-inhibitors, then sterilized and individually wrapped.

TransFectin™ Reagent
TransFectin Lipid Reagent is a cationic lipid reagent that delivers the highest efficiencies and expression levels over a broad range of cell lines, including some difficult-to-transfect and primary cells, while minimizing damaging cytotoxic effects observed with other high-efficiency general transfection reagents. It provides high transfection efficiencies with lower amounts of lipid than most other lipid reagents, allowing more experiments per vial of reagent and lower costs per transfection.

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MicroPulser Electroporator

1652100EDU
100–120 V/220–240 V, electroporation instrument, includes 10 sterile cuvettes (5 each of 0.1 cm and 0.2 cm electrode gap width); education use only

0 1652100EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Gene-Transfer/165-2100EDU_0921121019117.xml 1652100EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jthvlbdb 2017-11-14 07:07:19 true true internet/product_SKU jthvlbdb templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Gene-Transfer/165-2100EDU_0921121019117.xml LSE 2019-05-04 11:13:13 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972096307 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 11:13:13 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Gene-Transfer/165-2100EDU_0921121019117.xml templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Gene-Transfer/165-2100EDU_0921121019117.xml 1652100edu-micropulser-electroporator 1652100EDU MicroPulser Electroporator MicroPulser Electroporator 100–120 V/220–240 V, electroporation instrument, includes 10 sterile cuvettes (5 each of 0.1 cm and 0.2 cm electrode gap width); education use only 100–120 V/220–240 V, electroporation instrument, includes 10 sterile cuvettes (5 each of 0.1 cm and 0.2 cm electrode gap width); education use only 100–120 V/220–240 V, electroporation instrument, includes 10 sterile cuvettes (5 each of 0.1 cm and 0.2 cm electrode gap width); education use only /webroot/web/images/lse/products/equipment_supplies/sku_thumb/global/165-2100EDU_tn.jpg /webroot/web/images/lse/products/equipment_supplies/sku_view/global/165-2100EDU_view.jpg /webroot/web/images/lse/products/equipment_supplies/sku_feature/global/165-2100EDU_feature.jpg RepEx 165-2100EDU 100–120 V/220–240 V, electroporation instrument, includes 10 sterile cuvettes (5 each of 0.1 cm and 0.2 cm electrode gap width); education use only 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Equipment-and-Supplies/Gene-Transfer N 0 1652100, 1652100EDU, micropulser, electroporator, electroporation, instrument, gene, delivery, transfection, transformation, science, education, products, classroom false $2,621.00 true $ %241%2C965.75 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1652100EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1652100EDU&CartQuote=QT&quantity= 1715 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1652100EDU&AddToDefaultWishList=true&quantity= 102003000 2621.00 Product detail page 1652100EDU
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Gene Pulser/MicroPulser Cuvette

1652081EDU
Pkg of 5, 0.4 cm gap electroporation cuvette, for use with Gene Pulser and MicroPulser systems, for eukaryotic cells; education use only

