ddPCR Multiplex Mutation Screening Kits
Droplet Digital PCR (ddPCR) Multiplex Mutation Screening Kits are designed for rapid screening of several key cancer mutations in a single reaction with high precision and sensitivity. These kits are ideal for use with formalin-fixed, paraffin-embedded (FFPE) samples, liquid biopsy, fresh/frozen tissue, and samples with low yield or inhibitory substances. The kits contain an optimized, validated ddPCR Mutation Screening Multiplex Assay and ddPCR Supermix for Probes (No dUTP).
Overview
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Droplet Digital PCR (ddPCR) Multiplex Mutation Screening Kits are designed for rapid screening of several key cancer mutations in a single reaction with high precision and sensitivity. These kits are ideal for use with formalin-fixed, paraffin-embedded (FFPE) samples, liquid biopsy, fresh/frozen tissue, and samples with low yield or inhibitory substances. The kits contain an optimized, validated ddPCR Mutation Screening Multiplex Assay and ddPCR Supermix for Probes (No dUTP).
Key Benefits
- Screen multiple mutations in a single well
- Detect with high sensitivity (limit of detection ≤0.5%)
- Process numerous samples in a rapid, cost-effective manner
- Work with low amounts of input DNA without preamplification
- Use with Bio-Rad’s QX100, QX200, or QX200 AutoDG Systems
Mutations Detected by ddPCR Multiplex Mutation Screening Kits
| Catalog # | ddPCR Multiplex Mutation Screening Kit* | Mutations Detected |
|---|---|---|
| 12015172 | MSI** | BAT25/26, NR21/24, Mono27 |
| 12001037 | BRAF V600 | V600E, V600K, V600R |
| 1863506 | KRAS G12/G13 | G12A, G12C, G12D, G12R, G12S, G12V, G13D |
| 12001626 | KRAS Q61 | Q61H (183A>C), Q61H (183A>T), Q61K, Q61L, Q61R |
| 12001094 | NRAS G12 | G12A, G12C, G12D, G12R, G12S, G12V |
| 12001627 | NRAS G12/G13 | G12A, G12C, G12D, G12S, G12V, G13D, G13R, G13V |
| 12001006 | NRAS Q61 | Q61H (183A>C), Q61H (183A>T), Q61K, Q61L, Q61R |
| 12002392 | EGFR exon19 deletions | >15 EGFR exon19 deletions |
*Assays for each of the mutations detected by these kits can also be ordered individually. Each kit contains 20x ddPCR Mutation Screening Multiplex Assay (1 x 200 μl) and 2x ddPCR Supermix for Probes (No dUTP) (2 x 1 ml).
**Each kit contains ddPCR Multiplex Supermix (2x 600 ul), ddPCR MSI Assay 1 (BAT25/26), ddPCR MSI Assay 2 ((NR21/24), ddPCR Assay 3 (Mono27), Uracil-DNA Glycosylase (UDG), positive control, and nuclease-free water.
Key Actionable Mutations in Various Cancers
| Gene | Lung Cancer | Colorectal Cancer | Ovarian Cancer | Thyroid Cancer | Melanoma | GIST | Glioma | Pancreatic Cancer |
|---|---|---|---|---|---|---|---|---|
| BRAF | • | • | • | • | • | • | • | |
| KRAS | • | • | • | • | • | |||
| NRAS | • | • | • | • | ||||
| EGFR | • | • | • | |||||
| BAT25/BAT26 | • | |||||||
| NR21/NR24 | • | |||||||
| Mono27 | • |
GIST, gastrointestinal stromal tumor.
Resources
Droplet Digital PCR Applications Guide
Read in-depth information about setting up experiments for ddPCR applications.
Rare Mutation Detection Best Practices Guidelines
Learn best practices for performing mutation detection experiments.
Ordering
200 x 20 ul reactions, includes ddPCR MSI Assay 1 (BAT25/26), MSI Assay 2 (NR21/24), MSI Assay 3 (Mon27), ddPCR Multiplex Supermix, Uracil-DNA Glycosylase (UDG), positive control, and nuclease-free water for screening mutations in 5 MSI markers using the QX200, QX600, QX200 AutoDG, or QX600 Auto
