Droplet Digital PCR (ddPCR) Tools for Cell Therapy

Cell therapy has rapidly gained popularity in the immunotherapy world for treating cancer, but ensuring safety and effectiveness in patients is complex. Research and clinical laboratories around the world are utilizing Bio-Rad's ddPCR technology as a tool to overcome challenges with existing methods for a range of applications including CAR transgene quantification.

CAR-T Cell Monitoring by Droplet Digital PCR

CAR-T Axi-Cel ddPCR Assay

A recent publication (Fehse, 2020) described the development of a ddPCR assay for precise quantification of CD19-CAR-T cells down to 0.01% frequency from patients treated with Axicabtagene ciloleucel (Axi-cel)*.

Bio-Rad worked with Dr. Boris Fehse to make this CAR-T Axi-Cel assay available.

  • Detect Axi-Cel CD19-CAR-T DNA
  • Duplex with a reference copy number HEX assay such as RPP30
  • Optimized for QX ONE and QX200 Droplet Digital PCR Systems
  • For Research Use Only—Not for use in diagnostic procedures

How to order the CAR-T Axi-Cel ddPCR assay

  • Order 1

    Click the button below or go to
    Bio-Rad.com/Axi-Cel-ddPCR

  • Order 2

    Log in to your Bio-Rad.com account; if you do not have an account, choose Register Today

  • Order 3

    Select Add to My Designs

  • Order 4

    Click Add Assay to Cart

  • Order 5

    Add to cart any reagents and consumables needed and check out

Buy CAR-T Axi-Cel ddPCR Assay

Other reagents required:

 
 

CAR-T Webinar

Dr. Fehse discusses how Droplet Digital PCR detects gene-modified cells in different body fluids

In-vivo monitoring of CAR-T cells using sensitive Droplet Digital PCR

Dr. Boris Fehse, Head of Research Cell and Gene Therapy University Clinical Hospital, Hamburg

Prof. Fehse relates how his team uses ddPCR to quantify Axi-cel and Tisa-cel CAR-T cell expansion and persistence after treatment. The ddPCR assay achieves highly precise and sensitive quantification without the resistance to PCR inhibition often seen with qPCR.

View Webinar

New WORKFLOW Count Modified Cells with the ddPCR Whole Cell DNA Workflow

Quantify transgenes or edits at single cell resolution

  • Accurately measure the % of modified cells at the DNA level
  • Robust sensitive detection enables low cell input requirement (100–1000 cells)
  • Scalable solution with easy workflow and analysis
Buy Enhancer Reagent for ddPCR Whole Cell DNA Workflow
 

ddPCR Whole Cell DNA Workflow

  • One

    Add cells to 1x ddPCR reaction

    Cells added to ddPCR reaction
  • Two

    Encapsulate whole cells in droplets

    Whole cell encapsulation in droplets with the QX200 Droplet Generator
  • Three

    Perform end-point PCR

    Perform end-point PCR with the C1000 Touch Thermal Cycler
  • Four

    Read and analyze results

    Analyze droplets with the QX200 Droplet Reader

Droplet Digital PCR Systems

Bio-Rad's Droplet Digital PCR Systems provide ultrasensitive and absolute nucleic acid quantification. Droplet Digital PCR is particularly useful for low-abundance targets, targets in complex backgrounds, or monitoring subtle changes in target levels that cannot be detected with real-time PCR.

QX ONE Droplet Digital PCR System

QX ONE Droplet Digital PCR System

  • Walk-away operation with integrated ddPCR workflow and 5 plate capacity
  • Advanced multiplexing capability 4 four color channels
  • Easy to use using large onboard touchscreen with simple run setup
  • Regulatory compliance enabled by QX ONE Software, Regulatory Edition
View QX ONE System
 
QX200 AutoDG Droplet Digital PCR System

QX200 AutoDG Droplet Digital PCR System

  • Simplifies ddPCR workflow and ensures consistency
  • Generates droplets for 96 ddPCR reactions in 45 minutes
  • One AutoDG Instrument can supply 4–5 droplet readers
  • HEPA-filtered enclosure reduces contamination risk
QX200 AutoDG Droplet Digital PCR System

QX200 Droplet Digital PCR System

  • Simple and easy-to-use workflow with 96-sample throughput
  • Droplet partitioning reduces bias from amplification efficiency and PCR inhibitors
  • Convenient assay design — standard curves are not required