1 1652081EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Gene-Transfer/165-2081EDU_0921121019178.xml 1652081EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jthvlb95 2017-11-14 07:07:19 true true internet/product_SKU jthvlb95 templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Gene-Transfer/165-2081EDU_0921121019178.xml LSE 2019-05-04 11:12:46 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972096140 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 11:12:46 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Gene-Transfer/165-2081EDU_0921121019178.xml templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Gene-Transfer/165-2081EDU_0921121019178.xml 1652081edu-gene-pulser-micropulser-cuvette 1652081EDU Gene Pulser/MicroPulser Cuvette Gene Pulser/MicroPulser Cuvette Pkg of 5, 0.4 cm gap electroporation cuvette, for use with Gene Pulser and MicroPulser systems, for eukaryotic cells; education use only Pkg of 5, 0.4 cm gap electroporation cuvette, for use with Gene Pulser and MicroPulser systems, for eukaryotic cells; education use only Pkg of 5, 0.4 cm gap electroporation cuvette, for use with Gene Pulser and MicroPulser systems, for eukaryotic cells; education use only /webroot/web/images/lse/products/equipment_supplies/sku_thumb/global/165-2081EDU_tn.jpg /webroot/web/images/lse/products/equipment_supplies/sku_view/global/165-2081EDU_view.jpg /webroot/web/images/lse/products/equipment_supplies/sku_feature/global/165-2081EDU_feature.jpg RepEx 165-2081EDU Pkg of 5, 0.4 cm gap electroporation cuvette, for use with Gene Pulser and MicroPulser systems, for eukaryotic cells; education use only 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Equipment-and-Supplies/Gene-Transfer N 0 165-2081EDU, 1652081EDU, gene, pulser/micropulser, electroporation, cuvettes, 0.4, gap, pulser, micropulser, electroporator, mammalian, transfection, transfer, delivery, cells, plant, protoplasts, nucleofection, primary, stem, eukaryotic, science, education, products 1652676 /templatedata/internet/product_SKU/data/LSR/Transfection/Electroporation/165-2676_0921120525234.xml 1652660 /templatedata/internet/product_SKU/data/LSR/Transfection/Electroporation/165-2660_0921120525407.xml 1703361 /templatedata/internet/product_SKU/data/LSR/Transfection/Lipid-Transfection/170-3361_0921120516973.xml false $15.00 true $ %2412.00 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1652081EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1652081EDU&CartQuote=QT&quantity= 1717 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1652081EDU&AddToDefaultWishList=true&quantity= 41100000 15.00 Product detail page 1652081EDU
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Gene Pulser/MicroPulser Cuvette

1652082EDU
Pkg of 5, 0.2 cm gap electroporation cuvette, for use with Gene Pulser and MicroPulser systems for yeast, bacteria, eukaryotic cells; education use only

2 1652082EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Gene-Transfer/165-2082EDU_0921121019158.xml 1652082EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jthvlbb7 2017-11-14 07:07:19 true true internet/product_SKU jthvlbb7 templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Gene-Transfer/165-2082EDU_0921121019158.xml LSE 2019-05-04 11:13:01 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972096223 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 11:13:01 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Gene-Transfer/165-2082EDU_0921121019158.xml templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Gene-Transfer/165-2082EDU_0921121019158.xml 1652082edu-gene-pulser-micropulser-cuvette 1652082EDU Gene Pulser/MicroPulser Cuvette Gene Pulser/MicroPulser Cuvette Pkg of 5, 0.2 cm gap electroporation cuvette, for use with Gene Pulser and MicroPulser systems for yeast, bacteria, eukaryotic cells; education use only Pkg of 5, 0.2 cm gap electroporation cuvette, for use with Gene Pulser and MicroPulser systems for yeast, bacteria, eukaryotic cells; education use only Pkg of 5, 0.2 cm gap electroporation cuvette, for use with Gene Pulser and MicroPulser systems for yeast, bacteria, eukaryotic cells; education use only /webroot/web/images/lse/products/equipment_supplies/sku_thumb/global/165-2082EDU_tn.jpg /webroot/web/images/lse/products/equipment_supplies/sku_view/global/165-2082EDU_view.jpg /webroot/web/images/lse/products/equipment_supplies/sku_feature/global/165-2082EDU_feature.jpg RepEx 165-2082EDU Pkg of 5, 0.2 cm gap electroporation cuvette, for use with Gene Pulser and MicroPulser systems for yeast, bacteria, eukaryotic cells; education use only 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Equipment-and-Supplies/Gene-Transfer N 0 165-2082EDU, 1652082EDU, gene, pulser/micropulser, electroporation, cuvettes, 0.2, gap, pulser, micropulser, electroporator, yeast, bacteria, bacterial, microbial, mammalian, transformation, transfection, transfer, delivery, prokaryotic, cells, nucleofection, primary, stem, eukaryotic, science, education, products, classroom 1652676 /templatedata/internet/product_SKU/data/LSR/Transfection/Electroporation/165-2676_0921120525234.xml 1652660 /templatedata/internet/product_SKU/data/LSR/Transfection/Electroporation/165-2660_0921120525407.xml 1703361 /templatedata/internet/product_SKU/data/LSR/Transfection/Lipid-Transfection/170-3361_0921120516973.xml false $15.00 true $ %2412.00 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1652082EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1652082EDU&CartQuote=QT&quantity= 1717 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1652082EDU&AddToDefaultWishList=true&quantity= 41100000 15.00 Product detail page 1652082EDU
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Gene Pulser/MicroPulser Cuvette

