See How ddPCR Technology Builds on qPCR
Reliably Quantify Low-Abundance Targets With Easy Multiplexing
qPCR is a trusted method for amplifying and measuring nucleic acids. While it's still the right tool for many applications, ddPCR technology builds on that foundation for the cases where qPCR reaches its limits: detecting rare variants at low frequency, quantifying low-abundance targets without a standard curve, or getting reliable results from limited, precious samples. Use the comparison below to see exactly where each technology fits.
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| qPCR Technology | Droplet Digital™ PCR (ddPCR™) Technology | |
| Quantification | Provides reliable relative quantification using Cq values and a standard curve | Provides absolute quantification through direct molecule counts. No standard curve required |
| Precision | Delivers strong, consistent results at standard target concentrations | Extends precision down to very low target concentrations, without repetitive runs |
| Sensitivity | Effectively detects moderate-to-high abundance targets | Enables high sensitivity of low-abundance targets and precious samples |
| Multiplexing Capability | Performs best when amplification efficiency is well controlled (90–110%), which can limit multiplexing capabilities | Uses endpoint measurement to minimize efficiency bias, enabling easy, high-order multiplexing |
| Inhibitor Resistance | Reliable across many standard sample types | Greater tolerance to inhibitors for challenging sample matrices |
| Reproducibility | Supports repeatable results under well-controlled conditions with technical replicates | Demonstrated to deliver reproducible absolute quantification across runs and laboratories |
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Get Personalized Guidance From Our Technical Experts
Connect with a ddPCR specialist to find the right next step for your research.
- 1:1 technical seminar tailored to your applications
- Project consultation
- Live demo of the QX700 E, S, or HT ddPCR System
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One System, Built to Simplify Your ddPCR Workflow
Less complexity. More productivity.
Whether you're transitioning from qPCR or scaling high-throughput research, the QX700™ ddPCR Systems* offer integrated solutions that combine partitioning, amplification, and analysis in a streamlined workflow.
*For Research Use Only. Not for use in diagnostic procedures.
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Walkaway Operation
Start your run and move on while the system automates your ddPCR workflow from start to finish.
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Flexible by Design
Choose from three system configurations to match your throughput and research needs.
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More Data From Every Sample
Advanced 7-color multiplexing lets you analyze more targets while maximizing limited samples.
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Fast Results, Simplified Analysis
Go from sample to results with intuitive software and integrated quality control tools.
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Find the QX700 System That Fits Your Lab
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|---|---|---|---|
| QX700™ E System* A Modern Entry Point to ddPCR Technology | QX700™ S System* The New Standard for Scalable ddPCR Solutions | QX700™ HT System* A High-Throughput ddPCR Powerhouse | |
| Samples per run | Up to 48 | Up to 192 | Up to 384 |
| Plates per run | 1 plate/run | 1 to 4 plates/run | 1 to 8 plates/run |
| Thermal profiles/run | 3 | 12 | 24 |
| Max samples/ hours | 48 samples in ~2.5 hours | 192 samples in ~8 hours | 384 samples in ~8 hours |
| Time to result for 96 wells | ~5 hours | ~5 hours | ~2.5 hours |
| Continuous loading | — | Yes | Yes |
| Automation | Full | Full | Full |
*For Research Use Only. Not for use in diagnostic procedures.
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ddPCR Applications
Copy Number Variation (CNV)
Accurate CNV quantification in single wells.
Next Generation Sequencing
Amplification and accurate quantification of NGS libraries with validation of sequencing results.
Detection of Rare Sequences in Cancer and Disease Research
Detection of mutations and viral load below levels detectable by current tests.
Gene Expression Analysis
Detection of rare mRNAs and miRNAs with rapid turnover.
Cell & Gene Therapy Research
ddPCR assays for a range of applications including CAR transgene quantification.
Pathogen Detection and Microbiome Analysis Research
Viral load and pathogen detection in organ transplants, soil, and food.
Hematology-Oncology Research
Hematology-oncology solutions provide precise and accurate results without the need for standard curves.
Wastewater-Based Epidemiology
ddPCR assays for unlocking the full potential of wastewater testing and surveillance as a public health tool.