ddPCR Mutation Detection Assays

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ddPCR Mutation detection assays can be found and purchased on Bio-Rad.com/digital-assays. A wet-lab validated mutation detection assay is returned if available, or an assay is designed instantly for your target. Wet-lab validated mutation detection assays detect mutant DNA present at 0.1% in a background of WT DNA in one well. An even better sensitivity of 0.005–0.1% can generally be achieved by using multiple reaction wells. Browse list of wet-lab validated Mutation Detection Assays.

Mutation detection assays can be designed instantly for over 4 million mutations, listed in the COSMIC database, on Bio-Rad’s Digital Assay Site. Built by the experts in digital PCR at Bio-Rad's Digital Biology Center, the Bio-Rad Digital Assay Site design engine takes into consideration everything from GC content and Tm, to primer placement and probe length, even genome specificity when designing an assay for you.

Key Benefits

• Wet-lab validated assays available for more than 200 targets
• Wet-lab validated assays guaranteed to work when used as instructed
• Validation data available for review and download
• Assays designed instantly for targets and sequences for which validated assays are not available
• Suitable for use with circulating tumor DNA, liquid biopsy, and FFPE and other tissue samples
• Orderable in single-tube or two-tube formats
• Positive control sequences provided

Sample Data

High sensitivity of PrimePCR ddPCR mutation detection assays provide precise quantification of JAK2 V617F mutation in the presence of wild-type DNA. A, 2-D fluorescence amplitude plot shows duplicate wells of a mixed mutant: wild-type sample across multiple percentage mutant blends. The black cluster on the plot represents the negative droplets, the green cluster represents the droplets that are positive for wild-type DNA only, the blue cluster represents the droplets that are positive for mutant DNA only, and the orange cluster represents the droplets that are positive for both wild-type and mutant targets. B, 2-D fluorescence amplitude plot shows duplicate wells of a wild-type–only sample. C, fractional abundance plot shows the percentage frequency (orange markers) of the mutant DNA in a wild-type DNA background. All error bars are generated by QuantaSoft software and represent the 95% confidence interval.

Packaging Options

ddPCR Mutation Detection Assays are available in single tube or two-tube formats.

Single-tube format

The single-tube formats of the ddPCR Mutation Detection Assays contain a set of primers, a mutant, and wild-type probe. The single tube format assays are 20X concentrated, ready-to-use-primer-probe mix optimized for use with ddPCR Supermix for Probes (no dUTP). Each assay contains 18 uM each primer and 5uM each probe. The single-tube formats provide added convenience as the mutant and WT probes are pre-mixed and allow for the addition of an extra sample in the ddPCR reaction.

Two-tube formats

The mutant and WT probes are provided separately.

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