1652083EDU
Pkg of 5, 0.1 cm gap electroporation cuvette, for use with Gene Pulser and MicroPulser systems, for yeast and bacteria; education use only

3 1652083EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Gene-Transfer/165-2083EDU_0921121019137.xml 1652083EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jthvlbes 2017-11-14 07:07:19 true true internet/product_SKU jthvlbes templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Gene-Transfer/165-2083EDU_0921121019137.xml LSE 2019-05-04 11:13:22 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972096265 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 11:13:22 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Gene-Transfer/165-2083EDU_0921121019137.xml templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Gene-Transfer/165-2083EDU_0921121019137.xml 1652083edu-gene-pulser-micropulser-cuvette 1652083EDU Gene Pulser/MicroPulser Cuvette Gene Pulser/MicroPulser Cuvette Pkg of 5, 0.1 cm gap electroporation cuvette, for use with Gene Pulser and MicroPulser systems, for yeast and bacteria; education use only Pkg of 5, 0.1 cm gap electroporation cuvette, for use with Gene Pulser and MicroPulser systems, for yeast and bacteria; education use only Pkg of 5, 0.1 cm gap electroporation cuvette, for use with Gene Pulser and MicroPulser systems, for yeast and bacteria; education use only /webroot/web/images/lse/products/equipment_supplies/sku_thumb/global/165-2083EDU_tn.jpg /webroot/web/images/lse/products/equipment_supplies/sku_view/global/165-2083EDU_view.jpg /webroot/web/images/lse/products/equipment_supplies/sku_feature/global/165-2083EDU_feature.jpg RepEx 165-2083EDU Pkg of 5, 0.1 cm gap electroporation cuvette, for use with Gene Pulser and MicroPulser systems, for yeast and bacteria; education use only 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Equipment-and-Supplies/Gene-Transfer N 0 165-2083EDU, 1652083EDU, gene, pulser/micropulser, electroporation, cuvettes, 0.1, gap, pulser, micropulser, electroporator, yeast, bacteria, bacterial, e., coli, microbial, transformation, transfer, delivery, prokaryotic, cells, science, education, products, classroom 1652676 /templatedata/internet/product_SKU/data/LSR/Transfection/Electroporation/165-2676_0921120525234.xml 1652660 /templatedata/internet/product_SKU/data/LSR/Transfection/Electroporation/165-2660_0921120525407.xml 1703361 /templatedata/internet/product_SKU/data/LSR/Transfection/Lipid-Transfection/170-3361_0921120516973.xml false $15.00 true $ %2412.00 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1652083EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1652083EDU&CartQuote=QT&quantity= 1717 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1652083EDU&AddToDefaultWishList=true&quantity= 41100000 15.00 Product detail page 1652083EDU
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TransFectin™ Lipid Reagent, 0.5 ml

1703350EDU
0.5 ml, 1.5 mg/ml lipid transfection reagent, for applications including plasmid DNA, siRNA, and shRNA delivery; education use only

