Droplet Digital PCR (ddPCR) Enhancer Reagent A #12013425

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12013425 [x-forwarded-proto] = [https] [accept-language] = [en-US,en;q=0.5] [x-forwarded-port] = [443] [x-forwarded-for] = [3.226.248.180, 10.232.3.119] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [seourl] = [/en-us/sku/12013425-droplet-digital-pcr-ddpcr-enhancer-reagent] [x-amzn-trace-id] = [Root=1-5f730d33-0d9889442e7ce3e866b3e361] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=12013425] [host] = [10.232.16.28:1776] [x-request-uri] = [/en-us/sku/12013425-droplet-digital-pcr-ddpcr-enhancer-reagent] [connection] = [Keep-Alive] [accept-encoding] = [br,gzip] [user-agent] = [CCBot/2.0 (https://commoncrawl.org/faq/)] Catalog/ProductSKU pageStyleKey 12013425-droplet-digital-pcr-ddpcr-enhancer-reagent LSR en US en-us /prd false 07/30/13 03:25 AM en_US true internet/generic 2020-09-18 04:55:37 /default/main/bio-rad/STAGING 1375179935592 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml page_EDUPriceOverlay /default/main/bio-rad/WORKAREA/default jddgmm0t 2017-11-14 06:29:46 true jddgmm0t templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml LSE N /default/main/bio-rad en 07/30/37 05:00 PM 1579275613146 2020-09-18 04:55:37 /default/main/bio-rad/WORKAREA/default/templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml edu-price-overlay 1 1375179935592 page_EDUPriceOverlay <div id="eduPriceOverlay" class="overlay" style="display: block;"> <p class="headerClassText">What is the education discount policy?</p> <p class="descClassText">For more than 20 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows K&ndash;12 and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices. These discounted products are indicated by an EDU suffix (for example, 1660003EDU).</p> <p class="headerClassText">How do I apply for the education discount?</p> <p class="descClassText">If you are an educator at the K&ndash;12 or college level, contact your local <a href="/en-au/contact-us">Customer Service</a> office to establish an education account number.</p> </div> 0 /webroot/web/html EDU_price_overlay.html /webroot/web/html/EDU_price_overlay.html sandeep Edu Edu Price Overlay Edu Price Overlay Content 07/30/13 03:25 AM 07/30/37 05:00 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE N 0 {"ecommerce":{"detail":{"products":{"price":"$1,000.00","name":"Droplet Digital PCR (ddPCR) Enhancer Reagent A","variant":"BRC","id":"12013425","category":"BRC","brand":"BRC"}}},"event":"detail"} 12013425 /default/main/bio-rad/WORKAREA/default 05/28/20 05:31 PM en_US k9m07znd 2020-05-28 17:35:07 true true internet/product_SKU k9m07znd templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12013425_1590687307.xml LSR 2020-07-28 18:48:16 /default/main/bio-rad/STAGING N /default/main/bio-rad en 05/28/37 07:34 AM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,VN,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE 2020-07-28 18:48:16 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12013425_1590687307.xml templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12013425_1590687307.xml 12013425-droplet-digital-pcr-ddpcr-enhancer-reagent 1 12013425 ddPCR Enhancer Reagent A Droplet Digital PCR (ddPCR) Enhancer Reagent A 1 ml vial of ddPCR Enhancer Reagent A 1 ml vial of ddPCR Enhancer Reagent A /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/12013425_th.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/12013425_view.jpg preview TW 12013425 1 ml vial of ddPCR Enhancer Reagent A sc-ddPCR, ddPCR whole cell DNA assay, single-cell ddPCR, enhancer reagent, ddPCR enhancer reagent, ddPCR whole cell DNA workflow 05/28/20 05:31 PM 05/28/37 07:34 AM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,VN,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE LSR /LSR/Amplification---PCR/Droplet-Digital-PCR-System N 0 <div> <p>The ddPCR Whole Cell DNA Workflow, comprised of ddPCR Enhancer Reagent A and standard ddPCR materials, enables researchers to accurately count modified cells that have their gene(s) of interest, edit(s) or knockout.