Protein Blotting Detection and Imaging

Print

Обзор

Total protein detection and immunodetection can be performed using colorimetric, chemiluminescence, and fluorescence development and imaging techniques. Once proteins have been transferred to a membrane, they can be visualized using a variety of specialized detection reagents. Total protein detection allows visualization of all the proteins on the blot while immunological detection (immunodetection) methods employ antibody or ligand conjugates for visualization of specific proteins of interest. This section reviews the various total protein and immunological detection methods available.

Related Topics: Protein Blotting Methods, Membranes and Blotting Papers, Transfer Buffers, and Transfer Conditions.


See how you can streamline your protein electrophoresis and western blotting experiments.

Find out more »
Stain-Free Technology
 
 
Page Contents
 
 
 

Protein Detection Systems

Protein Detection Systems

Protein detection systems and their sensitivity.

 

Похожее содержимое

 
Literature
Number Description Download
2895 Protein Blotting Guide, Ver C Click to download
2032 Western Blotting Detection Reagents Brochure, Rev F Click to download
Number Description Options
6216 Detection Buffer Formulations Click to download
6217 Total Protein Detection Click to download
6218 Blot Stripping & Reprobing Click to download
6219 Immunodetection Click to download
 
 
Похожие технологии
LUSPULKSY [x-forwarded-proto] = [https] [accept-language] = [en-US,en;q=0.5] [x-forwarded-port] = [443] [x-forwarded-for] = [18.210.22.132, 10.232.2.87] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [seourl] = [/ru-ru/applications-technologies/protein-blotting-detection-imaging] [x-amzn-trace-id] = [Root=1-5dac70e4-b723d5ae54c215d6474f8828] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=LUSPULKSY] [host] = [10.232.1.21:1776] [x-request-uri] = [/ru-ru/applications-technologies/protein-blotting-detection-imaging] [connection] = [Keep-Alive] [accept-encoding] = [gzip] [user-agent] = [CCBot/2.0 (https://commoncrawl.org/faq/)] AppTech/AppTechDetails pageStyleKey internet/solutions_sub applications-technologies/protein-blotting-detection-imaging LSR LUSPULKSY Protein Blotting Detection and Imaging Protein Blotting Detection and Imaging /webroot/web/html/lsr/solutions/technologies/western_blotting <p>Total protein detection and immunodetection can be performed using colorimetric, chemiluminescence, and fluorescence development and imaging techniques. Once proteins have been transferred to a membrane, they can be visualized using a variety of specialized detection reagents. Total protein detection allows visualization of all the proteins on the blot while immunological detection (<a href="/evportal/destination/solutions?catID=LUSQ4HE8Z">immunodetection</a>) methods employ antibody or ligand conjugates for visualization of specific proteins of interest. This section reviews the various total protein and immunological detection methods available.</p> <p><strong>Related Topics</strong>: <a href="/evportal/destination/solutions?catID=LUSPPSESH">Protein Blotting Methods</a>, <a href="/evportal/destination/solutions?catID=LUSPSJ8UU">Membranes and Blotting Papers</a>, <a href="/evportal/destination/solutions?catID=LUSQA88UU">Transfer Buffers</a>, and <a href="/evportal/destination/solutions?catID=LUSPTIMNI">Transfer Conditions</a>.</p> <h3 class="clear"><br /></h3> <div class="bannerAT"> <div class="bannerText ddpcrText" style="width: 390px !important;"> <h2 class="banner_header">Stain-Free Technology</h2> <p style="padding:0 !important;margin:10px 0 !important;">See how you can streamline your protein electrophoresis and western blotting experiments.</p> <a class="linkgeneration" href="/en-us/applications-technologies/stain-free-technology">Find out more &raquo;</a></div> <img src="/webroot/web/images/lsr/global/english/solutions/stain-free-technology.jpg" alt="Stain-Free Technology" width="615" height="120" /></div> <script src="/webroot/web/js/countrySpecific-min.js" type="text/javascript"></script> <script type="text/javascript"><!