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How ddPCR Technology Outperforms qPCR for Contamination Detection
While quantitative polymerase chain reaction (qPCR) is superior to other culture- and stain-based approaches, this relative measure cannot achieve accurate and sensitive detection of contamination, which is critical for avoiding costly late-stage mitigation strategies. Many therapeutic antibody developers are turning to Droplet Digital™ PCR (ddPCR™) technology, a rapid, sensitive, and specific measure that allows for absolute quantitation.
Read this article to learn:
- Why qPCR is not suitable for accurate detection at very low input DNA concentrations
- How ddPCR can provide more accurate contamination readouts through aboslute quantification and by differentiating free-floating DNA from that of live cells
- How you can immediately gain improvements in sensitivity and specificity of detection by transitioning from qPCR to ddPCR technology
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