</p> <div class="corner-box top-margin bg-white80"> <h3 class="opt-subhead30">ddPCR Whole Cell DNA Assay Workflow</h3> <div class="slider-class"> <ul class="slider mid-align step-wf ddpcr-cell-counting-wf"> <li> <div><img src="/webroot/web/images/lsr/products/amplification_pcr/category_feature/global/ddpcr-cell-counting-wf-1.png" alt="One" /></div> <div> <h3 class="opt-subhead">Add cells* to 1x ddPCR reaction</h3> </div> <div><img src="/webroot/web/images/lsr/products/amplification_pcr/category_feature/global/ddpcr-cell-counting-wf-1-ddpcr-add-cells.png" alt="Cells added to ddPCR reaction" /></div> <p class="caption">* Add 1&ndash;60,000 cells. No cell preparation is required (cells in phosphate buffered saline with 1% bovine serum albumin).</p> </li> <li> <div><img src="/webroot/web/images/lsr/products/amplification_pcr/category_feature/global/ddpcr-cell-counting-wf-2.png" alt="Two" /></div> <div> <h3 class="opt-subhead">Encapsulate whole cells in droplets</h3> </div> <div><img src="/webroot/web/images/lsr/products/amplification_pcr/category_feature/global/ddpcr-cell-counting-wf-2-ddpcr-encapsulate.png" alt="Whole cell encapsulation in droplets with the QX200 Droplet Generator" style="width: 300px;" /></div> </li> <li> <div><img src="/webroot/web/images/lsr/products/amplification_pcr/category_feature/global/ddpcr-cell-counting-wf-3.png" alt="Three" /></div> <div> <h3 class="opt-subhead">Perform end-point PCR</h3> </div> <div><img src="/webroot/web/images/lsr/products/amplification_pcr/category_feature/global/ddpcr-cell-counting-wf-3-ddpcr-end-point.png" alt="Perform end-point PCR with the C1000 Touch Thermal Cycler" /></div> </li> <li> <div><img src="/webroot/web/images/lsr/products/amplification_pcr/category_feature/global/ddpcr-cell-counting-wf-4.png" alt="Four" /></div> <div> <h3 class="opt-subhead">Read and analyze results</h3> </div> <div><img src="/webroot/web/images/lsr/products/amplification_pcr/category_feature/global/ddpcr-cell-counting-wf-4-ddpcr-analyze.png" alt="Analyze droplets with the QX200 Droplet Reader" style="width: 300px;" /></div> </li> </ul> </div> </div> <h2 class="descHeader">Features and Benefits</h2> <table class="pd_table pd_gridlines-alternate"> <thead> <tr style="height: 16px;"> <th width="20%" scope="col" style="text-align: left; height: 16px;"><strong>Key Features</strong></th> <th width="65%" scope="col" style="text-align: left; height: 16px;"><strong>Benefits</strong></th> </tr> </thead> <tbody> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">Single-cell DNA readout</td> <td style="height: 16px;"> <ul> <li>Can measure modified cells with edits(s), knockouts</li> <li>Can measure cells modified to remove receptor (allogeneic product)</li> </ul> </td> </tr> <tr style="height: 16px;"> <td scope="row" valign="top" style="height: 16px;">Reproducible</td> <td> <ul> <li>Increased efficiency, reduce cost, time to results</li> </ul> </td> </tr> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">Easy</td> <td style="height: 16px;"> <ul> <li>Faster analysis, faster onboarding, technician operated</li> </ul> </td> </tr> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">Sensitivity</td> <td style="height: 16px;"> <ul> <li>Enable to detect low transfection efficiency</li> </ul> </td> </tr> </tbody> </table> <h2 class="descHeader">Product Contents</h2> <ul> <li>1 ml vial of ddPCR Enhancer Reagent A</li> <li>Product insert card (10000128504)</li> <li>Certificate of Analysis</li> </ul> <h2 class="descHeader">Packaging Option</h2> <table class="pd_table pd_gridlines-alternate"> <thead> <tr> <th scope="col" align="left">Catalog No.