1703350EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/1703350EDU_1451933910.xml 1703350EDU /default/main/bio-rad/WORKAREA/default 01/04/16 10:56 AM en_US juso9kdd 2018-02-01 00:09:37 true true internet/product_SKU juso9kdd templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/1703350EDU_1451933910.xml LSE 2019-05-04 10:29:25 /default/main/bio-rad/STAGING N /default/main/bio-rad en 01/04/26 10:56 AM 1555970222406 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 10:29:25 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/1703350EDU_1451933910.xml templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/1703350EDU_1451933910.xml 1703350edu-transfectin-lipid-reagent-0-5-ml 1703350EDU Transfectin Reagent, 0.5 ml TransFectin&trade; Lipid Reagent, 0.5 ml 0.5 ml, 1.5 mg/ml lipid transfection reagent, for applications including plasmid DNA, siRNA, and shRNA delivery; education use only /webroot/web/images/lsr/products/gene_transfer_rnai/sku_thumb/global/170-3350_thumb.jpg /webroot/web/images/lsr/products/gene_transfer_rnai/sku_view/global/170-3350_view.jpg TransFectin Lipid Reagent, 0.5 ml preview ,VN Molly Gregg TransFectin Lipid Reagent, 0.5 ml 1 ml, 1.5 mg/ml lipid transfection reagent, for applications including plasmid DNA, siRNA, and shRNA delivery; education use only 01/04/16 10:56 AM 01/04/26 10:56 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Equipment-and-Supplies/Reagents N 0 <p>Teach your students about transfection with a high rate of success. When paired with GFP lessons and ZOE class activities, you can simultaneously explain the principles of fluorescent imaging.</p> <p>Use the general purpose TransFectin Lipid Reagent for high-efficiency transfection of your cell lines or primary cells, including difficult-to-transfect cells.</p> <p>The proprietary mixture of cationic compound and the co-lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) is designed to provide high expression levels with reduced cytotoxicity.</p> <ul> <li> Sufficient for 60 to 100 cell transfections in 35 mm plates</li> <li> Simple 3-step protocol gives high-efficiency transfection in a broad range of cells with high levels of cell viability</li> <li> Transfection at culture densities from 40% to 90%</li> <li> Designed to work in media containing serum but can be used in the absence of serum</li> <li> No post transfection media changes required</li> <li> Polystyrene is recommended because cationic lipid-plasmid complexes may bind to polypropylene</li> </ul> <p>The <a href="http://biorad-ads.com/transfection_protocols/" target="_blank">Transfection Protocol Online Library</a> features a comprehensive list of cell lines and primary cultures and contains protocols from a range of sources for transfection using lipids, electroporation, and biolistic particle delivery.</p> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 300px;" border="0"> <tbody> <tr> <td style="text-align: center;" valign="top"><strong>Volume</strong></td> <td style="text-align: center;" valign="top"><strong>Number of Vials</strong></td> <td style="text-align: center;" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: center;" valign="top">0.5 ml</td> <td style="text-align: center;" valign="top">1 x 0.5 ml</td> <td style="text-align: center;" valign="top">1703350EDU</td> </tr> <tr> <td style="text-align: center;" valign="top">1 ml</td> <td style="text-align: center;" valign="top">1 x 1 ml</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1703351edu-transfectin-lipid-reagent-1-ml">1703351EDU</a></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: center;" valign="top">5 ml</td> <td style="text-align: center;" valign="top">5 x 1 ml</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1703352edu-transfectin-lipid-reagent-5-ml">1703352EDU</a></td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Related Products</h2> <ul> <li> <a href="/en-us/sku/1351201edu-cfda-se">CFDA-SE Dye</a> to monitor cell health</li> <li> <a href="/en-us/sku/1351111edu-vivafix-353-442-cell-viability-assay?ID=1351111EDU">Fixable Viability Dyes</a> to distinguish between live and dead cells</li> <li> <a href="/en-us/sku/1351303edu-pureblu-dapi-nuclear-staining-dye">PureBlu&trade; DAPI</a> and <a href="/en-us/sku/1351304edu-pureblu-hoechst-33342-nuclear-staining-dye">PureBlu Hoechst 33342</a> Nuclear Staining Dye to visualize nuclei</li> <li> <a href="/en-us/product/silentfect-lipid-reagent-for-rnai">siLentFect&trade; Lipid Reagent for RNAi</a> for delivery of siRNA alone or cotransfected with a small reporter-plasmid</li> <li> <a href="/en-us/product/smartspec-plus-spectrophotometer?pcp_loc=catprod">SmartSpec&trade; Plus Spectrophotometer</a></li> <li> <a href="/en-us/product/imaging/zoe-fluorescent-cell-imager">ZOE&trade; Fluorescent Cell Imager</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li> <a href="/en-us/product/gene-transfer-transfection">Gene Transfer/Transfection</a></li> <li> <a href="/en-us/category/biolistic-particle-delivery-systems">Biolistic Particle Delivery Systems</a></li> <li> <a href="/en-us/product/nucleic-acid-purification">Nucleic Acid Extraction and Purification</a></li> <li> <a href="/en-us/category/pcr-instrumentation">PCR Instrumentation</a></li> <li> <a href="/en-us/product/pcr-amplification-reagents ">PCR Amplification Reagents</a></li> </ul> <h2 class="descHeader">Supporting Documentation</h2> <ul> <li> Fluorescent Cell Imaging Activities for Your Classroom <a class="no-target-style" href="/webroot/web/pdf/lse/literature/Bulletin_6686.pdf" target="_blank">Flier</a></li> <li> TransFectin Lipid Reagent <a class="no-target-style" href="/webroot/web/pdf/lsr/literature/Bulletin_2873.pdf" target="_blank">Brochure</a></li> <li> TransFectin Lipid Reagent <a class="no-target-style" href="/webroot/web/pdf/lsr/literature/4106254A.pdf" target="_blank">Instruction Manual</a></li> <li> Optimization of TransFectin Lipid Reagent-Mediated Transfection for Different Cell Types <a class="no-target-style" href="/webroot/web/pdf/lsr/literature/Bulletin_3197.pdf" target="_blank">Bulletin</a></li> <li> Transfection Reagent <a class="no-target-style" href="/webroot/web/pdf/lsr/literature/Bulletin_5343C.pdf" target="_blank">References</a></li> </ul> <h2 class="descHeader">Online Applications</h2> <p><a href="/en-us/applications-technologies/introduction-transfection?ID=LUSONCB9O">Transfection</a></p> /webroot/web/html/lse/products/SKUs 1703350edu.html /webroot/web/html/lse/products/SKUs/1703350edu.html 1 0 false $219.00 true $ %24175.20 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703350EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703350EDU&CartQuote=QT&quantity= 1717 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703350EDU&AddToDefaultWishList=true&quantity= 12161500 219.00 Product detail page 1703350EDU
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TransFectin™ Lipid Reagent, 1 ml

