How to Migrate Assays from Quantitative PCR to Droplet Digital™ PCR (ddPCR™)
Droplet Digital™ PCR (ddPCR™) provides consistent results day-to-day and lab-to-lab compared with qPCR. And, transitioning your assays from established qPCR to ddPCR technology is simple and straightforward. Get guidance on how to transition from qPCR to ddPCR technology and the differences and similarities to consider between the two methods based on AAV titering assay study.
What you will learn in this article:
- The experimental differences between qPCR and ddPCR technology
- How to account for variables and mitigate sources of error
- How to calculate a qPCR to ddPCR data conversion factor
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