</th> <th scope="col" style="text-align: left;">Number of 20 &micro;l Reactions</th> <th scope="col" style="text-align: left;">Size</th> </tr> </thead> <tbody> <tr> <td scope="row">17005726</td> <td align="center" style="text-align: left;">1,000</td> <td align="center" style="text-align: left;">1 vial</td> </tr> </tbody> </table> <br /> <div class="breakoutfeature bg-edf6e4"> <h2>Additional Required Materials</h2> <p>The following additional reagents, equipment, and consumables are needed to perform the ddPCR Whole Cell DNA Assay:</p> <h3 class="descHeader">Required Reagents</h3> <table class="pd_table pd_gridlines-alternate"> <thead> <tr style="height: 16px;"> <th width="20%" scope="col" style="text-align: left; height: 16px;"><strong>Catalog No.</strong></th> <th width="65%" scope="col" style="text-align: left; height: 16px;"><strong>Description</strong></th> </tr> </thead> <tbody> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">12013425</td> <td style="height: 16px;">ddPCR Enhancer Reagent A, 1000 reactions</td> </tr> <tr style="height: 16px;"> <td scope="row" valign="top" style="height: 16px;">12005910</td> <td>ddPCR Multiplex Supermix, 1000 reactions</td> </tr> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">dHsaCP2500313</td> <td style="height: 16px;">RPP30 FAM Copy Number Assay, 1000 reactions</td> </tr> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">dHsaCNS642774914</td> <td style="height: 16px;">SOD1 HEX Copy Number Assay, 1000 reactions</td> </tr> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">NEB catalog #R0108S<br />or #R0527S</td> <td style="height: 16px;">Restriction enzymes HaeIII or Brsl</td> </tr> </tbody> </table> <br /> <h3 class="descHeader">Required Equipment and Consumables</h3> <table class="pd_table pd_gridlines-alternate"> <thead> <tr style="height: 16px;"> <th width="20%" scope="col" style="text-align: left; height: 16px;"><strong>Catalog No.</strong></th> <th width="65%" scope="col" style="text-align: left; height: 16px;"><strong>Description</strong></th> </tr> </thead> <tbody> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">1864002</td> <td style="height: 16px;">QX200 Droplet Generator</td> </tr> <tr style="height: 16px;"> <td scope="row" valign="top" style="height: 16px;">1864001</td> <td>QX200 Droplet Digital PCR System<br />(includes QX200 Droplet Generator)</td> </tr> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">1814000</td> <td style="height: 16px;">PX1 PCR Plate Sealer</td> </tr> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">1851197</td> <td style="height: 16px;">C1000 Touch Thermal Cycler with 96&ndash;Deep Well Reaction Module</td> </tr> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">12001925</td> <td style="height: 16px;">ddPCR 96-Well Plates, 25 plates</td> </tr> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">1863005</td> <td style="height: 16px;">Droplet Generation Oil, 10 x 7 ml</td> </tr> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">1864007</td> <td style="height: 16px;">DG8 Cartridges for QX200 Droplet Generator, 5 pkg of 24</td> </tr> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">1814040</td> <td style="height: 16px;">PCR Plate Heat Seal, pierceable foil, 100 seals</td> </tr> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">1863004</td> <td style="height: 16px;">ddPCR Droplet Reader Oil, 2 L</td> </tr> </tbody> </table> </div> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>ddPCR Whole Cell DNA Workflow&nbsp;<a href="/webroot/web/pdf/lsr/literature/Bulletin_7321.pdf" target="_blank" rel="noopener noreferrer">Protocol</a></li> </ul> <script type="text/javascript" src="/webroot/web/js/jquery.bxslider-min.js"></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function () { $('head').append('<link rel="stylesheet" href="/webroot/web/css/bxslider-min.css" type="text/css" media="screen" />'); 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1 ml vial of ddPCR Enhancer Reagent A