1703351EDU
1 ml, 1.5 mg/ml lipid transfection reagent, for applications including plasmid DNA, siRNA, and shRNA delivery; education use only

1703351EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/1703351EDU_1451934008.xml 1703351EDU /default/main/bio-rad/WORKAREA/default 01/04/16 10:58 AM en_US juso9kdf 2018-02-01 00:09:37 true true internet/product_SKU juso9kdf templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/1703351EDU_1451934008.xml LSE 2019-05-04 10:30:05 /default/main/bio-rad/STAGING N /default/main/bio-rad en 01/04/26 10:59 AM 1555970222519 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 10:30:05 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/1703351EDU_1451934008.xml templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/1703351EDU_1451934008.xml 1703351edu-transfectin-lipid-reagent-1-ml 1703351EDU Transfectin Reagent, 1 ml TransFectin&trade; Lipid Reagent, 1 ml 1 ml, 1.5 mg/ml lipid transfection reagent, for applications including plasmid DNA, siRNA, and shRNA delivery; education use only /webroot/web/images/lsr/products/gene_transfer_rnai/sku_thumb/global/170-3351_thumb.jpg /webroot/web/images/lsr/products/gene_transfer_rnai/sku_view/global/170-3351_view.jpg preview ,VN Molly Gregg TransFectin Lipid Reagent, 1 ml 1 ml, 1.5 mg/ml lipid transfection reagent, for applications including plasmid DNA, siRNA, and shRNA delivery; education use only 01/04/16 10:58 AM 01/04/26 10:59 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Equipment-and-Supplies/Reagents N 0 <p>Teach your students about transfection with a high rate of success. When paired with GFP lessons and ZOE class activities, you can simultaneously explain the principles of fluorescent imaging.</p> <p>Use the general purpose TransFectin Lipid Reagent for high-efficiency transfection of your cell lines or primary cells, including difficult-to-transfect cells.</p> <p>The proprietary mixture of cationic compound and the co-lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) is designed to provide high expression levels with reduced cytotoxicity.</p> <ul> <li> Sufficient for 60 to 100 cell transfections in 35 mm plates</li> <li> Simple 3-step protocol gives high-efficiency transfection in a broad range of cells with high levels of cell viability</li> <li> Transfection at culture densities from 40% to 90%</li> <li> Designed to work in media containing serum but can be used in the absence of serum</li> <li> No post transfection media changes required</li> <li> Polystyrene is recommended because cationic lipid-plasmid complexes may bind to polypropylene</li> </ul> <p>The <a href="http://biorad-ads.com/transfection_protocols/" target="_blank">Transfection Protocol Online Library</a> features a comprehensive list of cell lines and primary cultures and contains protocols from a range of sources for transfection using lipids, electroporation, and biolistic particle delivery.</p> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 300px;" border="0"> <tbody> <tr> <td style="text-align: center;" valign="top"><strong>Volume</strong></td> <td style="text-align: center;" valign="top"><strong>Number of Vials</strong></td> <td style="text-align: center;" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: center;" valign="top">0.5 ml</td> <td style="text-align: center;" valign="top">1 x 0.5 ml</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1703350edu-transfectin-lipid-reagent-0-5-ml">1703350EDU</a></td> </tr> <tr> <td style="text-align: center;" valign="top">1 