Power Through PCR With Proven Thermal Cyclers
Get Consistent Results with Dependable, Precise Cyclers for Routine & High-Throughput PCR
Bio-Rad Thermal Cyclers
The new PTC Harmony Thermal Cycler expands our family of instruments by delivering trusted thermal performance and essential functionality in a cost‑effective design that doesn’t compromise on quality. For more demanding PCR workflows, the high‑end PTC Tempo Thermal Cycler offers advanced features and automation compatibility, while the compact T100 Thermal Cycler provides an ideal solution for routine PCR. All Bio‑Rad thermal cyclers feature Peltier technology for precise temperature control and a thermal gradient function for easy PCR assay optimization in a single run.
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| Overview | Premium performance on a modern platform. For demanding applications and multiple users. | High performance on a modern platform. | Robust and economical for routine PCR with a compact footprint. |
| System Tour | - | ||
| Block formats |
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| 96-well: 0.2 ml high-profile |
| Recommended Sample Volume Range |
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| 96-well: 15–100 µl |
| PCR consumables | Both high and low-profile tubes and plates | Both high and low-profile tubes and plates | Only high-profile tubes and plates |
| Real-time PCR compatibility | N/A | No | N/A |
| User interface | 8" graphical touch screen | 8" graphical touch screen | 5.7" graphical touch screen |
| Performance |
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| Size (W x D x H) | 28 x 50 x 26 cm (11 x 20 x 10 in) | 28 x 50 x 27 cm (11 x 20 x 11 in) | 26 x 47 x 23 cm (10 x 18 x 9 in.) |
| Weight | 96, Deepwell, 384: 15 kg (33 lb) 48/48: 14 kg (31 lb) | 96, Deepwell: 15 kg (33 lb) | 9 kg (20 lb) |
| Connectivity | BR.io, USB A 2.0, Ethernet, barcode scanner | USB A 2.0, Ethernet, barcode scanner | N/A |
| Memory capacity | <100,000 protocols; unlimited with a USB drive | <100,000 protocols; unlimited with a USB drive | <500 protocols; unlimited with a USB drive |
| Documentation | User Manual Specifications | User Manual Specifications | User Manual Specifications |
Reagents & Plastics Selectors
PCR & qPCR Reagents Selector
Find the best reagents for your PCR, qPCR, and RT-qPCR experiments using our interactive selection tool.
PCR Plastics Selector
Use the PCR Plastics Selector to find the best plates, tubes, seals, and accessories for your PCR and qPCR experiments and determine instrument compatibility.
Resources
Automation That Works Around Your Workflow
Fully automate PCR and qPCR to boost lab productivity and ensure data reliability. Your PTC Tempo and CFX Opus Systems integrate with the automation platform of your choice, giving you the flexibility to build an automated system that fits your lab.
Thermal Gradient for Easy Protocol Optimization
Learn about the advantages of thermal gradients on all of Bio-Rad's thermal cyclers and real-time PCR systems.

Importance of Quality PCR Plastics
Read about how quality PCR plastics indirectly influence sensitivity and reproducibility of your experiments.

Amplification Reagents and Plastics
(PDF 879 KB)Download this brochure for an overview of all our PCR reagent and plastics offerings, specifications, performance data, and more.

PCR Plastics Instrument Compatibility
(PDF 6.1 MB)Determine the compatibility of PCR consumables with thermal cycling and real-time detection PCR systems.
Applications & Technologies
What is PCR (Polymerase Chain Reaction)
Learn about the proper design, optimization, analysis, and troubleshooting of polymerase chain reactions, as well as required PCR instrumentation and reagents.
PCR Instrumentation
Guidelines for selecting the right thermal cycler for your experiments.
Introduction to PCR Analysis
Learn about the different methods used to analyze products of PCR reactions.
PCR Troubleshooting
Find possible causes and treatments for common PCR problems.