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No cell preparation is required (cells in phosphate buffered saline with 1% bovine serum albumin).</p> </li> <li> <div><img src="/webroot/web/images/lsr/products/amplification_pcr/category_feature/global/ddpcr-cell-counting-wf-2.png" alt="Two" /></div> <div> <h3 class="opt-subhead">Encapsulate whole cells in droplets</h3> </div> <div><img src="/webroot/web/images/lsr/products/amplification_pcr/category_feature/global/ddpcr-cell-counting-wf-2-ddpcr-encapsulate.png" alt="Whole cell encapsulation in droplets with the QX200 Droplet Generator" style="width: 300px;" /></div> </li> <li> <div><img src="/webroot/web/images/lsr/products/amplification_pcr/category_feature/global/ddpcr-cell-counting-wf-3.png" alt="Three" /></div> <div> <h3 class="opt-subhead">Perform end-point PCR</h3> </div> <div><img src="/webroot/web/images/lsr/products/amplification_pcr/category_feature/global/ddpcr-cell-counting-wf-3-ddpcr-end-point.png" alt="Perform end-point PCR with the C1000 Touch Thermal Cycler" /></div> </li> <li> <div><img src="/webroot/web/images/lsr/products/amplification_pcr/category_feature/global/ddpcr-cell-counting-wf-4.png" alt="Four" /></div> <div> <h3 class="opt-subhead">Read and analyze results</h3> </div> <div><img src="/webroot/web/images/lsr/products/amplification_pcr/category_feature/global/ddpcr-cell-counting-wf-4-ddpcr-analyze.png" alt="Analyze droplets with the QX200 Droplet Reader" style="width: 300px;" /></div> </li> </ul> </div> </div> <h2 class="descHeader">Features and Benefits</h2> <table class="pd_table pd_gridlines-alternate"> <thead> <tr style="height: 16px;"> <th width="20%" scope="col" style="text-align: left; height: 16px;"><strong>Key Features</strong></th> <th width="65%" scope="col" style="text-align: left; height: 16px;"><strong>Benefits</strong></th> </tr> </thead> <tbody> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">Single-cell DNA readout</td> <td style="height: 16px;"> <ul> <li>Can measure modified cells with edits(s), knockouts</li> <li>Can measure cells modified to remove receptor (allogeneic product)</li> </ul> </td> </tr> <tr style="height: 16px;"> <td scope="row" valign="top" style="height: 16px;">Reproducible</td> <td> <ul> <li>Increased efficiency, reduce cost, time to results</li> </ul> </td> </tr> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">Easy</td> <td style="height: 16px;"> <ul> <li>Faster analysis, faster onboarding, technician operated</li> </ul> </td> </tr> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">Sensitivity</td> <td style="height: 16px;"> <ul> <li>Enable to detect low transfection efficiency</li> </ul> </td> </tr> </tbody> </table> <h2 class="descHeader">Product Contents</h2> <ul> <li>1 ml vial of ddPCR Enhancer Reagent A</li> <li>Product insert card (10000128504)</li> <li>Certificate of Analysis</li> </ul> <h2 class="descHeader">Packaging Option</h2> <table class="pd_table pd_gridlines-alternate"> <thead> <tr> <th scope="col" align="left">Catalog No.</th> <th scope="col" style="text-align: left;">Number of 20 &micro;l Reactions</th> <th scope="col" style="text-align: left;">Size</th> </tr> </thead> <tbody> <tr> <td scope="row">17005726</td> <td align="center" style="text-align: left;">1,000</td> <td align="center" style="text-align: left;">1 vial</td> </tr> </tbody> </table> <br /> <div class="breakoutfeature bg-edf6e4"> <h2>Additional Required Materials</h2> <p>The following additional reagents, equipment, and consumables are needed to perform the ddPCR Whole Cell DNA Assay:</p> <h3 class="descHeader">Required Reagents</h3> <table class="pd_table pd_gridlines-alternate"> <thead> <tr style="height: 16px;"> <th width="20%" scope="col" style="text-align: left; height: 16px;"><strong>Catalog No.