ml</td> <td style="text-align: center;" valign="top">1 x 1 ml</td> <td style="text-align: center;" valign="top">1703351EDU</td> </tr> <tr class="pd_colorbackground"> <td style="text-align: center;" valign="top">5 ml</td> <td style="text-align: center;" valign="top">5 x 1 ml</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1703352edu-transfectin-lipid-reagent-5-ml">1703352EDU</a></td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Related Products</h2> <ul> <li> <a href="/en-us/sku/1351201edu-cfda-se">CFDA-SE Dye</a> to monitor cell health</li> <li> <a href="/en-us/sku/1351111edu-vivafix-353-442-cell-viability-assay?ID=1351111EDU">Fixable Viability Dyes</a> to distinguish between live and dead cells</li> <li> <a href="/en-us/sku/1351303edu-pureblu-dapi-nuclear-staining-dye">PureBlu&trade; DAPI</a> and <a href="/en-us/sku/1351304edu-pureblu-hoechst-33342-nuclear-staining-dye">PureBlu Hoechst 33342</a> Nuclear Staining Dye to visualize nuclei</li> <li> <a href="/en-us/product/silentfect-lipid-reagent-for-rnai">siLentFect&trade; Lipid Reagent for RNAi</a> for delivery of siRNA alone or cotransfected with a small reporter-plasmid</li> <li> <a href="/en-us/product/smartspec-plus-spectrophotometer?pcp_loc=catprod">SmartSpec&trade; Plus Spectrophotometer</a></li> <li> <a href="/en-us/product/imaging/zoe-fluorescent-cell-imager">ZOE&trade; Fluorescent Cell Imager</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li> <a href="/en-us/product/gene-transfer-transfection">Gene Transfer/Transfection</a></li> <li> <a href="/en-us/category/biolistic-particle-delivery-systems">Biolistic Particle Delivery Systems</a></li> <li> <a href="/en-us/product/nucleic-acid-purification">Nucleic Acid Extraction and Purification</a></li> <li> <a href="/en-us/category/pcr-instrumentation">PCR Instrumentation</a></li> <li> <a href="/en-us/product/pcr-amplification-reagents ">PCR Amplification Reagents</a></li> </ul> <h2 class="descHeader">Supporting Documentation</h2> <ul> <li> Fluorescent Cell Imaging Activities for Your Classroom <a class="no-target-style" href="/webroot/web/pdf/lse/literature/Bulletin_6686.pdf" target="_blank">Flier</a></li> <li> TransFectin Lipid Reagent <a class="no-target-style" href="/webroot/web/pdf/lsr/literature/Bulletin_2873.pdf" target="_blank">Brochure</a></li> <li> TransFectin Lipid Reagent <a class="no-target-style" href="/webroot/web/pdf/lsr/literature/4106254A.pdf" target="_blank">Instruction Manual</a></li> <li> Optimization of TransFectin Lipid Reagent-Mediated Transfection for Different Cell Types <a class="no-target-style" href="/webroot/web/pdf/lsr/literature/Bulletin_3197.pdf" target="_blank">Bulletin</a></li> <li> Transfection Reagent <a class="no-target-style" href="/webroot/web/pdf/lsr/literature/Bulletin_5343C.pdf" target="_blank">References</a></li> </ul> <h2 class="descHeader">Online Applications</h2> <p><a href="/en-us/applications-technologies/introduction-transfection?ID=LUSONCB9O">Transfection</a></p> /webroot/web/html/lse/products/SKUs 1703351edu.html /webroot/web/html/lse/products/SKUs/1703351edu.html 1 0 false $411.00 true $ %24328.80 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703351EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703351EDU&CartQuote=QT&quantity= 1717 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703351EDU&AddToDefaultWishList=true&quantity= 12161500 411.00 Product detail page 1703351EDU
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TransFectin™ Lipid Reagent, 5 ml

