BioPlex 2200 Multiplex Testing

BioPlex 2200 Overview

A Fully-Automated, Random Access, Multiplex Platform

The BioPlex 2200 System is the industry's first and only fully-automated, random access, multiplex testing platform. Combined with a multi-marker, disease-oriented assay menu, internal quality control technologies, and eFlex Software, the BioPlex 2200 represents a breakthrough in clinical diagnostic technology. This groundbreaking system offers significant laboratory cost savings through consolidation, decreased labor, and reduced reagent consumption.

Multiplex Testing with the BioPlex 2200 System

Patented Multiplex Detection Magnetic Bead Technology

BioPlex 2200 immunoassays use heterogeneous sets of magnetic 8 µm beads. The beads are infused with varying ratios of fluorescent dyes, creating unique bead sets. Beads within each set are coated with a ligand (i.e., antigen, antibody, analyte, etc.) specific to a particular assay. Bead sets are then mixed together in a single reagent pack allowing for simultaneous detection of multiple analytes from a single sample.

Each microsphere is dyed with a unique combination of 2 colors.

Up to 25 color-coded microspheres (bead regions), can be bound with a unique antigen

Example: ANA Screen Kit

The ANA Screen Reagent Pack uses 16 microspheres: 13 assay beads and 3 quality control checks.

	13 Assay Microspheres 1 dsDNA 2 Chromatin 3 Ribosomal P 4 SS-A (52 kD)* 5 SS-A (60 kD)* 6 SS-B 7 Sm 8 Sm/RNP 9 RNP (A)* 10 RNP (68)* 11 Scl-70 12 Jo-1 13 Centromere B * Individual SS-A and RNP results not available in the U.S. Reported as SS-A and RNP for a total of 11 individual results.
3 Quality Control Microspheres A Serum Verification Bead (SVB) B Internal Standard Bead (ISB) C Reagent Blank Bead (RBB)