</strong></th> <th width="65%" scope="col" style="text-align: left; height: 16px;"><strong>Description</strong></th> </tr> </thead> <tbody> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">12013425</td> <td style="height: 16px;">ddPCR Enhancer Reagent A, 1000 reactions</td> </tr> <tr style="height: 16px;"> <td scope="row" valign="top" style="height: 16px;">12005910</td> <td>ddPCR Multiplex Supermix, 1000 reactions</td> </tr> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">dHsaCP2500313</td> <td style="height: 16px;">RPP30 FAM Copy Number Assay, 1000 reactions</td> </tr> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">dHsaCNS642774914</td> <td style="height: 16px;">SOD1 HEX Copy Number Assay, 1000 reactions</td> </tr> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">NEB catalog #R0108S<br />or #R0527S</td> <td style="height: 16px;">Restriction enzymes HaeIII or Brsl</td> </tr> </tbody> </table> <br /> <h3 class="descHeader">Required Equipment and Consumables</h3> <table class="pd_table pd_gridlines-alternate"> <thead> <tr style="height: 16px;"> <th width="20%" scope="col" style="text-align: left; height: 16px;"><strong>Catalog No.</strong></th> <th width="65%" scope="col" style="text-align: left; height: 16px;"><strong>Description</strong></th> </tr> </thead> <tbody> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">1864002</td> <td style="height: 16px;">QX200 Droplet Generator</td> </tr> <tr style="height: 16px;"> <td scope="row" valign="top" style="height: 16px;">1864001</td> <td>QX200 Droplet Digital PCR System<br />(includes QX200 Droplet Generator)</td> </tr> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">1814000</td> <td style="height: 16px;">PX1 PCR Plate Sealer</td> </tr> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">1851197</td> <td style="height: 16px;">C1000 Touch Thermal Cycler with 96&ndash;Deep Well Reaction Module</td> </tr> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">12001925</td> <td style="height: 16px;">ddPCR 96-Well Plates, 25 plates</td> </tr> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">1863005</td> <td style="height: 16px;">Droplet Generation Oil, 10 x 7 ml</td> </tr> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">1864007</td> <td style="height: 16px;">DG8 Cartridges for QX200 Droplet Generator, 5 pkg of 24</td> </tr> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">1814040</td> <td style="height: 16px;">PCR Plate Heat Seal, pierceable foil, 100 seals</td> </tr> <tr style="height: 16px;"> <td scope="row" style="height: 16px;">1863004</td> <td style="height: 16px;">ddPCR Droplet Reader Oil, 2 L</td> </tr> </tbody> </table> </div> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>ddPCR Whole Cell DNA Workflow&nbsp;<a href="/webroot/web/pdf/lsr/literature/Bulletin_7321.pdf" target="_blank" rel="noopener noreferrer">Protocol</a></li> </ul> <script type="text/javascript" src="/webroot/web/js/jquery.bxslider-min.js"></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function () { $('head').append('<link rel="stylesheet" href="/webroot/web/css/bxslider-min.css" type="text/css" media="screen" />'); 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return false; }); }); }); // ]]></script> </div> 1 0 false $1,000.