Image

Description
Description
The ddPCR Whole Cell DNA Workflow, comprised of ddPCR Enhancer Reagent A and standard ddPCR materials, enables researchers to accurately count modified cells that have their gene(s) of interest, edit(s) or knockout.
ddPCR Whole Cell DNA Assay Workflow
Features and Benefits
Key Features | Benefits |
---|---|
Single-cell DNA readout |
|
Reproducible |
|
Easy |
|
Sensitivity |
|
Product Contents
- 1 ml vial of ddPCR Enhancer Reagent A
- Product insert card (10000128504)
- Certificate of Analysis
Packaging Option
Catalog No. | Number of 20 µl Reactions | Size |
---|---|---|
12013425 | 1,000 | 1 vial |
Additional Required Materials
The following additional reagents, equipment, and consumables are needed to perform the ddPCR Whole Cell DNA Assay:
Required Reagents
Catalog No. | Description |
---|---|
12013425 | ddPCR Enhancer Reagent A, 1000 reactions |
12005910 | ddPCR Multiplex Supermix, 1000 reactions |
dHsaCP2500313 | RPP30 FAM Copy Number Assay, 1000 reactions |
dHsaCNS642774914 | SOD1 HEX Copy Number Assay, 1000 reactions |
NEB catalog #R0108S or #R0527S |
Restriction enzymes HaeIII or Brsl |
Required Equipment and Consumables
Catalog No. | Description |
---|---|
1864002 | QX200 Droplet Generator |
1864001 | QX200 Droplet Digital PCR System (includes QX200 Droplet Generator) |
1814000 | PX1 PCR Plate Sealer |
1851197 | C1000 Touch Thermal Cycler with 96–Deep Well Reaction Module |
12001925 | ddPCR 96-Well Plates, 25 plates |
1863005 | Droplet Generation Oil, 10 x 7 ml |
1864007 | DG8 Cartridges for QX200 Droplet Generator, 5 pkg of 24 |
1814040 | PCR Plate Heat Seal, pierceable foil, 100 seals |
1863004 | ddPCR Droplet Reader Oil, 2 L |
Supporting Documents
- ddPCR Whole Cell DNA Workflow Protocol