BioPlex 2200 Multiplex Testing

BioPlex^® 2200 Overview

A Fully-Automated, Random Access, Multiplex Platform

The BioPlex 2200 System is the industry's first and only fully-automated, random access, multiplex testing platform. Combined with a multi-marker, disease-oriented assay menu, internal quality control technologies, and eFlex™ Software, the BioPlex 2200 represents a breakthrough in clinical diagnostic technology. This groundbreaking system offers significant laboratory cost savings through consolidation, decreased labor, and reduced reagent consumption.

Multiplex Testing with the BioPlex^® 2200 System

Patented Multiplex Detection Magnetic Bead Technology

BioPlex^® 2200 immunoassays use heterogeneous sets of magnetic 8 µm beads. The beads are infused with varying ratios of fluorescent dyes, creating unique bead sets. Beads within each set are coated with a ligand (i.e., antigen, antibody, analyte, etc.) specific to a particular assay. Bead sets are then mixed together in a single reagent pack allowing for simultaneous detection of multiple analytes from a single sample.

Each microsphere is dyed with a unique combination of 2 colors.

Up to 25 color-coded microspheres (bead regions), can be bound with a unique antigen

Example: ANA Screen Kit

The ANA Screen Reagent Pack uses 16 microspheres: 13 assay beads and 3 quality control checks.

	13 Assay Microspheres 1 dsDNA 2 Chromatin 3 Ribosomal P 4 SS-A (52 kD)* 5 SS-A (60 kD)* 6 SS-B 7 Sm 8 Sm/RNP 9 RNP (A)* 10 RNP (68)* 11 Scl-70 12 Jo-1 13 Centromere B * Individual SS-A and RNP results not available in the U.S. Reported as SS-A and RNP for a total of 11 individual results.
3 Quality Control Microspheres A Serum Verification Bead (SVB) B Internal Standard Bead (ISB) C Reagent Blank Bead (RBB)