Overview
Description
Description
Precision Melt Supermix is a superior high-resolution melt (HRM) supermix delivering optimal discrimination for numerous applications including:
- Genotyping
- Mutation analyses
- Methylation studies
- Species identification
- Epigenetic analyses
- De novo SNP discovery
Key Features, Benefits and Applications of Precision Melt Supermix
- Sensitive and specific discrimination of class I–IV single nucleotide polymorphisms (SNPs) across a broad range of amplicons
- Ideal solution for insertion/deletions >6 base pairs
- Accurate detection of the percent of CpG methylation status for epigenetic studies
- Ideal for mutation screening of small mutations or using a primer walking approach for larger regions
- Ideal cost-saving application for upstream and downstream next generation sequencing pre-screens and post-validations
- Exceptional room temperature stability for high-throughput HRM studies
- Optimized formulation containing EvaGreen dye delivers robust PCR and HRM performance
Precision Melt Analysis™ Software
Precision Melt Analysis Software imports and analyzes data files generated from all CFX Real-Time PCR Detection Systems to genotype samples based on the thermal denaturation properties of double-stranded DNA.
Instrument Compatibility
For use with all HRM-ready real-time PCR systems; dye calibration may be required.
Packaging Options
| Number of 20 µl Reactions | Volume | Catalog Number |
| 200 | 2 ml | 1725110 |
| 1,000 | 10 ml | 1725112 |
Related Products
- Precision Melt Analysis™ Software
- Melt calibration kit (184-5020)
Related Categories
- Real-Time PCR Supermixes
- Real-Time PCR Detection Systems
- PrimePCR™ PCR Primers & Assays
- PCR Plastic Consumables
Online Resources
Specifications
Specifications
Storage at –20°C
Guaranteed for 6 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)
Ordering
Ordering
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(Discontinued) Precision Melt Supermix for High Resolution Melt (HRM) Analysis, 200 x 20 µl reactions, 2 x 1 ml
1725110
2 x 1 ml, 2x real-time PCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, EvaGreen dye, stabilizers, for 200 x 20 µl reactions
Image
(Discontinued) Precision Melt Supermix for High Resolution Melt (HRM) Analysis, 1,000 x 20 µl reactions, 10 x 1 ml
1725112
10 x 1 ml, 2x real-time PCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, EvaGreen dye, stabilizers, for 1,000 x 20 µl reactions