1703352EDU
5 ml (5 x 1 ml), 1.5 mg/ml lipid transfection reagent, for applications including plasmid DNA, siRNA, and shRNA delivery; education use only

1703352EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/1703352EDU_1451934105.xml 1703352EDU /default/main/bio-rad/WORKAREA/default 01/04/16 11:00 AM en_US juso9kdb 2018-02-01 00:09:37 true true internet/product_SKU juso9kdb templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/1703352EDU_1451934105.xml LSE 2019-05-04 10:29:21 /default/main/bio-rad/STAGING N /default/main/bio-rad en 01/04/26 11:01 AM 1555970222562 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 10:29:21 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/1703352EDU_1451934105.xml templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/1703352EDU_1451934105.xml 1703352edu-transfectin-lipid-reagent-5-ml 1703352EDU Transfectin Reagent, 5 ml TransFectin&trade; Lipid Reagent, 5 ml 5 ml (5 x 1 ml), 1.5 mg/ml lipid transfection reagent, for applications including plasmid DNA, siRNA, and shRNA delivery; education use only /webroot/web/images/lsr/products/gene_transfer_rnai/sku_thumb/global/170-3352_thumb.jpg /webroot/web/images/lsr/products/gene_transfer_rnai/sku_view/global/170-3352_view.jpg preview ,VN Molly Gregg TransFectin Lipid Reagent, 5 ml 5 ml (5 x 1 ml), 1.5 mg/ml lipid transfection reagent, for applications including plasmid DNA, siRNA, and shRNA delivery; education use only 01/04/16 11:00 AM 01/04/26 11:01 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Equipment-and-Supplies/Reagents N 0 <p>Teach your students about transfection with a high rate of success. When paired with GFP lessons and ZOE class activities, you can simultaneously explain the principles of fluorescent imaging.</p> <p>Use the general purpose TransFectin Lipid Reagent for high-efficiency transfection of your cell lines or primary cells, including difficult-to-transfect cells.</p> <p>The proprietary mixture of cationic compound and the co-lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) is designed to provide high expression levels with reduced cytotoxicity.</p> <ul> <li> Sufficient for 60 to 100 cell transfections in 35 mm plates</li> <li> Simple 3-step protocol gives high-efficiency transfection in a broad range of cells with high levels of cell viability</li> <li> Transfection at culture densities from 40% to 90%</li> <li> Designed to work in media containing serum but can be used in the absence of serum</li> <li> No post transfection media changes required</li> <li> Polystyrene is recommended because cationic lipid-plasmid complexes may bind to polypropylene</li> </ul> <p>The <a href="http://biorad-ads.com/transfection_protocols/" target="_blank">Transfection Protocol Online Library</a> features a comprehensive list of cell lines and primary cultures and contains protocols from a range of sources for transfection using lipids, electroporation, and biolistic particle delivery.</p> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 300px;" border="0"> <tbody> <tr> <td style="text-align: center;" valign="top"><strong>Volume</strong></td> <td style="text-align: center;" valign="top"><strong>Number of Vials</strong></td> <td style="text-align: center;" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: center;" valign="top">0.5 ml</td> <td style="text-align: center;" valign="top">1 x 0.5 ml</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1703350edu-transfectin-lipid-reagent-0-5-ml">1703350EDU</a></td> </tr> <tr> <td