Bio-Plex® Precision Pro™ Human Cytokine Assays

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Bio-Plex Precision Pro Human Cytokine Assays

Overview

Working Assay Range*
Analyte Serum Plasma
IL-1beta 0.2–516 0.2–556
IL-2 2.7–4,569 4.5–4,570
IL-4 0.3–2,611 0.2–2,611
IL-5 3.7–4,354 5.0–4,355
IL-6 1.7–16,058 5.2–18,618
IL-10 0.7–8,591 1.1–11,850
IL-12 (p70) 0.6–3,993 1.7–3,994
IL-13 0.2–1,745 0.3–2,406
IFN-gamma 0.5–1,814 0.7–1,814
TNF-alpha 0.2–1,423 0.2–2,059

* Results (in pg/ml) based on an average of 5 assays meeting specifications for precision and accuracy. For both serum and plasma samples, intra-assay CV was ≤8% and inter-assay CV was ≤10%.

Bio-Plex® Technology and Applications
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The Bio-Plex Precision Pro™ (magnetic) human cytokine immunoassay panel contains highly sensitive, highly reproducible, magnetic bead–based assays which allow accurate measurement of low levels of human cytokines. These assays feature both low intra– and inter-assay %CVs, resulting in highly precise measurements. This panel detects the following targets:

  • IL-1β
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-10
  • IL-12 (p70)
  • IL-13
  • IFN-γ
  • TNF-α

Based on 8 µm magnetic beads, these assays are compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

Key Features

  • Magnetic beads for simplified plate processing
  • Contains controls for generating QC Samples
  • Includes standard diluents for both serum and plasma
  • All-in-one, space-saving complete kit
  • Premixed standards for generation of curves over a broad dynamic range for each target

Instrument Compatibility

  • Bio-Plex 200 system
  • Bio-Plex Pro™ wash station
  • Bio-Plex Pro II wash station

This product is not compatible with the Bio-Plex® MAGPIX™ and Bio-Plex 3D systems due to the bead size.

More Information

See how Bio-Plex instruments and assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

Limit of detection, pg/ml ≤1
Precision  
Intra-assay CV <10%
Inter-assay CV <15%
Accuracy (% recovery) 80–120%
Cross-reactivity ≤1%
Dynamic range, pg/ml at high PMT 1–2,500
Matrices Plasma, serum, culture supernatant, lysates, homogenates, other biological fluids
Data vary by assay.  
Immunoassay for Cytokines
Bead type Magnetic
Species Human
Number Description Options
5405 Bio-Plex® Multiplex System Brochure, Ver E Click to download [ Add to Cart (Free) ]
5629 Bio-Plex™ Precision Pro Human Cytokine Assay Panel Product Sheet, Rev B Click to download
10008318 Bio-Plex Precision Pro™ Cytokine Assay Instruction Manual, Rev A Click to download
BR125FS Second Generation Multiplex Immunoassays Click to download
6457 The Bio-Plex Success Guide for Multiplex Immunoassays, Rev B Click to download
6458 Bio-Plex Success Guide/Assay Sample Prep, Rev B Click to download

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

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