CFT Ceramic Fluoroapatite Support

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Overview

CFT ceramic fluoroapatite is a rigid, spherical macroporous support used in the purification of biologically active compounds. CFT is a composite of fluoroapatite and hydroxyapatite.
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Description

Description

 

CFT ceramic fluoroapatite (Ca10(PO4)6F2) is a rigid, spherical macroporous support used in the purification of biologically active compounds. CFT is a composite of fluoroapatite and hydroxyapatite prepared by chemically converting hydroxyapatite nanocrystals to fluoroapatite with a fluorine reagent.

CFT can be used under stringent chromatography conditions to separate acidic proteins requiring buffered conditions as low as pH 5, with minimal effect on the solubility or lifetime of the support.

CFT has high binding capacity and may be used reproducibly over an extended number of chromatography runs. Its increased tensile strength, chemical durability, and density provide excellent throughput and consistent performance for all separations, including biopharmaceutical process-scale manufacturing.

Features of CFT include:

  • Acidic protein separation for applications requiring pH as low as 5.0
  • High-density particles for fast, simple column packing
  • Sintering at 700°C (Type II) for heavy-duty, durable supports
  • Rigid particles for fast cleaning and equilibration
  • Inorganic calcium phosphate for distinct selectivities

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO9001 registered corporation.

Specifications

Specifications

Specifications and Characteristics
 
Functional groups
Ca2+, PO43-, F-
Nominal mean particle size
40 ± 4 µm
Nominal pore diameter range
600–800 Â
Recommended linear flow rate range
50–300 cm/hr
Operating pH range
5–14
Chemical compatibility (>24 hr)
2 N NaOH, 8 M urea, 6 M guanidine HCl
Regeneration
400 mM sodium phosphate, pH 6.8
Sanitation
1–2 N NaOH or KOH
Autoclavibility (121°C, 20 min)
Yes
Packing density (g/ml packed bed)
0.86 g/ml
Dynamic binding capacity
14–21.5 mg lysozyme/g
Maximum operating pressure
55 bar (800 psi)

Ordering

Ordering

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1585200
10 g, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size
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1575000
100 g, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size
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157-5100
1 kg, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size
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157-5500
5 kg, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size

Documents

Documents

Number Description Options
4110183
Instruction Manual, CFT Ceramic Fluoroapatite
3111
CFT Ceramic Fluoroapatite Product Information Sheet, Rev B
6982
Mixed-Mode Resins/Media Selection Guide
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6789
IgG Polyclonal Antibody Purification Resin Selection Card, Ver A
6791
Virus Purification Resin Selection Card, Ver A
6870
Ceramic Apatite-Based Chromatography Media Publications List, Ver A
5853
Chimeric IgG Monoclonal Antibody Purification: A Comparative Study Using CHT Ceramic Hydroxyapatite and CFT Ceramic Fluoroapatite Chromatographic Media, Rev A