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      Droplet Digital PCR (ddPCR) Enhancer Reagent A #12013425

      12013425_view.jpg

      Overview

      1 ml vial of ddPCR Enhancer Reagent A
      Safety Data Sheet

      Safety Data Sheet

      Description

      Description

      The ddPCR Whole Cell DNA Workflow, comprised of ddPCR Enhancer Reagent A and standard ddPCR materials, enables researchers to accurately count modified cells that have their gene(s) of interest, edit(s) or knockout.

      ddPCR Whole Cell DNA Assay Workflow

      • One

        Add cells* to 1x ddPCR reaction

        Cells added to ddPCR reaction

        * Add 1–60,000 cells. No cell preparation is required (cells in phosphate buffered saline with 1% bovine serum albumin).

      • Two

        Encapsulate whole cells in droplets

        Whole cell encapsulation in droplets with the QX200 Droplet Generator
      • Three

        Perform end-point PCR

        Perform end-point PCR with the C1000 Touch Thermal Cycler
      • Four

        Read and analyze results

        Analyze droplets with the QX200 Droplet Reader

      Features and Benefits

      Key Features Benefits
      Single-cell DNA readout
      • Can measure modified cells with edits(s), knockouts
      • Can measure cells modified to remove receptor (allogeneic product)
      Reproducible
      • Increased efficiency, reduce cost, time to results
      Easy
      • Faster analysis, faster onboarding, technician operated
      Sensitivity
      • Enable to detect low transfection efficiency

      Product Contents

      • 1 ml vial of ddPCR Enhancer Reagent A
      • Product insert card (10000128504)
      • Certificate of Analysis

      Packaging Option

      Catalog No. Number of 20 µl Reactions Size
      12013425 1,000 1 vial

      Additional Required Materials

      The following additional reagents, equipment, and consumables are needed to perform the ddPCR Whole Cell DNA Assay:

      Required Reagents

      Catalog No. Description
      12013425 ddPCR Enhancer Reagent A, 1000 reactions
      12005910 ddPCR Multiplex Supermix, 1000 reactions
      dHsaCP2500313 RPP30 FAM Copy Number Assay, 1000 reactions
      dHsaCNS642774914 SOD1 HEX Copy Number Assay, 1000 reactions
      NEB catalog #R0108S
      or #R0527S      		 Restriction enzymes HaeIII or Brsl

      Required Equipment and Consumables

      Catalog No. Description
      1864002 QX200 Droplet Generator
      1864001 QX200 Droplet Digital PCR System
      (includes QX200 Droplet Generator)
      1814000 PX1 PCR Plate Sealer
      1851197 C1000 Touch Thermal Cycler with 96–Deep Well Reaction Module
      12001925 ddPCR 96-Well Plates, 25 plates
      1863005 Droplet Generation Oil, 10 x 7 ml
      1864007 DG8 Cartridges for QX200 Droplet Generator, 5 pkg of 24
      1814040 PCR Plate Heat Seal, pierceable foil, 100 seals
      1863004 ddPCR Droplet Reader Oil, 2 L

      Supporting Documents

      • ddPCR Whole Cell DNA Workflow Protocol