-- $(document).ready(function(){ setSterlingUrlsToHtmlHrefVariables(); $('a.linkgeneration').each(function(){ $(this).attr('href', $(this).attr('href').replace('_locale', languageCode + '-' + countryCode).replace('_verticalUrl', currentVerticalUrlTitle).replace('_defaultVerticalUrl', defaultVerticalUrlTitle).replace('_feedbackCMSID',feedbackCMSID)); }); }); // --></script> Protein Detection Systems <p><img src="/webroot/web/images/lsr/solutions/technologies/protein_electrophoresis_blotting_and_imaging/western_blotting/technology_detail/pet25_protein_detection_systems.gif" alt="Protein Detection Systems" width="530px" height="580px" /></p> <p class="caption"><strong>Protein detection systems and their sensitivity.</strong></p> <div class="top"><a href="#helptop">Back to Top</a></div> Protocols <table id="carttablealigned" class="literature_table" style="height: auto; width: 583px;" border="0" cellspacing="0" cellpadding="0"> <tbody> <tr> <th>Number</th> <th>Description</th> <th class="options">Options</th> </tr> <tr> <td width="100">6216</td> <td width="350">Detection Buffer Formulations</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6216.pdf" target="_blank"><span>Click to download</span></a></td> </tr> <tr> <td width="100">6217</td> <td width="350">Total Protein Detection</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6217.pdf" target="_blank"><span>Click to download</span></a></td> </tr> <tr> <td width="100">6218</td> <td width="350">Blot Stripping &amp; Reprobing</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6218.pdf" target="_blank"><span>Click to download</span></a></td> </tr> <tr> <td width="100">6219</td> <td width="350">Immunodetection</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6219.pdf" target="_blank"><span>Click to download</span></a></td> </tr> </tbody> </table> 2895 2032 Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Western Blotting/Semi-Dry Blotting Systems/Trans-Blot Turbo Transfer System ->MTS::LGOQBW15##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Western Blotting/Semi-Dry Blotting Systems/Trans-Blot SD Semi-Dry Transfer Cell ->MT::b92801aa-76b7-45c9-825e-672589d01b00##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Western Blotting/Wet/Tank Blotting Systems/Mini Trans-Blot Cell ->MT::589ca8f7-5751-487a-a453-571ee8cc8b7e##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Western Blotting/Wet/Tank Blotting Systems/Criterion Blotter ->MT::351faa8e-680e-4892-9778-c8c722e9b3b2##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Western Blotting/Wet/Tank Blotting Systems/Trans-Blot Cell ->MT::a9900af6-f596-45c8-859e-9985048a418e## Life Science Research/Solutions/Technologies/2-D Electrophoresis ->MTS::LUSQG6LPT##Life Science Research/Solutions/Technologies/Protein Electrophoresis ->MTS::LUSOVO47B## Life Science Research/Solutions/Applications/Protein Separation and Analysis/Fast Separation Transfer and Analysis ->MTS::LUSOUMFCN## Eddie C Protein Blotting Detection and Imaging 12/08/11 11:12 AM 12/08/21 11:24 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Technologies/Western_Blotting N 0
Подпишитесь на рассылки от компании Bio-Rad здесь!
Введите свой адрес электронной почты ниже
LUSPULKSY [x-forwarded-proto] = [https] [accept-language] = [en-US,en;q=0.5] [x-forwarded-port] = [443] [x-forwarded-for] = [18.210.22.132, 10.232.2.87] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [seourl] = [/ru-ru/applications-technologies/protein-blotting-detection-imaging] [x-amzn-trace-id] = [Root=1-5dac70e4-b723d5ae54c215d6474f8828] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=LUSPULKSY] [host] = [10.232.1.21:1776] [x-request-uri] = [/ru-ru/applications-technologies/protein-blotting-detection-imaging] [connection] = [Keep-Alive] [accept-encoding] = [gzip] [user-agent] = [CCBot/2.0 (https://commoncrawl.org/faq/)] AppTech/AppTechDetails pageStyleKey applications-technologies/protein-blotting-detection-imaging LSR https://www.bio-rad-antibodies.com https://www.bio-rad.com 09/29/15 11:44 AM en_US true internet/generic 2019-07-01 01:54:38 /default/main/bio-rad/STAGING 1443552242541 RU templatedata/internet/generic/data/ru_RU/GNL/page_generic_footer_b2c_1443552305_RU.xml page_generic_footer_b2c /default/main/bio-rad/WORKAREA/default jcihubo9 2017-10-27 13:14:24 true Needs translation jcihubo9 