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12013425&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12013425&CartQuote=QT&quantity= 1357 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12013425&AddToDefaultWishList=true&quantity= 12000000 1000.00 SKU detail page 120-13425-MSDS ddPCR Enhancer Reagent A Australia English /webroot/web/pdf/WWMSDS/LSGC/AUS/AUS_ENG_12013425.pdf Korea Korean /webroot/web/pdf/WWMSDS/LSGC/ROK/ROK_KOR_12013425.pdf Belgium French /webroot/web/pdf/WWMSDS/LSGC/B/B_FR_12013425.pdf Italy Spanish /webroot/web/pdf/WWMSDS/LSGC/ISL/ISL_ES_12013425.pdf Mexico Spanish /webroot/web/pdf/WWMSDS/LSGC/MEX/MEX_ES_12013425.pdf Canada French /webroot/web/pdf/WWMSDS/LSGC/FCN/FCN_FR_12013425.pdf The Netherlands Dutch /webroot/web/pdf/WWMSDS/LSGC/NL/NL_NL_12013425.pdf Russian Federation Russian /webroot/web/pdf/WWMSDS/LSGC/RUS/RUS_RUS_12013425.pdf Hungary English /webroot/web/pdf/WWMSDS/LSGC/HE/HE_ENG_12013425.pdf Hungary Hungarian /webroot/web/pdf/WWMSDS/LSGC/H/H_UNG_12013425.pdf Brazil Portuguese /webroot/web/pdf/WWMSDS/LSGC/BRA/BRA_PT_12013425.pdf Germany German /webroot/web/pdf/WWMSDS/LSGC/D/D_D_12013425.pdf Czech Republic Czech /webroot/web/pdf/WWMSDS/LSGC/CZ/CZ_TS_12013425.pdf Denmark Danish /webroot/web/pdf/WWMSDS/LSGC/DK/DK_DA_12013425.pdf Denmark English /webroot/web/pdf/WWMSDS/LSGC/DKE/DKE_ENG_12013425.pdf Turkey Turkish /webroot/web/pdf/WWMSDS/LSGC/TR/TR_TR_12013425.pdf Germany English /webroot/web/pdf/WWMSDS/LSGC/DE/DE_ENG_12013425.pdf Poland Polish /webroot/web/pdf/WWMSDS/LSGC/PL/PL_POL_12013425.pdf Belgium English /webroot/web/pdf/WWMSDS/LSGC/BE/BE_ENG_12013425.pdf Hong Kong English /webroot/web/pdf/WWMSDS/LSGC/HK/HK_ENG_12013425.pdf Italy Italian /webroot/web/pdf/WWMSDS/LSGC/I/I_IT_12013425.pdf Portugal Portuguese /webroot/web/pdf/WWMSDS/LSGC/P/P_PT_12013425.pdf Ireland English /webroot/web/pdf/WWMSDS/LSGC/IE/IE_ENG_12013425.pdf Canada English /webroot/web/pdf/WWMSDS/LSGC/CDN/CDN_USA_12013425.pdf USA English /webroot/web/pdf/WWMSDS/LSGC/USA/USA_USA_12013425.pdf true China Chinese /webroot/web/pdf/WWMSDS/LSGC/RC/RC_ZH_12013425.pdf Spain Spanish /webroot/web/pdf/WWMSDS/LSGC/E/E_ES_12013425.pdf Sweden Swedish /webroot/web/pdf/WWMSDS/LSGC/S/S_S_12013425.pdf Singapore English /webroot/web/pdf/WWMSDS/LSGC/SGP/SGP_ENG_12013425.pdf Czech Republic English /webroot/web/pdf/WWMSDS/LSGC/CZE/CZE_ENG_12013425.pdf United Kingdom English /webroot/web/pdf/WWMSDS/LSGC/GB/GB_ENG_12013425.pdf Austria English /webroot/web/pdf/WWMSDS/LSGC/AE/AE_ENG_12013425.pdf Sweden English /webroot/web/pdf/WWMSDS/LSGC/SE/SE_ENG_12013425.pdf France English /webroot/web/pdf/WWMSDS/LSGC/FE/FE_ENG_12013425.pdf New Zealand English /webroot/web/pdf/WWMSDS/LSGC/NZ/NZ_ENG_12013425.pdf Switzerland German /webroot/web/pdf/WWMSDS/LSGC/CH/CH_D_12013425.pdf China English /webroot/web/pdf/WWMSDS/LSGC/PRC/PRC_ENG_12013425.pdf India English /webroot/web/pdf/WWMSDS/LSGC/IN/IN_ENG_12013425.pdf Switzerland English /webroot/web/pdf/WWMSDS/LSGC/CHE/CHE_ENG_12013425.pdf Finland Finnish /webroot/web/pdf/WWMSDS/LSGC/SF/SF_SF_12013425.pdf Israel English /webroot/web/pdf/WWMSDS/LSGC/IL/IL_ENG_12013425.pdf Poland English /webroot/web/pdf/WWMSDS/LSGC/POL/POL_ENG_12013425.pdf France French /webroot/web/pdf/WWMSDS/LSGC/F/F_FR_12013425.pdf The Netherlands English /webroot/web/pdf/WWMSDS/LSGC/NLE/NLE_ENG_12013425.pdf Austria German /webroot/web/pdf/WWMSDS/LSGC/A/A_D_12013425.pdf Switzerland French /webroot/web/pdf/WWMSDS/LSGC/CHF/CHF_FR_12013425.pdf Norway Norwegian /webroot/web/pdf/WWMSDS/LSGC/N/N_N_12013425.pdf
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The ddPCR Whole Cell DNA Workflow, comprised of ddPCR Enhancer Reagent A and standard ddPCR materials, enables researchers to accurately count modified cells that have their gene(s) of interest, edit(s) or knockout.

