Overview
The dd-Check STEC Solution uses Droplet Digital PCR technology to detect double-positive linked virulence genes (stx and eae) in a single bacterium from samples containing bacteria with single-positive or unlinked virulence genes, reducing the number of false positive Shiga toxin-producing Escherichia coli (STEC) results. This detection and linkage verification of targets in a single bacterium enhances the accuracy of pathogenic E. coli testing. Bio-Rad's dd-Check STEC Solution Receives AOAC International Approval.
Features and Benefits
- Less false positive results saves time and money with less unnecessary confirmations
- Confidence in results
- Decreasing time to results of screening and confirmation allows product to go to market faster
- Comply with regulations that classify STEC as an adulterant in food and keep up with changes in regulations
Applications and Uses
- Test for the detection and colocalization of virulence genes in Shiga toxin-producing Escherichia coli (STEC)
Data Analysis
The Qx IDE Manager Software — the optimal software for data analysis — was designed specifically for food safety applications. It provides complete automated analysis for the dd-Check STEC Assay. Results interpretation is based on the presence or absence of the two targets (stx and eae) and on the linkage analysis showing the colocalization of these virulence genes coming from a single bacterium.
dd-Check STEC Workflow
The dd-Check STEC Workflow is scalable to allow users the flexibility to test 1 to 94 samples per plate. Laboratories can use the dd-Check STEC Solution as an initial screening tool or after screening with the iQ-Check STEC Kits for next-day confirmation of pathogenic E. coli.
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Accessories
Pkg of 1, cartridge holder, positions and holds the cartridge in the QX200 Droplet Generator
Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions
Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions
Selective STEC Supplement, vial 1 g (235 tests)
Equipment
96-well, thermal cycler for PCR with cables
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.