templatedata/internet/generic/data/ru_RU/GNL/page_generic_footer_b2c_1443552305_RU.xml GNL N /default/main/bio-rad ru_RU 05/20/37 01:07 PM 1561767883929 2019-07-01 01:54:38 /default/main/bio-rad/WORKAREA/default/templatedata/internet/generic/data/ru_RU/GNL/page_generic_footer_b2c_1443552305_RU.xml footer 1 1443552242541 page_generic_footer_b2c <div class="col"> <h4>Support</h4> <ul> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/life-science-research/support?ID=1140">Life Science Support</a></li> <li><a class="wtclass linkgeneration" href="_acdDomain/technical-support.html">Antibodies Support</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/life-science-research/purchase-service-programs/expert-care-service?ID=1383780912356">Expert Care Service</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/contact">Contact Us</a></li> </ul> </div> <div class="col"> <h4>Documents</h4> <ul> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/literature-library">Literature Library</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/life-science-research/support/certificate-of-analysis">Certificate of Analysis</a></li> <li><a class="wtclass" href="https://myeinserts.qcnet.com/" target="_blank" rel="noopener noreferrer">QC Inserts</a></li> <li><a class="wtclass linkgeneration" href="_acdDomain/iso-9001-quality-assurance-management.html">Quality Certification</a></li> </ul> </div> <div class="col"> <h4>Ordering</h4> <ul> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/my-bio-rad">Quick Order</a></li> <li><a id="footer_orderLink" class="orderhistoryLink wtclass" href="javascript:void(0);">Order History</a></li> <li><a class="wtclass" id="footer_quoteLink" href="javascript:void(0);">Quote History</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/my-bio-rad">My Bio-Rad</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/life-science-research/purchase-service-programs/supply-center-program?ID=1383773882551">Supply Centers</a></li> </ul> </div> <div class="col"> <h4>Our Products</h4> <ul> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/life-science-research?ID=1100">Life Science Research</a></li> <li><a class="wtclass linkgeneration" href="_acdDomain/">Antibodies</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/clinical-diagnostics?ID=1200">Clinical Diagnostics</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/spectroscopy?ID=1300">Spectroscopy</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/process-separations?ID=1400">Process Separations</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/food-science?ID=1475">Food Science</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/education?ID=1450">Life Science Education</a></li> </ul> </div> <div class="col"> <h4>Company</h4> <ul> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/corporate/about-bio-rad?ID=1003">About Bio-Rad</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/corporate/careers?ID=1004">Careers</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/corporate/investor-relations?ID=1007">Investor Relations</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/corporate/newsroom?ID=1006">Newsroom</a></li> <li><a class="wtclass linkgeneration" href="_acdDomain/blog/">Blog</a></li> <li><a class="wtclass" target="_blank" href="https://www.bioradiations.com" rel="noopener noreferrer">Bioradiations</a></li> <li> <div class="social"> <ul> <li><a class="no-target-style brconly wtclass" href="https://www.linkedin.com/company/bio-rad?trk=top_nav_home" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/linkedin_round_40x40.png" alt="Bio-Rad LinkedIn" width="20" height="20" /></a> <a class="no-target-style bracd wtclass" style="display: none;" href="https://www.linkedin.com/company/bio-rad-antibodies" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/linkedin_round_40x40.png" alt="Bio-Rad Antibodies LinkedIn" width="20" height="20" /></a></li> <li><a class="no-target-style brconly wtclass" href="https://www.youtube.com/user/BioRadLifeScience" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/youtube-icon-rnd-40x40.png" alt="Bio-Rad YouTube" width="20" height="20" /></a> <a class="no-target-style bracd wtclass" style="display: none;" href="https://www.youtube.com/playlist?list=PLrAEgIY86I6w1jajKp3O9RQJEvJZ2ux0I" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/youtube-icon-rnd-40x40.png" alt="Bio-Rad Antibodies YouTube" width="20" height="20" /></a></li> <li><a class="no-target-style brconly wtclass" href="https://twitter.com/BioRadLifeSci" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/twitter_round_40x40.png" alt="Bio-Rad Twitter" width="20" height="20" /></a> <a class="no-target-style bracd wtclass" style="display: none;" href="https://twitter.com/BioRadAbs" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/twitter_round_40x40.png" alt="Bio-Rad Antibodies Twitter" width="20" height="20" /></a></li> <li><a class="no-target-style brconly wtclass" href="https://www.facebook.com/biorad/" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/facebook-icon-rnd-40x40.png" alt="Bio-Rad Facebook" width="20" height="20" /></a> <a class="no-target-style bracd wtclass" style="display: none;" href="https://www.facebook.com/BioRadAntibodies" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/facebook-icon-rnd-40x40.png" alt="Bio-Rad Antibodies Facebook" width="20" height="20" /></a></li> <li><a class="no-target-style wtclass" href="https://www.instagram.com/bioradlabs/" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/instagram-icon-rnd-40x40.png" alt="Bio-Rad Instagram" width="20" height="20" /></a></li> <li><a class="no-target-style wtclass" href="https://uk.pinterest.com/BioRadAbs/" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/pinterest40x40.png" alt="Bio-Rad Pinterest" width="20" height="20" /></a></li> </ul> </div> </li> </ul> </div> /webroot/web/html footer_page_b2c_en_RU.html /webroot/web/html/footer_page_b2c_en_RU.html SM Footer 09/29/15 11:44 AM 05/20/37 01:07 PM RU ru GNL /ru_RU/GNL N 0 ru-ru Главная страница Товарные знаки <script type="text/javascript">$(document).ready(function () {$('#emailLink').remove();});</script>Условия использования сайта Условия торговли Отпечатки Программа утилизации для ЕС Конфиденциальность Change Cookie Setting <script type="text/javascript">$(document).ready(function () {$(".links2").append('<style>.social-links-br {float: left;margin-right: 10px;}.social-links-br img {width: 20px;height: 20px;margin-right: 5px}</style><div class="social-links-br"><a class="no-target-style" href="https://www.linkedin.com/company/bio-rad?trk=top_nav_home" target="_blank"><img src="/webroot/web/images/general/icons/linkedin_round_40x40.png" alt="Bio-Rad LinkedIn"></a><a class="no-target-style" href="https://www.youtube.com/user/BioRadLifeScience" target="_blank"><img src="/webroot/web/images/general/icons/youtube-icon-rnd-40x40.png" alt="Bio-Rad YouTube"></a><a class="no-target-style" href="https://twitter.com/BioRadLifeSci" target="_blank"><img src="/webroot/web/images/general/icons/twitter_round_40x40.png" alt="Bio-Rad Twitter"></a><a class="no-target-style" href="https://www.facebook.com/biorad/" target="_blank"><img src="/webroot/web/images/general/icons/facebook-icon-rnd-40x40.png" alt="Bio-Rad Facebook"></a><a class="no-target-style" href="https://www.instagram.com/bioradlabs/" target="_blank"><img src="/webroot/web/images/general/icons/instagram-icon-rnd-40x40.png" alt="Bio-Rad Instagram"></a></div>');});</script>&copy; {0} Bio-Rad Laboratories, Inc. This page was last edited on {0} true True GTM-MJ2DN2S ru RU ru LSR life-science-research life-science-research /prd/ru/RU/direct/biorad?ts=1&cmd=OrdersWorkspaceDisplay /prd/ru/RU/direct/biorad?ts=1&cmd=QuotesWorkspaceDisplay /prd/ru/RU/direct/biorad?ts=1&cmd=InvoiceWorkspaceDisplay&_Tab=Invoice false false true false true true 1040988879 LHsYCOu_wQMQz_Ww8AM window.google_tag_params true 272-THL-329 username CartID_ WT_FPC <script type="text/javascript">$.ajax({url: document.location.protocol + '//munchkin.marketo.net/munchkin.js',dataType: 'script',cache: true,success: function() {Munchkin.init('044-CBN-599');}});</script> <script type="text/javascript" src="https://www.bio-rad.com/evportal/framework/skins/evolution/js/proxyMunchkin.js"></script> 2019 {"country":"ru","b2b_punch_out_mode":"","pageType":"App & Tech Detail","user_id":"","visitorLoginState":false,"visitorType":"","sessionType":"B2C","language":"ru","vertical":"LSR","b2b_org_id":""}