dd-Check STEC Solution

dd-Check STEC Solution

dd-Check STEC kit provides a way to detect and confirm linked virulence genes and allows descrimination between true positive and true negative EHEC samples.

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Overview

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The dd-Check STEC Solution uses Droplet Digital PCR technology to detect double-positive linked virulence genes (stx and eae) in a single bacterium from samples containing bacteria with single-positive or unlinked virulence genes, reducing the number of false positive Shiga toxin-producing Escherichia coli (STEC) results. This detection and linkage verification of targets in a single bacterium enhances the accuracy of pathogenic E. coli testing. Bio-Rad's dd-Check STEC Solution Receives AOAC International Approval.

Features and Benefits

  • Less false positive results saves time and money with less unnecessary confirmations
  • Confidence in results
  • Decreasing time to results of screening and confirmation allows product to go to market faster
  • Comply with regulations that classify STEC as an adulterant in food and keep up with changes in regulations

Applications and Uses

  • Test for the detection and colocalization of virulence genes in Shiga toxin-producing Escherichia coli (STEC)

Data Analysis

The Qx IDE Manager Software — the optimal software for data analysis — was designed specifically for food safety applications. It provides complete automated analysis for the dd-Check STEC Assay. Results interpretation is based on the presence or absence of the two targets (stx and eae) and on the linkage analysis showing the colocalization of these virulence genes coming from a single bacterium.

dd-Check STEC Workflow

The dd-Check STEC Workflow is scalable to allow users the flexibility to test 1 to 94 samples per plate. Laboratories can use the dd-Check STEC Solution as an initial screening tool or after screening with the iQ-Check STEC Kits for next-day confirmation of pathogenic E. coli.

dd-check stec workflow

 

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Accessories

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(Discontinued) Pierceable Foil Heat Seals
17005225
Pkg of 100, foil seals for PCR and QX200 Droplet Digital PCR System applications, for use with PX1 PCR Plate Sealer

Equipment

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(Discontinued) PX1 PCR Plate Sealer
17005226
PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord
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(Discontinued) CFX96 Touch Deep Well Real-Time PCR Detection System
3600037

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.

Documents

Number Description Options
7210
Droplet Digital PCR for Food Testing brochure
10000105757
dd-Check STEC kit user guide
10000110358
QX200 Droplet Reader and Qx IDE Manager Software user guide
7186
dd-Check STEC Solution Quick Guide
7249
Droplet Digital PCR - Publications for Food Applications
3423
dd-Check STEC Solution, product information sheet
AOAC-ddPCR-poster
Scientific poster: Comparison Between Droplet Digital PCR and qPCR for Screening and Confirming Shiga Toxin-Producing E. coli (STEC) with Linked and Unlinked stx and eae in Beef Matrices
USDA-ddPCR-poster
Scientific poster: Detection of Enterohemorrhagic Escherichia coli in Beef Products Using Droplet Digital PCR