style="text-align: center;" valign="top">1 ml</td> <td style="text-align: center;" valign="top">1 x 1 ml</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1703351edu-transfectin-lipid-reagent-1-ml">1703351EDU</a></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: center;" valign="top">5 ml</td> <td style="text-align: center;" valign="top">5 x 1 ml</td> <td style="text-align: center;" valign="top">1703352EDU</td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Related Products</h2> <ul> <li> <a href="/en-us/sku/1351201edu-cfda-se">CFDA-SE Dye</a> to monitor cell health</li> <li> <a href="/en-us/sku/1351111edu-vivafix-353-442-cell-viability-assay?ID=1351111EDU">Fixable Viability Dyes</a> to distinguish between live and dead cells</li> <li> <a href="/en-us/sku/1351303edu-pureblu-dapi-nuclear-staining-dye">PureBlu&trade; DAPI</a> and <a href="/en-us/sku/1351304edu-pureblu-hoechst-33342-nuclear-staining-dye">PureBlu Hoechst 33342</a> Nuclear Staining Dye to visualize nuclei</li> <li> <a href="/en-us/product/silentfect-lipid-reagent-for-rnai">siLentFect&trade; Lipid Reagent for RNAi</a> for delivery of siRNA alone or cotransfected with a small reporter-plasmid</li> <li> <a href="/en-us/product/smartspec-plus-spectrophotometer?pcp_loc=catprod">SmartSpec&trade; Plus Spectrophotometer</a></li> <li> <a href="/en-us/product/imaging/zoe-fluorescent-cell-imager">ZOE&trade; Fluorescent Cell Imager</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li> <a href="/en-us/product/gene-transfer-transfection">Gene Transfer/Transfection</a></li> <li> <a href="/en-us/category/biolistic-particle-delivery-systems">Biolistic Particle Delivery Systems</a></li> <li> <a href="/en-us/product/nucleic-acid-purification">Nucleic Acid Extraction and Purification</a></li> <li> <a href="/en-us/category/pcr-instrumentation">PCR Instrumentation</a></li> <li> <a href="/en-us/product/pcr-amplification-reagents ">PCR Amplification Reagents</a></li> </ul> <h2 class="descHeader">Supporting Documentation</h2> <ul> <li> Fluorescent Cell Imaging Activities for Your Classroom <a class="no-target-style" href="/webroot/web/pdf/lse/literature/Bulletin_6686.pdf" target="_blank">Flier</a></li> <li> TransFectin Lipid Reagent <a class="no-target-style" href="/webroot/web/pdf/lsr/literature/Bulletin_2873.pdf" target="_blank">Brochure</a></li> <li> TransFectin Lipid Reagent <a class="no-target-style" href="/webroot/web/pdf/lsr/literature/4106254A.pdf" target="_blank">Instruction Manual</a></li> <li> Optimization of TransFectin Lipid Reagent-Mediated Transfection for Different Cell Types <a class="no-target-style" href="/webroot/web/pdf/lsr/literature/Bulletin_3197.pdf" target="_blank">Bulletin</a></li> <li> Transfection Reagent <a class="no-target-style" href="/webroot/web/pdf/lsr/literature/Bulletin_5343C.pdf" target="_blank">References</a></li> </ul> <h2 class="descHeader">Online Applications</h2> <p><a href="/en-us/applications-technologies/introduction-transfection?ID=LUSONCB9O">Transfection</a></p> /webroot/web/html/lse/products/SKUs 1703352edu.html /webroot/web/html/lse/products/SKUs/ 1703352edu.html 1 0 false $1,520.00 true $ %241%2C216.00 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703352EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703352EDU&CartQuote=QT&quantity= 1717 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703352EDU&AddToDefaultWishList=true&quantity= 12161500 1520.00 Product detail page 1703352EDU
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