CFT Ceramic Fluoroapatite Support

Overview

Flow rate on IgG Capture

Upper panel, binding capacity of CFT Type I (orange) and Type II (blue). Lower panel, effect of flow rate on IgG capture using CFT Type II. Bovine IgG (1 mg/ml) was run on an 11 x 47.6 mm column packed with 4 g CFT until an A280 of 0.80 was reached. The loading buffer was 10 mM sodium phosphate, pH 6.75. Elution was performed using a step gradient from 10 to 400 mM sodium phosphate. The binding capacities were 37.0, 31.2, and 31.0 mg/ml, respectively, at 150, 300, and 450 cm/hr. Chromatography was performed on the BioLogic DuoFlow system.

CFT ceramic fluoroapatite is a rigid, spherical macroporous support used in the purification of biologically active compounds. CFT is a composite of fluoroapatite and hydroxyapatite.
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Description
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Description

CFT ceramic fluoroapatite (Ca₁₀(PO₄)₆F₂) is a rigid, spherical macroporous support used in the purification of biologically active compounds. CFT is a composite of fluoroapatite and hydroxyapatite prepared by chemically converting hydroxyapatite nanocrystals to fluoroapatite with a fluorine reagent.

CFT can be used under stringent chromatography conditions to separate acidic proteins requiring buffered conditions as low as pH 5, with minimal effect on the solubility or lifetime of the support.

CFT has high binding capacity and may be used reproducibly over an extended number of chromatography runs. Its increased tensile strength, chemical durability, and density provide excellent throughput and consistent performance for all separations, including biopharmaceutical process-scale manufacturing.

Features of CFT include:

Acidic protein separation for applications requiring pH as low as 5.0
High-density particles for fast, simple column packing
Sintering at 700°C (Type II) for heavy-duty, durable supports
Rigid particles for fast cleaning and equilibration
Inorganic calcium phosphate for distinct selectivities

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO9001 registered corporation.

Specifications

Specifications and Characteristics
Functional groups	Ca²⁺, PO₄^3-, F^-
Nominal mean particle size	40 ± 4 µm
Nominal pore diameter range	600–800 Â
Recommended linear flow rate range	50–300 cm/hr
Operating pH range	5–14
Chemical compatibility (>24 hr)	2 N NaOH, 8 M urea, 6 M guanidine HCl
Regeneration	400 mM sodium phosphate, pH 6.8
Sanitation	1–2 N NaOH or KOH
Autoclavibility (121°C, 20 min)	Yes
Packing density (g/ml packed bed)	0.86 g/ml
Dynamic binding capacity	14–21.5 mg lysozyme/g
Maximum operating pressure	55 bar (800 psi)

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CFT Ceramic Fluoroapatite

1585200
10 g, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size

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CFT Ceramic Fluoroapatite

1575000
100 g, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size

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CFT Ceramic Fluoroapatite

157-5100
1 kg, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size

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CFT Ceramic Fluoroapatite

157-5500
5 kg, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size

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Documents

Number	Description	Options
4110183	Instruction Manual, CFT Ceramic Fluoroapatite	Click to download
3111	CFT Ceramic Fluoroapatite Product Information Sheet, Rev B	Click to download
6982	Mixed-Mode Resins/Media Selection Guide	Click to download	6982 [ Add to Cart (Free) ] 0
6789	IgG Polyclonal Antibody Purification Resin Selection Card, Ver A	Click to download
6791	Virus Purification Resin Selection Card, Ver A	Click to download
6870	Ceramic Apatite-Based Chromatography Media Publications List, Ver A	Click to download
5853	Chimeric IgG Monoclonal Antibody Purification: A Comparative Study Using CHT Ceramic Hydroxyapatite and CFT Ceramic Fluoroapatite Chromatographic Media, Rev A	Click to download

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CFT Ceramic Fluoroapatite Support

Overview

Description

Specifications

Ordering

CFT Ceramic Fluoroapatite

CFT Ceramic Fluoroapatite

CFT Ceramic Fluoroapatite

CFT Ceramic Fluoroapatite

Documents

I cannot find the Certificate of Analysis I am looking for. Help!

Where can I find the catalog number, SKU number, or product number?

Where can I find the lot or control number?

Why are Certificates of Analysis not on the Documents tab?

I have a batch number, not a lot/control number. How do I find the Certificate of Analysis for my item?

Can I get a Certificate of Analysis even if my item is expired?

Why does it say "Product Name Not Found" for a Certificate of Analysis listed in the search results?

Why does it say "Not Available" for a Certificate of Analysis listed in the search results?

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