CFT Ceramic Fluoroapatite Support

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Fluoroapatite Resin

Overview

Flow rate on IgG Capture
Upper panel, binding capacity of CFT Type I (orange) and Type II (blue). Lower panel, effect of flow rate on IgG capture using CFT Type II. Bovine IgG (1 mg/ml) was run on an 11 x 47.6 mm column packed with 4 g CFT until an A280 of 0.80 was reached. The loading buffer was 10 mM sodium phosphate, pH 6.75. Elution was performed using a step gradient from 10 to 400 mM sodium phosphate. The binding capacities were 37.0, 31.2, and 31.0 mg/ml, respectively, at 150, 300, and 450 cm/hr. Chromatography was performed on the BioLogic DuoFlow system.
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CFT ceramic fluoroapatite (Ca10(PO4)6F2) is a rigid, spherical macroporous support used in the purification of biologically active compounds. CFT is a composite of fluoroapatite and hydroxyapatite prepared by chemically converting hydroxyapatite nanocrystals to fluoroapatite with a fluorine reagent.

CFT can be used under stringent chromatography conditions to separate acidic proteins requiring buffered conditions as low as pH 5, with minimal effect on the solubility or lifetime of the support.

CFT has high binding capacity and may be used reproducibly over an extended number of chromatography runs. Its increased tensile strength, chemical durability, and density provide excellent throughput and consistent performance for all separations, including biopharmaceutical process-scale manufacturing.

Features of CFT include:

  • Acidic protein separation for applications requiring pH as low as 5.0
  • High-density particles for fast, simple column packing
  • Sintering at 700°C (Type II) for heavy-duty, durable supports
  • Rigid particles for fast cleaning and equilibration
  • Inorganic calcium phosphate for distinct selectivities

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO9001 registered corporation.

Specifications and Characteristics  
Functional groups Ca2+, PO43-, F-
Nominal mean particle size 40 ± 4 µm
Nominal pore diameter range 600–800 Â
Recommended linear flow rate range 50–300 cm/hr
Operating pH range 5–14
Chemical compatibility (>24 hr) 2 N NaOH, 8 M urea, 6 M guanidine HCl
Regeneration 400 mM sodium phosphate, pH 6.8
Sanitation 1–2 N NaOH or KOH
Autoclavibility (121°C, 20 min) Yes
Packing density (g/ml packed bed) 0.86 g/ml
Dynamic binding capacity 14–21.5 mg lysozyme/g
Maximum operating pressure 55 bar (800 psi)
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CFT Ceramic Fluoroapatite

1585200
10 g, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size

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CFT Ceramic Fluoroapatite

1575000
100 g, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size

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CFT Ceramic Fluoroapatite

157-5100
1 kg, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size

6 1575100 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/157-5100_0921120655582.xml 1575100 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jal7kco8 2017-11-14 07:08:03 true true internet/product_SKU jal7kco8 templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/157-5100_0921120655582.xml LSR 2019-05-04 20:55:46 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555998739157 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:55:46 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/157-5100_0921120655582.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/157-5100_0921120655582.xml 1575100-cft-ceramic-fluoroapatite 1575100 CFT Ceramic Fluoroapatite CFT Ceramic Fluoroapatite 1 kg, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size 1 kg, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size 1 kg, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size /webroot/web/images/lsr/products/chromatography/sku_thumb/global/157-5100_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/157-5100_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/157-5100_feature.jpg RepEx 157-5100 1 kg, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Media N 0 false null false $ null false false true true false USD true false true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1575100&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1575100&CartQuote=QT&quantity= 1335 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1575100&AddToDefaultWishList=true&quantity= 23151800 null 157-5100
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CFT Ceramic Fluoroapatite

157-5500
5 kg, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size

7 1575500 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/157-5500_0921120655603.xml 1575500 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jal7kcnm 2017-11-14 07:08:03 true true internet/product_SKU jal7kcnm templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/157-5500_0921120655603.xml LSR 2019-05-04 20:56:06 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555998739199 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:56:06 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/157-5500_0921120655603.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/157-5500_0921120655603.xml 1575500-cft-ceramic-fluoroapatite 1575500 CFT Ceramic Fluoroapatite CFT Ceramic Fluoroapatite 5 kg, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size 5 kg, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size 5 kg, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size /webroot/web/images/ps/products/multimodal_media/sku_thumb/global/157-5500_tn.jpg /webroot/web/images/ps/products/multimodal_media/sku_view/global/157-5500_view.jpg /webroot/web/images/ps/products/multimodal_media/sku_feature/global/157-5500_feature.jpg RepEx 157-5500 5 kg, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Media N 0 false null false $ null false false true true false USD true false true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1575500&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1575500&CartQuote=QT&quantity= 1335 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1575500&AddToDefaultWishList=true&quantity= 12000000 null 157-5500
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Number Description Options
4110183 Instruction Manual, CFT Ceramic Fluoroapatite, Rev A Click to download
3111 CFT Ceramic Fluoroapatite Product Information Sheet, Rev B Click to download
6982 Mixed-Mode Resins/Media Selection Card Click to download [ Add to Cart (Free) ]
6789 IgG Polyclonal Antibody Purification Resin Selection Card, Ver A Click to download
6791 Virus Purification Resin Selection Card, Ver A Click to download
6870 Ceramic Apatite-Based Chromatography Media Publications List, Ver A Click to download
5853 Chimeric IgG Monoclonal Antibody Purification: A Comparative Study Using CHT Ceramic Hydroxyapatite and CFT Ceramic Fluoroapatite Chromatographic Media, Rev A Click to download

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