ddPCR Whole Cell DNA Assay Workflow

  • One

    Add cells* to 1x ddPCR reaction

    Cells added to ddPCR reaction

    * Add 1–60,000 cells. No cell preparation is required (cells in phosphate buffered saline with 1% bovine serum albumin).

  • Two

    Encapsulate whole cells in droplets

    Whole cell encapsulation in droplets with the QX200 Droplet Generator
  • Three

    Perform end-point PCR

    Perform end-point PCR with the C1000 Touch Thermal Cycler
  • Four

    Read and analyze results

    Analyze droplets with the QX200 Droplet Reader

Features and Benefits

Key Features Benefits
Single-cell DNA readout
  • Can measure modified cells with edits(s), knockouts
  • Can measure cells modified to remove receptor (allogeneic product)
Reproducible
  • Increased efficiency, reduce cost, time to results
Easy
  • Faster analysis, faster onboarding, technician operated
Sensitivity
  • Enable to detect low transfection efficiency

Product Contents

  • 1 ml vial of ddPCR Enhancer Reagent A
  • Product insert card (10000128504)
  • Certificate of Analysis

Packaging Option

Catalog No. Number of 20 µl Reactions Size
17005726 1,000 1 vial

Additional Required Materials

The following additional reagents, equipment, and consumables are needed to perform the ddPCR Whole Cell DNA Assay:

Required Reagents

Catalog No. Description
12013425 ddPCR Enhancer Reagent A, 1000 reactions
12005910 ddPCR Multiplex Supermix, 1000 reactions
dHsaCP2500313 RPP30 FAM Copy Number Assay, 1000 reactions
dHsaCNS642774914 SOD1 HEX Copy Number Assay, 1000 reactions
NEB catalog #R0108S
or #R0527S
Restriction enzymes HaeIII or Brsl

Required Equipment and Consumables

Catalog No. Description
1864002 QX200 Droplet Generator
1864001 QX200 Droplet Digital PCR System
(includes QX200 Droplet Generator)
1814000 PX1 PCR Plate Sealer
1851197 C1000 Touch Thermal Cycler with 96–Deep Well Reaction Module
12001925 ddPCR 96-Well Plates, 25 plates
1863005 Droplet Generation Oil, 10 x 7 ml
1864007 DG8 Cartridges for QX200 Droplet Generator, 5 pkg of 24
1814040 PCR Plate Heat Seal, pierceable foil, 100 seals
1863004 ddPCR Droplet Reader Oil, 2 L

Supporting Documents

  • ddPCR Whole Cell DNA Workflow Protocol
Number Description Options
Available Translation
ddPCR Enhancer Reagent A  (SDS)gene

Bulletin# 120-13425-MSDS

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