dd-Check STEC Solution

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dd-Check STEC Solution

Overview

dd-Check STEC kit provides a way to detect and confirm linked virluence genes and allows descrimination between true positive and true negative EHEC samples.
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  • Description
  • Ordering
  • Accessories
  • Equipment
  • Documents

Description

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The dd-Check STEC Solution uses Droplet Digital PCR technology to detect double-positive linked virulence genes (stx and eae) in a single bacterium from samples containing bacteria with single-positive or unlinked virulence genes, reducing the number of false positive Shiga toxin-producing Escherichia coli (STEC) results. This detection and linkage verification of targets in a single bacterium enhances the accuracy of pathogenic E. coli testing.

Features and Benefits

  • Less false positive results saves time and money with less unnecessary confirmations
  • Confidence in results
  • Decreasing time to results of screening and confirmation allows product to go to market faster
  • Comply with regulations that classify STEC as an adulterant in food and keep up with changes in regulations

Applications and Uses

  • Test for the detection and colocalization of virulence genes in Shiga toxin-producing Escherichia coli (STEC)

Data Analysis

The Qx IDE Manager Software — the optimal software for data analysis — was designed specifically for food safety applications. It provides complete automated analysis for the dd-Check STEC Assay. Results interpretation is based on the presence or absence of the two targets (stx and eae) and on the linkage analysis showing the colocalization of these virulence genes coming from a single bacterium.

dd-Check STEC Workflow

The dd-Check STEC Workflow is scalable to allow users the flexibility to test 1 to 94 samples per plate. Laboratories can use the dd-Check STEC Solution as an initial screening tool or after screening with the iQ-Check STEC Kits for next-day confirmation of pathogenic E. coli.


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dd-Check STEC Solution

17004826
Test for the detection and colocalization of virulence genes in Shiga toxin-producing Escherichia coli (STEC)

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dd-Check STEC Droplet Generation Oil

12010000
7ml, oil use with the QX200 Droplet Generator

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$52.00 
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dd-Check STEC Kit

12009999
Pkg of 1, includes Amplification mix, Primer-probe mix and positive control

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Accessories

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DG8 Cartridges QX200

17005222
Pkg of 24, cartridges for use with the QX200 Droplet Generator

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$286.00 
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DG8 Gaskets for QX200 Droplet Generator

17005223
Pkg of 24, gaskets for use with QX200 Droplet Generator

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$71.00 
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DG8 Cartridge Holder

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Pkg of 1, cartridge holder, positions and holds the cartridge in the QX200 Droplet Generator

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$699.00 
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ddPCR Droplet Reader Oil

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2 L (2 x 1 L), oil for use with droplet reader in the QX200 Droplet Reader

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$1,556.00 
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Pierceable Foil Heat Seals

17005225
Pkg of 100, foil seals for PCR and QX200 Droplet Digital PCR System applications, for use with PX1 PCR Plate Sealer

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$101.00 
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ddPCR Plates 96-Well, Semi-Skirted

17005224
Pkg of 25, clear well/clear shell semi-skirted plates, for use with QX200

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$170.00 
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Buffered Peptone Water Plus

3554179
Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions

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$28.00 
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Buffered Peptone Water Plus

3564684
Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions

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$78.00 
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Selective STEC Supplement

3564005
Selective STEC Supplement, vial 1 g (235 tests)

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$355.00 
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Equipment

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QX200 Droplet Generator

17005227
Droplet generator for digital PCR applications

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QX200 Droplet Reader

17005228
Droplet reader for digital PCR applications

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Laptop for QX200

17006702
Laptop for QX200 Droplet Digital PCR System

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$2,340.00 
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PX1 PCR Plate Sealer

17005226
PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord

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$4,305.00 
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CFX96 Touch Deep Well Real-Time PCR Detection System

3600037
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.

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Documents

Number Description Options
7210 Droplet Digital PCR for Food Testing brochure Click to download
10000105757 dd-Check STEC kit user guide Click to download
10000110358 QX200 Droplet Reader and Qx IDE Manager Software user guide Click to download
7186 dd-Check STEC Solution Quick Guide Click to download
7249 Droplet Digital PCR - Publications for Food Applications Click to download

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